Comparison of the Antimicrobial Properties of Silver Impregnated Vascular Grafts with and without Triclosan

Bérard, Xavier; Stecken, Laurent; Pinaquy, Jean-Baptiste; Cazanave, Charles; Pugès, Mathilde; Pereyre, Sabine; Bordenave, Laurence; M’Zali, Fatima

doi:10.1016/j.ejvs.2015.10.016

Cited by 43 publications

(38 citation statements)

References 25 publications

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“…The pure PHB‐VA film did not display any antibacterial activity. In contrast, prominent zones of inhibition were observed around all of the PHB‐VA–TCS blend film samples against E. coli and S. aureus; this confirmed the broad‐spectrum antimicrobial activity of the PHB‐VA–TCS blend films (Figure ) . The zone of inhibition increased with the increased incorporation of TCS in the PHB‐VA films, and PHB‐VA–T100 showed the largest zone of inhibition.…”

Section: Resultssupporting

confidence: 61%

Preparation and characterization of antimicrobial, biodegradable, triclosan‐incorporated polyhydroxybutyrate‐co‐valerate films for packaging applications

Sabharwal

Chattopadhyay

Singh

2018

J of Applied Polymer Sci

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The prospective application of polyhydroxybutyrate-co-valerate (PHB-VA) as a biodegradable polymer for packaging through the incorporation of triclosan (TCS) was studied via thermal, mechanical, and antimicrobial studies. The solvent-casting technique was used for the preparation of PHB-VA-TCS blend films. The blend films were characterized by differential scanning calorimetry, thermogravimetry, Fourier transform infrared spectroscopy, and mechanical properties measurements. The addition of TCS to PHB-VA resulted in increased film stretchability along with improved flexibility. The crystallization and melting temperatures of PHB-VA decreased with increasing concentration of TCS. Scanning electron microscopy images showed a homogeneous dispersion of TCS in PHB-VA. The bactericidal activities of the PHB-VA-TCS blend films were investigated against Escherichia coli and Staphylococcus aureus by the disc-diffusion method. The blend films exhibited higher antibacterial activities with increasing concentration of TCS. We concluded from the study that TCS could be successfully incorporated into PHB-VA to obtain flexible antimicrobial films for packaging applications.

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Section: Resultssupporting

confidence: 61%

Preparation and characterization of antimicrobial, biodegradable, triclosan‐incorporated polyhydroxybutyrate‐co‐valerate films for packaging applications

Sabharwal

Chattopadhyay

Singh

2018

J of Applied Polymer Sci

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“…Silver impregnated or rifampicin soaked PET grafts have been used for ISR following removal of the infected graft, but the level of evidence is low and most of the studies have been on the treatment of aortic infection. 232,233 In a study of 24 femoropopliteal bypasses replaced with silver impregnated PET grafts, the overall re-infection rate was 19%. 234 When rifampicin soaked grafts are used, the results of wound cultures (when available) should be considered, as data have shown that the micro-organism causing the infection was resistant or not susceptible to rifampicin in 31% of cases.…”

mentioning

confidence: 99%

Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

Chakfé

Diener

Lejay

et al. 2020

European Journal of Vascular and Endovascular Surgery

370

322

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DEDICATIONAfter studying medicine at Hanover Medical School and philosophy and social psychology at the Leibniz University Hanover, Omke E. Teebken joined the Christian Albrechts University in Kiel at the end of the 1990s as a research fellow at the Clinic for Cardiovascular Surgery headed by Professor Dr Axel Haverich, whom Omke E. Teebken later followed back to Hanover.In Hanover, besides working as a clinician, Omke E.Teebken was particularly active scientifically, contributing to the establishment of the then newly founded Leibniz Laboratories for Biotechnology and Artificial Organs (LEBAO). His work focused on regenerative medicine and tissue engineering, and subsequently he wrote his habilitation thesis in in this field. After basic training in cardiac surgery, he specialised clinically in vascular surgery and played a pioneering role in the development of this field. Before being appointed director of the Clinic for Vascular Surgery e Endovascular Surgery at the Peine Clinic in 2016, Omke E. Teebken headed the Vascular Surgery e Endovascular Surgery Division of the Department of Cardiothoracic, Thoracic, Transplantation and Vascular Surgery at Hanover Medical School. Professor Teebken was a highly appreciated, committed, and competent colleague and teacher.On 8 April 2019, Professor Teebken passed away after a short and severe illness. He was member and author of the ESVS guideline writing committee, an esteemed colleague, and friend.We will always honor his memory.

