Abstract. Let L be the infinitesimal generator of an analytic semigroup on L 2 (R n ) with suitable upper bounds on its heat kernels. In Auscher, Duong, and McIntosh (2005) and , a Hardy space H 1 L (R n ) and a BMO L (R n ) space associated with the operator L were introduced and studied. In this paper we define a class of H p L (R n ) spaces associated with the operator L for a range of p < 1 acting on certain spaces of Morrey-Campanato functions defined in New Morrey-Campanato spaces associated with operators and applications by Duong and Yan (2005), and they generalize the classical H p (R n ) spaces. We then establish a duality theorem between the H p L (R n ) spaces and the Morrey-Campanato spaces in that same paper. As applications, we obtain the boundedness of fractional integrals on H p L (R n ) and give the inclusion between the classical H p (R n ) spaces and the H p L (R n ) spaces associated with operators.