Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study

Kamath, Uday; Sheth, Hina; Ramesh, Sai G.; Singla, Keshav

doi:10.5005/jp-journals-10031-1049

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…Due to its mild solvent properties and antibacterial and antifungal qualities, TTO is considered an excellent treatment for open wounds, and sores. Recent research by Sheth et al demonstrated that TTO is as effective as sodium hypochlorite in cleansing root canal systems but less harmful (Sheth et al, 2013). TTO can also be used to treat various ailments, but it should be administered in therapeutic doses of 2.5 to 5% to maintain its antibacterial properties.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Antimicrobial Efficacy of German Chamomile Extract, Tea Tree Oil and Chlorhexidine as Root Canal Irrigants against E-faecalis and Streptococcus Mutans – An In Vitro Study

2023

IJAB

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Article HistoryBackground: In endodontic therapy, root canal irrigants are utilized to enhance mechanical debridement. However, conventional irrigants have certain drawbacks, such as being incapable of removing the smear layer, causing tissue irritation, toxicity, and unpleasant taste, especially in children. Therefore, there is a growing necessity to explore natural alternatives, such as herbal irrigants. This study sought to evaluate the antimicrobial effectiveness of German chamomile, tea tree oil (TTO), and chlorhexidine (CHX) as root canal irrigants against E. faecalis and Streptococcus mutans. Method: Fifteen test samples of German chamomile extract, tea tree oil, and chlorhexidine were taken. Indicator strains were grown in Brain Heart Infusion (BHI) agar at 37ºC for 24 hr. In preparation for the experiment, a 15 ml base layer of BHI agar was mixed with 300µl of each inoculum and placed in individual sterilized petri plates. Once the culture medium had solidified, four wells were created on each plate and subsequently filled with the designated testing materials. This methodology was employed to ensure methodological rigor and accuracy in the experimental process. In preparation for the experiment, a 15 ml base layer of BHI agar was mixed with 300µl of each inoculum and placed in individual sterilized petri plates. Once the culture medium had solidified, four wells were created on each plate and subsequently filled with the designated testing materials. This methodology was employed to ensure methodological rigor and accuracy in the experimental process. The plates were kept for 2 hours at room temperature for the diffusion of the material and then incubated at 37ºC for 48 hours. Antibacterial tests were repeated 5 times for homogeneity of the results. Results: The comparison of antimicrobial efficacy of chlorhexidine 2%, tea tree oil, and German chamomile showed a significant difference whereas the comparison between chlorhexidine 2% and tea tree oil was statistically insignificant. Conclusion: The effective reduction in the microbial count by all three test irrigants was observed to be significant. The antimicrobial efficacy of tea tree oil was found to be better than 2% chlorhexidine and German chamomile extract.

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Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of Antimicrobial Efficacy of German Chamomile Extract, Tea Tree Oil and Chlorhexidine as Root Canal Irrigants against E-faecalis and Streptococcus Mutans – An In Vitro Study

2023

IJAB

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“…36,52,54 Most of the studies are pertinent to the evaluation of antibacterial activity against E faecalis; as it is responsible for root canal failure and persistent infections. [64][65][66][67][68][69] The results of our search are summarized in tables 1 and 2 which clearly show that different herbal agents were studied for endodontic disinfection.…”

Section: Discussionmentioning

confidence: 99%

Role of Herbal Extracts in Root Canal Disinfection and Removal of Smear Layer: A Review