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“…2 Using an in vitro experimental protocol previously described, 3 it was shown that vascular grafts containing triclosan in addition to silver acetate (Intergard Synergy, Maquet, Orleans, France; IGSy) provided enhanced antimicrobial properties compared with grafts containing silver acetate only (Intergard Silver; IGS). 4 This advantage was demonstrated when grafts were contaminated with either Escherichia coli or methicillin resistant Staphylococcus aureus (MRSA); only the IGSy graft fulfilled the efficacy criterion for bactericidal activity at 24 h. For fungus Candida albicans, while both antimicrobial grafts achieved a 24 h bactericidal activity, only the IGSy graft demonstrated its bactericidal efficacy as early as 4 h following graft inoculation.…”

Section: Introductionmentioning

confidence: 99%

In vitro Evidence of Improved Antimicrobial Efficacy of Silver and Triclosan Containing Vascular Grafts Compared with Rifampicin Soaked Grafts

Bérard

Pugès

Pinaquy

et al. 2019

European Journal of Vascular and Endovascular Surgery

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WHAT THIS PAPER ADDS Thanks to efficient bactericidal activity against Gram positive and negative bacteria as well as yeasts, this study provides in vitro evidence in favour of a vascular graft containing silver and triclosan over a rifampicin soaked graft. The study confirms that rifampicin soaking exposes patients to the risk of hosting rifampicin resistant bacteria compromising the future efficacy of rifampicin. Objectives: The aim was to compare the antimicrobial efficacy of four different grafts: a standard graft (Intergard, IG), an IG graft soaked in rifampicin (IGrif), a silver impregnated graft (Intergard Silver, IGS), and a silver þ triclosan impregnated graft (Intergard Synergy, IGSy). Methods: This was a seven day in vitro study. The IG, IGrif, IGS, and IGSy grafts were each contaminated separately with the following microorganisms: Staphylococcus epidermidis, Methicillin resistant Staphylococcus aureus (MRSA), Escherichia coli, and Candida albicans from both clinical and American Type Culture Collection (ATCC) origins. The in vitro antimicrobial efficacy was evaluated by time to kill assays at T0, T24h, T48h, T72h, and T168h. Bactericidal activity was defined as >3 log 10 reduction factor (logRF). Additionally, Rifampicin, triclosan and silver resistance development were screened. Results: As anticipated for the non-antimicrobial IG, all microorganism strains proliferated. The IGSy and the IGS showed a seven day bactericidal efficacy (>3 logRF) for all tested microorganisms. This efficacy was confirmed at all time points for IGSy only, demonstrating faster bactericidal efficacy than IGS. The IGrif demonstrated a seven day bactericidal efficacy against the ATCC MRSA only, while showing no activity against C. albicans and ATCC E. coli. Regarding ATCC S. epidermidis, clinical MRSA and clinical E. coli, IGrif, although bactericidal at earlier time points, lost its antimicrobial efficacy at seven days leading to the emergence of rifampicin resistant mutants in four of six, two of six, and two of six assays, respectively. Mutant strains were also detected in ATCC MRSA in one of six assays. No triclosan or silver resistance has emerged at T7days. Conclusion: For all microorganisms tested, the Synergy graft combining silver with triclosan demonstrated a more sustainable and efficient seven day antimicrobial activity than the rifampicin soaked graft. The emergence of rifampicin resistant mutants suggests preference for a Synergy graft over a graft soaked in rifampicin, to prevent or treat an infection when a biological solution is not feasible.

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Comparison of the Antimicrobial Properties of Silver Impregnated Vascular Grafts with and without Triclosan

Abstract: In the in vitro conditions, the Synergy vascular graft combining silver with triclosan demonstrated better short-term antimicrobial activity than the silver graft for all micro-organisms tested.

Cited by 43 publications

References 25 publications

Preparation and characterization of antimicrobial, biodegradable, triclosan‐incorporated polyhydroxybutyrate‐co‐valerate films for packaging applications

Preparation and characterization of antimicrobial, biodegradable, triclosan‐incorporated polyhydroxybutyrate‐co‐valerate films for packaging applications

Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

In vitro Evidence of Improved Antimicrobial Efficacy of Silver and Triclosan Containing Vascular Grafts Compared with Rifampicin Soaked Grafts

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