Comparison of the BD GeneOhm Methicillin-Resistant
            <i>Staphylococcus aureus</i>
            (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from Intensive Care Unit Patients

Snyder, James W.; Munier, Gina K.; Johnson, Charles

doi:10.1128/jcm.01326-09

Cited by 53 publications

(40 citation statements)

References 34 publications

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“…Using this molecular screening approach, 95.2% of the samples could be reported negative based on the real-time PCR assays. Both assays had shown good sensitivities and negative predictive values in earlier studies, making this screening approach very useful for detection of MRSA-negative samples (15,21).…”

Section: Discussionmentioning

confidence: 99%

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A Rapid and High-Throughput Screening Approach for Methicillin-Resistant Staphylococcus aureus Based on the Combination of Two Different Real-Time PCR Assays

et al. 2014

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b Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that has been responsible for major nosocomial epidemics worldwide. For infection control programs, rapid and adequate detection of MRSA is of great importance. We developed a rapid and high-throughput molecular screening approach that consists of an overnight selective broth enrichment, followed by mecA, mecC, and S. aureus-specific (SA442 gene) real-time PCR assays, with subsequent confirmation using a staphylococcal cassette chromosome mec element (SCCmec)-orfX-based real-time PCR assay (GeneOhm MRSA assay) and culture. Here, the results of the screening approach over a 2-year period are presented. During this period, a total of 13,387 samples were analyzed for the presence of MRSA, 2.6% of which were reported as MRSA positive. No MRSA isolates carrying the mecC gene were detected during this study. Based on the results of the real-time PCR assays only, 95.2% of the samples could be reported as negative within 24 h. Furthermore, the performance of these real-time PCR assays was evaluated using a set of 104 assorted MRSA isolates, which demonstrated high sensitivity for both the combination of mecA and mecC with SA442 and the BD GeneOhm MRSA assay (98.1% and 97.1%, respectively). This molecular screening approach proved to be an accurate method for obtaining reliable negative results within 24 h after arrival at the laboratory and contributes to improvement of infection control programs, especially in areas with a low MRSA prevalence.

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Section: Discussionmentioning

confidence: 99%

“…Unfortunately, the SCCmec-orfXbased assays have proved not to be able to detect all MRSA isolates correctly. In the past, mecA dropouts have been reported, resulting in false-positive MRSA results (12,15). Moreover, because of the high diversity in SCCmec-orfX sequences, correct detection remains a challenge (16,17).…”

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confidence: 99%

A Rapid and High-Throughput Screening Approach for Methicillin-Resistant Staphylococcus aureus Based on the Combination of Two Different Real-Time PCR Assays

et al. 2014

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“…All suspected MRSA isolates detected by chromogenic medium were confirmed by a real-time PCR technique using the BD GeneOhmTM MRSA assay (BD Diagnostics, Franklin Lakes, USA) following the manufacturer's instructions. The BD GeneOhm MRSA PCR assay has a sensitivity and specificity of 100% and 97%, respectively, compared to culture by use of BBL CHROMagar MRSA [7]. Quality control for antimicrobial susceptibility testing was performed with S. aureus ATCC 25923.…”

Section: Methodsmentioning

confidence: 99%

Vancomycin susceptibility trends of methicillin-resistant Staphylococcus aureus isolated from burn wounds: a time for action

Zorgani

Elahmer

Abaid³

et al. 2015

J Infect Dev Ctries

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) with reduced susceptibility to vancomycin poses a threat for patients in burn units throughout the world. This study aimed to investigate the reduced susceptibility to vancomycin of MRSA isolated from wounds of patients admitted to the Burns and Plastic Surgery Centre in Tripoli, Libya. Methodology: All isolates were initially identified by chromagen medium then confirmed by PCR. The minimum inhibition concentration (MIC) was determined by E-test glycopeptide resistance detection (GRD). Results: During the study, 210 isolates were obtained from 560 patients representing 132 (62.9%) and 78 (37.1%) of total samples received during years 2009 and 2010, respectively. MIC levels for vancomycin ranged from 0.5 to 2 µg/ml during the study, 13% of isolates displayed MIC of 1.5 µg/ml and 9% of the isolates displayed 2 µg/ml. Although MRSA isolates decreased dramatically during 2010 (37.1%) compared to 2009 (62.9%), overall, there was a significant increase in the proportion of MRSA isolates exhibiting higher vancomycin MICs during 2010 compared to 2009 (P = 0.0155). There was a significant increase of MICs at 1 µg/ml during 2010 compared with 2009 (P = 0.36). No vancomycin intermediate or resistant strains were found. Conclusion: There was a significant increase in the proportion of MRSA isolates exhibiting higher vancomycin MICs. We recommend that MRSA isolates should be monitored. Furthermore, implementation of infection control measures is urgently needed to prevent the spread of MRSA.

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“…Previous studies have shown mostly high sensitivity of PCR up to 97.3% compared to the disk diffusion method [12], Some other studies shows 70.3% [23], While other study gave 59.2% for oxacillin resistant MRSA and 55.2% for cefoxitin resistant MRSA [22,24]. In this study the sensitivity was 55% indicating that the methicillin resistant in S. aureus may not come from mecA gene, it may come from over production of β-lactamase by other gene which have mutation in its regulatory gene, including various length sequences deletion, or conjugation and transformation occur between bacterial cells causing constitutive expression for the β-lactamase [15].…”

Section: Resultsmentioning

confidence: 99%

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

Kareem

2013

JNUS

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Staphylococcusaureusis avirulence pathogenic bacterium.Detection of methicillin-resistant S. aureus(MRSA) using conventional culture and biochemical methods is labor and time consuming. Many MRSA isolates are heterogeneously resistant to ß-lactams, for example only 1 daughter cell out of 10 4 to 10 6 cells appears phenotypically resistant when routine antimicrobial susceptibility tests are performed. In this work two genes were used for detection of MRSA in urine, boil and nasal swabs using conventional PCR, mecA gene (533bp) were used for detection of methicillin resistant and femA gene (318bp) were used for S.aureus identification. It was found that not all resistant S. aureus tested with disk diffusion method carry the mecA gene that caused the resistant phenomena there were only 55% of all MRSA carrying mecA gene while femA gene gave 100% positive for S. aureus and this will lead us to appoint that penicillin-bindingprotein 2a (PBP2a) produced by mecA gene is not the only cause of resistant phenomena.

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Comparison of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from Intensive Care Unit Patients

Cited by 53 publications

References 34 publications

A Rapid and High-Throughput Screening Approach for Methicillin-Resistant Staphylococcus aureus Based on the Combination of Two Different Real-Time PCR Assays

A Rapid and High-Throughput Screening Approach for Methicillin-Resistant Staphylococcus aureus Based on the Combination of Two Different Real-Time PCR Assays

Vancomycin susceptibility trends of methicillin-resistant Staphylococcus aureus isolated from burn wounds: a time for action

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

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