Comparison of the chondroprotective effect of a novel hydrogel compound and traditional hyaluronate on rat cartilage in a papain-induced osteoarthritis model

Sukur, Erhan; Talu, Canan Kelten; Akman, Yunus Emre; Çirçi, Esra; Öztürkmen, Yusuf; Tüzüner, Tolga

doi:10.1016/j.aott.2016.07.008

Cited by 13 publications

(11 citation statements)

References 26 publications

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“…Currently it has been shown that intra-articular HA, NAG and CS application was safe and efficacious in OA trials, experimental animal models and in vitro studies. 19 , 36 , 37 , 38 In our experimental rat model, we also found out that intra-articular application of HA, NAG and CS is effective according to our histological evaluation.…”

Section: Discussionsupporting

confidence: 54%

“… 18 In a previous study, it is found out that a recently introduced intra-articular injectable hydrogel form of a compound of HA (hyaluronate) (36 mg/2.25 mL), CS (chondroitin sulfate) (45 mg/2.25 mL) and NAG (N-acetyl- d -glucosamine) (9 mg/2.25 mL) is more chondroprotective to rats' cartilage when compared to HA during the early stages of osteoarthritis with respect to preserving and healing articular surfaces, cellular abnormality and proteoglycan content. 19 Despite of the presence of many studies on the intra-articular use of HA, oral NAG and CS combination, there is no experimental data to evaluate the intra-articular application following micro-fracture application and the effects on hyaline cartilage of these drugs. The purpose of this experimental study was to compare the effects of intra-articularly injected HA and intra-articular injectable hydrogel form of a compound of HA, NAG and CS which are applied following micro-fracture procedure in an full-thickness chondral defect model in rat, through the histopathological parameters.…”

Section: Introductionmentioning

confidence: 99%

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The comparison of the effects of a novel hydrogel compound and traditional hyaluronate following micro-fracture procedure in a rat full-thickness chondral defect model

Akman¹,

Sukur²,

Senel³

et al. 2017

Acta Orthopaedica et Traumatologica Turcica

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PurposeThe aim of this experimental study was to investigate the impact of HA–CS–NAG compound (hyaluronate, sodium chondroitin sulfate, N-acetyl-d-glucosamine) on the quality of repair tissue after micro-fracture and to compare it with HA (hyaluronat), in a rat full-thickness chondral defect model.MethodsFull-thickness chondral defects were created in a non-weight bearing area by using a handle 2.7-mm drill bit, in the right knees of 33 Sprague–Dawley rats. Each specimen then underwent micro-fracture using a needle. Two weeks after surgery, 3 groups were randomly formed among the rats (n = 33). In Group 1, 0.2 mL of sterile saline solution (0.9%) was injected. In Group 2, 0.2 mL HA with a mean molecular weight of 1.2 Mda was injected. In Group 3, 0.2 mL of HA–CS–NAG compound (hyaluronate, sodium chondroitin sulfate, N-acetyl-d-glucosamine) was injected. The injections were applied on the 14th, the 21st and the 28th postoperative days. All rats were sacrificed on the 42nd postoperative day. Histological analysis of the repair tissue was performed for each specimen by two blinded observers using Wakitani scoring system.ResultsThere was significantly improved repair tissue in both Group 3 and Group 2 when compared with Group 1. Group 3 showed statistically significant improvement in terms of ‘cell morphology’ and ‘integration of donor with host’ when compared to Group 2 (p < 0.001).ConclusionIntra-articular injection of HA–CS–NAG compound after micro-fracture results in significantly improved repair tissue in rats' chondral defects when compared to HA regarding the donor integration and cell morphology.

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Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

The comparison of the effects of a novel hydrogel compound and traditional hyaluronate following micro-fracture procedure in a rat full-thickness chondral defect model

Akman¹,

Sukur²,

Senel³

et al. 2017

Acta Orthopaedica et Traumatologica Turcica

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“…HA protects collagen fibrils, cells of the articular surface, synovial tissue, capsule, and ligament from mechanical damage. Moreover, intra-articular HA knee injections have been shown to have a chondroprotective effect in an early knee OA rat model 11. HA has been widely used in the management of knee OA, and the efficacy of this treatment has been confirmed in several meta-analyses 9,12–14…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive effects of hyaluronic acid on monoiodoacetate-induced ankle osteoarthritis in rats

