chemical nature of phospholipids in rat lung tissue and alveolar wash was studied using column chromatographies on silicic acid and DEAE-cellulose and thin-layer chromato graphy followed by gas chromatography to determine the fatty acid composition of the major phospholipid fractions. It was found that alveolar phospholipids obtained by lung lavage differed from those in lung tissue in several aspects. Predominant phospholipid from both sources was lecithin which composed 68% of alveolar phospholipids and 52% of lung tissue phospholipids. Alveolar lecithin contained much higher disaturated species than lung tissue. Lipids X1, X2 and X3 were found in higher proportions in alveolar wash. The X3 seemed to be identical with cardiolipin from the data of Rf values of intact lipids as well as deacylated products, and elution pattern with DEAE-cellulose. The acidic lipid X2 seemed to be probably phosphatidylglycerol. Lipid X1 could not be identified. The present results suggested that lecithin and the three acidic phospholipids could be secreted more preferentially into alveolar cavity than other lipids, such as neutral lipids, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and sphingomyelin. alveolar phospholipids, dipalmi toyl lecithin It has been reported that the alveolar lining layer of the lung, washed out by saline, contains a surface active material (surfactant) which lowers alveolar surface tension and decreases the tendency of the lung to collapse. A number of studies have been presented on the chemical nature and metabolism of this lung surfactant. It has generally been accepted that so-called dipalmitoyllecithin, one of the molecular species of lecithin, in the lung and alveolar wash is the principal compo nent of the surfactant (Pattle 1958, Brown et al. 1959, Klaus et al. 1961, Avery 1959. Morgan et al. (1965) reported that alveolar lipids in dog pulmonary wash contained significant amounts of phosphatidyldimethylethanolamine which might contribute to the formation of dipalmitoyllecithin with methyltransferase. How ever, several investigators have reported recently the presence of phosphatidyl glycerol and lyso-bis-phosphatidic acid, but they stated that they could not detect phosphatidyldimethylethanolamine in the alveolar wash.The present work is a comparison of the qualitative and quantitative char-