Munaga¹,

Halkai²,

Jarrah³

et al. 2022

JCMC

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Background: The success of endodontic treatment depends on the complete debridement and disinfection of canal space. Various chemicals have been used for canal disinfection. However, to overcome the adverse effects, toxicity issues, and microbial resistance of most conventional canal disinfectants, alternative agents are needed. Many herbal products have been studied and recommended for canal disinfection. This article aimed to review the role of herbal extracts in canal disinfection as an irrigant, intracanal medicament, and their efficacy in smear layer removal. Results: Studies were identified through search of the following electronic databases: PubMed, Science direct, Scopus and Google Scholar. The search was undertaken with keywords herbal agents/ products for root canal disinfection, Enterococcus faecalis, endodontics, smear layer, biofilm, herbal extracts and antimicrobial activity. The literature was reviewed for past 30 years (1990- till June 2020). The results showed that several in-vitro studies reported effective antimicrobial action of herbal extracts for canal disinfection, however, very less in-vivo or clinical studies have been reported to ascertain the use of these agents for endodontic therapy. Conclusions: The study concludes that herbal agents can be effectively used for canal disinfection. However, long-term, invitro, clinical, biocompatibility studies clinical trials are further needed to confidently use these products in endodontics. Herbal extracts can be used as substitute to existing root canal disinfectants due to their unique properties. Therefore, improving the endodontic success rate by preventing root canal failures, persistent and reinfections.

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“…Além disso a clorexidina é um antisséptico químico, o que segundo alguns autores, predispõe o surgimento de Research, Society andDevelopment, v. 10, n. 14, e59101420091, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i14.20091 resistências bacterianas. Em contrapartida, por ser uma substância natural, o óleo de Eucalyptus globulus não apresenta nenhuma restrição quanto ao seu uso (Sheth, Kamath, Ramesh, & Singla, 2013).…”

Section: Variáveisunclassified

Atividade do óleo essencial de Eucalyptus Globulus no controle de bactérias da cavidade oral

Monteiro¹,

Lima²,

Silva³

et al. 2021

RSD

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O óleo de Eucalyptus globulus, apresenta diversas propriedades farmacológicas. Dentre elas, destacam-se seus efeitos antissépticos, expectorantes, antifúngicos e antimicrobiano. Diante disto o presente trabalho teve o objetivo geral de avaliar a atividade do óleo de Eucalyptus globulus no controle de bactérias da cavidade oral e objetivos específicos de comparar o efeito o óleo de Eucalyptus globulus com efeito da clorexidina e determinar em quais cepas bacterianas o óleo de Eucalyptus globulus apresentava maior eficácia. A atividade antimicrobiana foi testada in vitro, em bactérias do grupo Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis) e Staphylococcus aureus (S. aureus) pelo método de difusão em ágar com a técnica de poços. Os poços foram preenchidos com as soluções de clorexidina 0,12% e óleo de Eucalyptus globulus em concentrações de 100%, 50%, 25%, 10%, 1% e 0,1% com a finalidade de comparar o efeito antimicrobiano pela inibição do crescimento. Os testes aconteceram em triplicata e a análise estatística foi realizada através do teste T de Student. Os dados foram organizados em tabelas para a demonstração dos resultados obtidos. Considerou-se como significante valores de p <0,05. Observou-se que o óleo de Eucalyptus globulus nas concentrações de 100%,50%,25% e 10% de maneira geral em todas as bactérias apresentaram efetividade semelhante ao da clorexidina. Pode-se concluir que o óleo de Eucalyptus globulus em suas variadas concentrações, apresentaram efeitos antimicrobianos, sua efetividade foi tão boa quanto o da clorexidina e em todas as cepas bacterianas estudadas este óleo apresentou ação antimicrobiana.

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Comparison of the Antibacterial Efficacy of Tea Tree Oil with 3% Sodium Hypochlorite and 2% Chlorhexidine against E. faecalis: An in vitro Study

Cited by 13 publications

References 10 publications

Comparative Evaluation of Antimicrobial Efficacy of German Chamomile Extract, Tea Tree Oil and Chlorhexidine as Root Canal Irrigants against E-faecalis and Streptococcus Mutans – An In Vitro Study

Comparative Evaluation of Antimicrobial Efficacy of German Chamomile Extract, Tea Tree Oil and Chlorhexidine as Root Canal Irrigants against E-faecalis and Streptococcus Mutans – An In Vitro Study

Role of Herbal Extracts in Root Canal Disinfection and Removal of Smear Layer: A Review

Atividade do óleo essencial de Eucalyptus Globulus no controle de bactérias da cavidade oral

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