Jimbo¹,

Terashima²,

Teramoto³

et al. 2019

JPR

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PurposeAnkle osteoarthritis (OA) causes significant pain and debilitation; yet, its underlying mechanisms remain unclear. Clinically, hyaluronic acid (HA) is widely used to treat OA. The present study aimed to investigate the roles of HA in pain-related behavior, joint function, swelling, and pathological changes in cartilage in a rat model of monoiodoacetate (MIA)-induced ankle OA.Materials and methodsMale Sprague Dawley rats were assigned to three experimental groups as follows: 1) MIA rats injected with 1 mg MIA in the right tibiotarsal joint for two consecutive days; 2) sham rats injected with saline instead of MIA; and 3) MIA-HA rats injected with HA in the tibiotarsal joint at 7, 14, and 21 days after MIA injection. Joint swelling, range of motion (ROM), and pain-related behavior were evaluated 1 day before and on the 7th, 14th, 21st, and 28th day after MIA or saline injection. Pathological changes in the ankle joint were assessed 28 days after MIA or saline injection.ResultsNo significant difference in the degree of ankle swelling or ROM reduction was observed between MIA rats and MIA-HA rats. However, compared with those in MIA rats, mechanical and thermal hypersensitivity was significantly reduced and stride length significantly improved in MIA-HA rats. Histologic analysis revealed that cartilage degeneration was significantly suppressed in MIA-HA rats compared with that in MIA rats, reflecting the chondroprotective effects of HA.ConclusionHA improved pain-related behavior and stride length and suppressed MIA-induced cartilage degeneration. HA may thus inhibit OA progression and suppress peripheral and/or central sensitization.

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“…It has been stated that HA gels with proteoglycan could alleviate the onset of OA after knee injury, and in situ cross-linkable HA hydrogel could improve cartilage regeneration [10, 11]. Solchaga et al showed the fibronectin-coated hyaluronan-based scaffold could organize the natural response and facilitate the integration of the neo-cartilage with the adjacent tissue [12].…”

Section: Discussionmentioning

confidence: 99%

Icariin-conditioned serum engineered with hyaluronic acid promote repair of articular cartilage defects in rabbit knees

Zhang

et al. 2019

BMC Complement Altern Med

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Background Osteochondral defects mostly occur as a result of trauma or articular degeneration. The poor regenerative ability of articular cartilage remains osteochondral defects are a tricky problem to deal with. The modern treatment strategies mainly focus on cartilage tissue engineering with bioactive materials. In this study, we aimed to develop icariin conditioned serum (ICS) together with hyaluronic acid (HA) and determine their ability in reparing osteochondral tissue in a critical-sized defect in rabbit knees. Methods Primary chondrocytes were incubated with serum conditioned with icariin at different concentrations, then cell proliferation rates and glycosaminoglycan (GAG) secretion were detected. Rabbits were treated with intra-articular injection of 0.5 mL normal saline (NS), ICS, HA and ICS + HA in the right knee joint, respectively. ICRS scores were used to assess the macroscopic cartilage regeneration. Histological and immunohistochemical analysis including H&E, Safranin O, toluidine blue and collagen II staining were used to determine the repair of cartilage and the regeneration of chondrocytes. Results Icariin at a low dose of 0.94 g/kg was identified to have significantly promoted the proliferation of chondrocytes and enhance the secretion of GAG. Femoral condyle from rabbits treated by ICS together with HA was observed to be integrated with native cartilage and more subchondral bone regeneration. ICS together with HA could promote repair of the cartilage defect and increase the neoformation of cartilage. Conclusions These results demonstrated the potential of ICS combined with HA to promote reparative response in cartilage defects and the possible application in bioactive material based cartilage regeneration therapies. Electronic supplementary material The online version of this article (10.1186/s12906-019-2570-0) contains supplementary material, which is available to authorized users.

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Comparison of the chondroprotective effect of a novel hydrogel compound and traditional hyaluronate on rat cartilage in a papain-induced osteoarthritis model

Cited by 13 publications

References 26 publications

The comparison of the effects of a novel hydrogel compound and traditional hyaluronate following micro-fracture procedure in a rat full-thickness chondral defect model

The comparison of the effects of a novel hydrogel compound and traditional hyaluronate following micro-fracture procedure in a rat full-thickness chondral defect model

Antinociceptive effects of hyaluronic acid on monoiodoacetate-induced ankle osteoarthritis in rats

Icariin-conditioned serum engineered with hyaluronic acid promote repair of articular cartilage defects in rabbit knees

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