Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel

Guibaud, Gilles; Comte, Sophie; Bordas, François; Dupuy, Sèverine; Baudu, Michel

doi:10.1016/j.chemosphere.2004.10.028

Cited by 269 publications

(154 citation statements)

References 36 publications

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“…Using adsorption isotherms and Freudlich model it has been demonstrated [85] that the affi nity of sludge EPS for metals was in the order Zn>Cu>Cr>Cd>Co>Ni>CrO 4 . The metal (Cd, Pb and Ni) complexation potential of EPS extracted from activated sludges and those produced by the pure cultures of bacteria isolated from the same sludges was compared [46]. Biosorption order for the metals was Pb> Ni >Cd irrespective of the origin of the EPS.…”

Section: Escherichia Coli Phl628mentioning

confidence: 99%

“…and low quantities of polysaccharides and uronic acids in comparison with EPS extracted from activated sludges [46]. Differences in protein composition of capsular, biofi lm and free EPS of Pseudomonas NCIMB 2021 were confi rmed by polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) spectroscopic analysis [44].…”

Section: Compositional Analysismentioning

confidence: 99%

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Microbial extracellular polymeric substances: central elements in heavy metal bioremediation

2008

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Extracellular polymeric substances (EPS) of microbial origin are a complex mixture of biopolymers comprising polysaccharides, proteins, nucleic acids, uronic acids, humic substances, lipids, etc. Bacterial secretions, shedding of cell surface materials, cell lysates and adsorption of organic constituents from the environment result in EPS formation in a wide variety of free-living bacteria as well as microbial aggregates like biofi lms, biofl ocs and biogranules. Irrespective of origin, EPS may be loosely attached to the cell surface or bacteria may be embedded in EPS. Compositional variation exists amongst EPS extracted from pure bacterial cultures and heterogeneous microbial communities which are regulated by the organic and inorganic constituents of the microenvironment. Functionally, EPS aid in cell-to-cell aggregation, adhesion to substratum, formation of fl ocs, protection from dessication and resistance to harmful exogenous materials. In addition, exopolymers serve as biosorbing agents by accumulating nutrients from the surrounding environment and also play a crucial role in biosorption of heavy metals. Being polyanionic in nature, EPS forms complexes with metal cations resulting in metal immobilization within the exopolymeric matrix. These complexes generally result from electrostatic interactions between the metal ligands and negatively charged components of biopolymers. Moreover, enzymatic activities in EPS also assist detoxifi cation of heavy metals by transformation and subsequent precipitation in the polymeric mass. Although the core mechanism for metal binding and / or transformation using microbial exopolymer remains identical, the existence and complexity of EPS from pure bacterial cultures, biofi lms, biogranules and activated sludge systems differ signifi cantly, which in turn affects the EPS -metal interactions. This paper presents the features of EPS from various sources with a view to establish their role as central elements in bioremediation of heavy metals.

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Section: Escherichia Coli Phl628mentioning

confidence: 99%

Section: Compositional Analysismentioning

confidence: 99%

Microbial extracellular polymeric substances: central elements in heavy metal bioremediation

2008

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“…16−18 The main structural components of bacterial biofilms are extracellular polymeric substances (EPS), composed of heterogeneous materials that include proteins, lipids, and polysaccharides. 19,20 EPS contain numerous functional groups, such as carboxyl, phosphoryl, amine, and hydroxyl, and thus potentially can act as a binding or nucleation site for heavy metals. 21,22 In addition, it was proposed that outer-membrane cytochromes (e.g., MtrC and OmcA in Shewanella oneidensis MR-1), that mediate enzymatic U reduction, are also localized in the EPS.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Uranium Reduction and Immobilization in Desulfovibrio vulgaris Biofilms

Stylo

Neubert

Roebbert

et al. 2015

Environ. Sci. Technol.

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The prevalent formation of noncrystalline U(IV) species in the subsurface and their enhanced susceptibility to reoxidation and remobilization, as compared to crystalline uraninite, raise concerns about the long-term sustainability of the bioremediation of U-contaminated sites. The main goal of this study was to resolve the remaining uncertainty concerning the formation mechanism of noncrystalline U(IV) in the environment. Controlled laboratory biofilm systems (biotic, abiotic, and mixed biotic−abiotic) were probed using a combination of U isotope fractionation and X-ray absorption spectroscopy (XAS). Regardless of the mechanism of U reduction, the presence of a biofilm resulted in the formation of noncrystalline U(IV). Our results also show that biotic U reduction is the most effective way to immobilize and reduce U. However, the mixed biotic−abiotic system resembled more closely an abiotic system: (i) the U(IV) solid phase lacked a typically biotic isotope signature and (ii) elemental sulfur was detected, which indicates the oxidation of sulfide coupled to U(VI) reduction. The predominance of abiotic U reduction in our systems is due to the lack of available aqueous U(VI) species for direct enzymatic reduction. In contrast, in cases where bicarbonate is present at a higher concentration, aqueous U(VI) species dominate, allowing biotic U reduction to outcompete the abiotic processes.

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“…the absorption band at 1101 cm -1 in the spectrum of SEPS might show the existence of uronic in SEPS. The presence of CH 2 in the FTIR spectra of SEPS and carboxylic groups in BEPS indicated the presence of lipid (12). The presence of the "fingerprint zone" (<1000 cm -1 ) may indicate that nucleic acids also exist in EPS.…”

Section: Ftir Spectroscopic Analysismentioning

confidence: 99%

Lead Complexation of Soluble and Bound Extracellular Polymeric Substances from Activated Sludge: Characterized with Fluorescence Spectroscopy and Ftir Spectroscopy

Song

Pan

Zhang

2012

Biotechnology & Biotechnological Equipment

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Comparison of the complexation potential of extracellular polymeric substances (EPS), extracted from activated sludges and produced by pure bacteria strains, for cadmium, lead and nickel

Cited by 269 publications

References 36 publications

Microbial extracellular polymeric substances: central elements in heavy metal bioremediation

Microbial extracellular polymeric substances: central elements in heavy metal bioremediation

Mechanism of Uranium Reduction and Immobilization in Desulfovibrio vulgaris Biofilms

Lead Complexation of Soluble and Bound Extracellular Polymeric Substances from Activated Sludge: Characterized with Fluorescence Spectroscopy and Ftir Spectroscopy

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