Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods

Abstract

in the prevention of oxidative damage in human cells [Wang et al., 2012a]. In general, polysaccharides are bound to other components like protein, lipids, lignin, and some inorganic minerals in cells. Thus, polysaccharide extraction and isolation from various sources is the most important task in the investigation and application of bioactive polysaccharides. The extraction technique used may signifi cantly infl uence the yield, physical characteristics, and biological activities of the polysaccharides. Hot-water extraction (HWE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction, enzyme-assisted extraction (EAE), maceration, and heat refl ux with solvents are the main methods for polysaccharide extraction from natural resources [Fan et al., 2015]. HWE is traditionally used because it is a convenient, fast, environment-friendly, and economical technology [Liu et al., 2016]. UAE has been widely used to extract bioactive components from various plant materials based on mass transfer intensifi cation, cell collapse, increased penetration, and capillary effects [Yin et al., 2016]. EAE can be used to release natural functional compounds from plant materials, owing to cell wall degradation that facilitates polysaccharide dissolution into the solvent [Pan et al., 2015]. In this study, crude polysaccharides were extracted from C. sinensis by various extraction methods and their health-related properties were measured in order to improve their availability to functional food materials. The crude polysaccharide fractions from C. sinensis were extracted using hot water, ultrasonic-assisted, and enzyme-assisted methods. Biological activities such as antioxidant activity, α-amylase in

Purification and identification of an antioxidant peptide from perilla seed (Perilla frutescens) meal protein hydrolysate

“…ABTS radical scavenging activity was measured according to Oh and Yoon () with modifications. The ABTS radical scavenging activity reaction was an aqueous solution of 7 mM ABTS with 2.45 mM potassium persulfate, stored in the dark at room temperature for 12 hr.…”

Biological activity of enzymatic hydrolysates and the membrane ultrafiltration fractions from perilla seed meal protein

“…Measurement of antioxidant activity. The ability of PPH and its fractions to scavenge 2,2'-azino-bis(3ethylbenzothiazoline-6-sulfonic acid (ABTS + ) radicals was measured according to Oh and Yoon (2018). Each sample (240 µl) was added to 80 µl of ABTS + solution, which was prepared by mixing 0.7 mM of ABTS stock solution with 2.45 mM potassium persulfate.…”

Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

“…ese polyphenols have been reported to alleviate oxidative stress, to reduce chronic low-grade inflammation, and to inhibit lipogenesis, thereby improving insulin resistance and reducing risk of cardiovascular diseases [3,13,49,52,53]. Methanol extract of aerial parts of OB contains high levels of gallic acid, ellagic acid, caffeic acids, quercetin, and kaempferol [54]. CS sprouts contain Evidence-Based Complementary and Alternative Medicine abundant limonoids, triterpenoids, and flavonoids, such as quercetin and quercitrin [33,41,55].…”