Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Hoosein, Naseema M.; Brattain, Diane E.; McKnight, Mary K.; Childress, Karla E.; Chakrabarty, Subhas; Brattain, Michael G.

doi:10.1016/0304-3835(88)90014-6

Cited by 30 publications

(20 citation statements)

References 13 publications

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“…Primary rat intestinal epithelial cells were isolated from Sprague-Dawley rats by the method of Weiser (17). The recovery of a gradient of cells from crypt to villus was confirmed by measurement of sucrase activity (18), thymidine incorporation as described originally (17) 5) and the soluble extracts obtained as the supernatant after centrifugation ( 105,000 g for 60 min). Extracts were adjusted to a concentration of 1.0 mg/ml protein as determined by the method of Lowry et al (27) and either used directly for soft agar colony stimulation assays or first Iyophilized for competitive binding assays.…”

Section: Tems (I5)mentioning

confidence: 99%

“…The maintenance of mucosal integrity in the face of constant and rapid turnover of these cell populations suggests that exquisite mechanisms must exist to balance proliferative activity with both commitment to differentiation and loss of mature cells from the villus. A number of observations in both primary cells and established cell lines derived from the intestinal epithelium and human colon carcinomas indicate that soluble peptide growth factors may be essential in regulating proliferative activity in intestinal epithelial cells (1)(2)(3)(4)(5). A number of investigators have speculated that epidermal growth factor may play an important role in view of the detection of epidermal growth factor (EGF)' receptors on enterocytes (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

Koyama¹,

Podolsky

1989

J. Clin. Invest.

216

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Expression of transforming growth factor a (TGF a), and transforming growth factor 13 In addition to the transcripts identified in the primary intestinal cells, this cell line contained an additional larger TGF a transcript (4.8 kb) and smaller TGF 13 transcripts (2.2 and 1.8 kb). TGF a and TGF 13 may play a significant role in the regulation of the balance between proliferative and differentiated cell compartments in the intestinal epithelium through both autocrine and paracrine mechanisms. IntroductionThe intestinal mucosa is composed of a highly dynamic epithelial cell population in which proliferating cells are spatially segregated in the crypt from a gradient of increasingly differentiated cells present along the longitudinal axis of the villus. The maintenance of mucosal integrity in the face of constant and rapid turnover of these cell populations suggests that exquisite mechanisms must exist to balance proliferative activity

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Section: Tems (I5)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

Koyama¹,

Podolsky

1989

J. Clin. Invest.

216

View full text Add to dashboard Cite

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“…In contrast, the ectopic expression of the ETS1 gene in human colon cancer cell lines reduced the rate of the anchorage-independent growth in a dose dependent manner (Suzuki et al, 1995). It has been shown that RA reduces the anchorage-independent growth of the human colon cancer cells (Hoosein et al, 1988;Niles et al, 1988) suggesting that RA may reduce the tumorigenicity of human colon cancer cells through upregulation of ETS1 expression.…”

Section: Introductionmentioning

confidence: 99%

The Ets1 proto-oncogene is upregulated by retinoic acid: characterization of a functional retinoic acid response element in the Ets1 promoter

Raouf

Kola

et al. 2000

Oncogene

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“…Transformed cells, however, are either sensitive or refractory to the inhibitory effects of TGF,B1 (19,51). In BALB/ MK cells, TGFp1 selectively inhibits gene expression; expression of c-myc and KC is inhibited, c-fos expression is unaffected, and ,-actin expression is slightly augmented (8).…”

mentioning

confidence: 99%

Complex regulation of transforming growth factor beta 1, beta 2, and beta 3 mRNA expression in mouse fibroblasts and keratinocytes by transforming growth factors beta 1 and beta 2.

Bascom¹,

Wolfshohl²,

Coffey³

et al. 1989

Mol. Cell. Biol.

221

126

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Regulation of transforming growth factor Il1 (TGFII1), TGF(2, and TGF0i3 mRNAs in murine fibroblasts and keratinocytes by TGFIII and TGFI2 was studied. In quiescent AKR-2B fibroblasts, in which TGFOi induces delayed stimulation of DNA synthesis, TGFII1 autoregulation of TGFI1 expression was observed as early as 1 h, with maximal induction (25-fold) after 6 to 12 h. Increased expression of TGF(II mRNA was accompanied by increased TGFO protein production into conditioned medium of AKR-2B cells. Neither TGFI32 nor TGFI3 mRNA, however, was significantly induced, but both were apparently down regulated at later times by TGFI31. Protein synthesis was not required for autoinduction of TGFfII mRNA in AKR-2B cells. Nuclear run-on analyses and dactinomycin experiments indicated that autoregulation of TGF(l expression is complex, involving both increased transcription and message stabilization. In contrast to TGF(11, TGFj32 treatment of quiescent AKR-2B cells increased expression of TGFI1, TGFI82, and TGF,3 mRNAs, but with different kinetics. Autoinduction of TGF,I2 mRNA occurred rapidly with maximal induction at 1 to 3 h, enhanced TGFI83 mRNA levels were observed after 3 h, and increased expression of TGFI1 occurred later, with maximal mRNA levels obtained after 12 to 24 h. Nuclear run-on analyses indicated that TGFI2 regulation of TGF,I2 and TGF,3 mRNA levels is transcriptional, while TGFP2 induction of TGFI{1 expression most likely involves both transcriptional and posttranscriptional controls. In BALB/MK mouse keratinocytes, minimal autoinduction of TGF(H occurred at only the 12-and 24-h time points and protein synthesis was required for this autoinduction. The results of this study provide an example in which TGFI1 and TGF,2 elicit different responses and demonstrate that expressions of TGFII1, TGF,I2, and TGFj13 are regulated differently. The physiological relevance of TGFIV1 autoinduction in the context of wound healing is discussed.

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Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Cited by 30 publications

References 13 publications

Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

The Ets1 proto-oncogene is upregulated by retinoic acid: characterization of a functional retinoic acid response element in the Ets1 promoter

Complex regulation of transforming growth factor beta 1, beta 2, and beta 3 mRNA expression in mouse fibroblasts and keratinocytes by transforming growth factors beta 1 and beta 2.

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