Comparison of the characteristics and multipotential and in vivo cartilage formation capabilities between porcine adipose-derived stem cells and porcine skin-derived stem cell–like cells

Hwang, In-Sul; Bae, Hyo-Kyung; Cheong, Hyeonsook

doi:10.2460/ajvr.76.9.814

Cited by 4 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,28,29 These include comparable morphology, proliferative capacity, alkaline phosphatase activity, cell surface marker expression, metabolic pathways, biologic functions, and transcription factors. 10,23,30 pASCs have often been compared side-by-side with porcine stem cells from these other tissue sources, revealing characteristics and comparable multilineage differentiation abilities as well. 9,23,28 While knowledge of the differentiation abilities of pASCs is currently limited to a few reports, porcine bone marrow-derived stem cells (pBMSCs) have been differentiated into myocytes, 31 endothelial cells, 32 and epithelial cells.…”

Section: Multilineage Differentiation Abilitiesmentioning

confidence: 99%

Properties of porcine adipose-derived stem cells and their applications in preclinical models

Arrizabalaga

Nollert

2017

Adipocyte

View full text Add to dashboard Cite

Adipose-derived stem cells represent a reliable adult stem cell source thanks to their abundance, straightforward isolation, and broad differentiation abilities. Consequently, human adipose-derived stem cells (hASCs) have been used in vitro for several innovative cellular therapy and regenerative medicine applications. However, the translation of a novel technology from the laboratory to the clinic requires first to evaluate its safety, feasibility, and potential efficacy through preclinical studies in animals. The anatomy and physiology of pigs and humans are very similar, establishing pigs as an attractive and popular large animal model for preclinical studies. Knowledge of the properties of porcine adipose-derived stem cells (pASCs) used in preclinical studies is critical for their success. While hASCs have been extensively studied this past decade, only a handful of reports relate to pASCs. The aim of this concise review is to summarize the current findings about the isolation of pASCs, their culture, proliferation, and immunophenotype. The differentiation abilities of pASCs and their applications in porcine preclinical models will also be reported.

show abstract

Section: Multilineage Differentiation Abilitiesmentioning

confidence: 99%

Properties of porcine adipose-derived stem cells and their applications in preclinical models

Arrizabalaga

Nollert

2017

Adipocyte

View full text Add to dashboard Cite

show abstract

“…Cells from each pig were cultured separately and passaged once or twice with culture medium changed every two days. MSC characteristics of pSSCs were confirmed by fluorescence-activated cell sorting analysis as described previously [10].…”

Section: Methodsmentioning

confidence: 99%

“…For analysis of adipocytes generated during osteogenic differentiation, ORO staining and quantification were conducted as described previously [10]. Briefly, pSSCs were stained with a 0.6% ORO (Sigma-Aldrich) solution (w/v) for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we reported on differentiation induction of porcine skin-derived stem cells (pSSCs) into three mesodermal cell lineages in vitro [10]. In previous studies, lipid (such as free fatty acids) droplets have been generated not only during adipogenic differentiation but also during chondrogenic differentiation [3] and osteogenic differentiation of pSSCs (data not shown).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Correlation of spontaneous adipocyte generation with osteogenic differentiation of porcine skin-derived stem cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

The objective of this study was to examine effects of spontaneous adipocyte generation on osteogenic differentiation of porcine skin-derived stem cells (pSSCs). Correlation between osteogenic differentiation and adipocyte differentiation induced by osteocyte induction culture was determined using different cell lines. Osteogenic differentiation efficiency of pSSCs was then analyzed by controlling the expression of adipocyte-specific transcription factors during osteogenic induction culture. Among four cell lines, pSSCs-II had the lowest lipid droplet level but the highest calcium content (p < 0.05). It also expressed significantly low levels of peroxisome proliferator-activated receptor gamma 2 (PPARγ2) and adipocyte protein 2 (aP2) mRNAs but very high levels of runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) mRNAs as osteogenic makers (p < 0.05). Oil red O extraction was increased by 0.1 µM troglitazone (TGZ) treatment but decreased by 50 µM bisphenol A diglycidyl ether (BADGE) (p < 0.05). Calcium content was drastically increased after BADGE treatment compared to that in osteogenic induction control and TGZ-treated pSSCs (p < 0.05). Relative expression levels of PPARγ2 and aP2 mRNAs were increased by TGZ but decreased by BADGE. Expression levels of Rucx2 and ALP mRNAs, osteoblast-specific marker genes, were significantly increased by BADGE treatment (p < 0.05). The expression level of BCL2 like 1 was significantly higher in BADGE-treated pSSCs than that in TGZ-treated ones (p < 0.05). The results demonstrate that spontaneous adipocyte generation does not adversely affect osteogenic differentiation. However, reducing spontaneous adipocyte generation by inhibiting PPARγ2 mRNA expression can enhance in vitro osteogenic differentiation of pSSCs.

show abstract

“…In mammals, WAT depots occupy different anatomical locations and demonstrate morphological and functional heterogeneity [26]. In the pig, abdominal and dorsal regions of subcutaneous fat have been the most frequently used source of ASCs [27][28][29][30]. There is only one report that refers to the caudal part of the body for ASC isolation [31].…”

Section: Basic Biology Of Ascs Isolated From Livestockmentioning

confidence: 99%

Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science

Bukowska

Szóstek‐Mioduchowska

Kopcewicz

et al. 2020

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Adipose-derived stem cells (ASCs) isolated from domestic animals fulfill the qualitative criteria of mesenchymal stem cells, including the capacity to differentiate along multiple lineage pathways and to self-renew, as well as immunomodulatory capacities. Recent findings on human diseases derived from studying large animal models, have provided evidence that administration of autologous or allogenic ASCs can improve the process of healing. In a narrow group of large animals used in bioresearch studies, pigs and horses have been shown to be the best suited models for study of the wound healing process, cardiovascular and musculoskeletal disorders. To this end, current literature demonstrates that ASC-based therapies bring considerable benefits to animal health in both spontaneously occurring and experimentally induced clinical cases. The purpose of this review is to provide an overview of the diversity, isolation, and characterization of ASCs from livestock. Particular attention has been paid to the functional characteristics of the cells that facilitate their therapeutic application in large animal models of human disease. In this regard, we describe outcomes of ASCs utilization in translational research with pig and horse models of disease. Furthermore, we evaluate the current status of ASC-based therapy in veterinary practice, particularly in the rapidly developing field of equine regenerative medicine. In conclusion, this review presents arguments that support the relevance of animal ASCs in the field of regenerative medicine and it provides insights into the future perspectives of ASC utilization in animal husbandry. Graphical abstract

show abstract

Comparison of the characteristics and multipotential and in vivo cartilage formation capabilities between porcine adipose-derived stem cells and porcine skin-derived stem cell–like cells

Abstract: Results suggested that pSSCs may be a viable alternative to pASCs as a source of progenitor cells for tissue engineering in regenerative medicine.

Cited by 4 publications

References 29 publications

Properties of porcine adipose-derived stem cells and their applications in preclinical models

Properties of porcine adipose-derived stem cells and their applications in preclinical models

Correlation of spontaneous adipocyte generation with osteogenic differentiation of porcine skin-derived stem cells

Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science

Contact Info

Product

Resources

About