Comparison of the Detection and Quantitative Limits of Hydrocortisone Acetate in Different Chromatographic Conditions in TLC

Dołowy, Małgorzata; Maryszczak, Joanna; Pyka, Alina

doi:10.1080/10826076.2014.907113

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…Hydrocortisone, clioquinol and their binary dosage form are official in British Pharmacopoeia [5] and USP [6]. Several methods for the determination of the drugs have been reported in different dosage forms [7][8][9][10][11][12][13]. Literature survey revealed that HCA and CL binary mixture was determined by HPLC and TLC methods [14,15].…”

Section: Introductionmentioning

confidence: 99%

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

et al. 2021

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Simple and precise spectrophotometric methods for quantitative assay of a mixture of hydrocortisone acetate (HCA) and clioquinol (CL) were developed and validated through different mathematical manipulation pathways. The developed methods utilized ratio spectra for resolving binary mixtures including absorbance subtraction, ratio subtraction coupled with spectrum subtraction, constant multiplication, constant value, and derivative ratio. The proposed methods were proved to be specific by analysing the laboratory-prepared mixtures and were applied for the assay of topical preparation successfully. The methods were validated using ICH guidelines where accuracy, repeatability and intermediate precision were within the acceptable limits. The linearity range was found to be 2-22 for HCA and 1.5-7 µg/mL for CL in all proposed methods and 2-7 µg/mL for HCA and CL in absorbance subtraction method through using a unified regression equation. The findings were statistically evaluated with respect to the official and reported methods, demonstrating that there was no significant difference.

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Section: Introductionmentioning

confidence: 99%

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

et al. 2021

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“…A literature review showed that there were numerous publications on the determination of HCA separately and along with other APIs in cream formulations including pharmacopeial (BP, 2019; USP, 2018), non‐pharmacopeial and various spectrophotometric and thin‐layer chromatography (TLC) methods (Abbas et al, 2017; Adi‐Dako et al, 2017; Dołowy, Kulpińska‐Kucia, & Pyka, 2014; Dołowy, Maryszczak, & Pyka, 2014; Elkady et al, 2017; Harika & Kumar, 2013; Marcio, Elisabete, Rita, & Sheila, 2017; Varaganti, Chengalva, & Gundala, 2019; Widiretani, Luailia, & Indrayanto, 2013). A number of reports have also been published for the determination of FA along with other preservative excipients, APIs and related impurities using other spectrophotometric methods (BP, 2019; Goswami, Gupta, & Jogia, 2013; Lotfy, Salem, Abdelkawy, & Samir, 2015; Sudhakar, Sharma, & Shrivastava, 2015).…”

Section: Introductionmentioning

confidence: 99%

Analytical method development and determination of hydrocortisone acetate and fusidic acid simultaneously in cream formulation, by reversed‐phase HPLC

Rasool

Kanwal

Waseem

et al. 2020

Biomedical Chromatography

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In this study, an accurate, simple, economical, precise and reproducible reversed‐phase HPLC method was developed for the estimation of fusidic acid and hydrocortisone acetate, according to the International Conference on Harmonization guidelines, in a cream formulation. Chromatographic separation was achieved by isocratic elution, on a Shimadzu reversed‐phased high‐pressure liquid chromatography instrument, equipped with a C18 column (150 × 4.6 mm, 5 μm) and UV detector at 225 nm wavelength, using acetonitrile and 0.05% trifluoroacetic acid (60:40), as a mobile phase and diluent, at flow rate 2 ml/min and an injection volume of 20 μl. The calibration curves were acquired with concentration range 80–120% and mean percentage recoveries for hydrocortisone acetate and fusidic acid were 100.14 and 100.81%, respectively. The limits of detection was obtained as 6.0667 and 6.807 μm ml−1 and the limits of quantification were 20.204 and 20.628 μm ml−1 for hydrocortisone acetate and fusidic acid, respectively. All of the validation parameters were within the acceptance criteria, as per International Conference on Harmonization requirements, for hydrocortisone acetate and fusidic acid. This method was found to be validated, simple, rapid and applicable for the simultaneous estimation of hydrocortisone acetate and fusidic acid by reversed‐phased high‐pressure liquid chromatography, for routine analytical testing in quality control, with a run time of 8 min.

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“…However, none of them is particularly dedicated to a TLC-densitometric method. Based on our previous study referring to the estimations of LODs and LOQs of some steroids, e.g., hydrocortisone acetate and spironolactone [22,23], by TLC-densitometry under different chromatographic conditions and using the calculation procedure recommended by the International Conference on Harmonisation (ICH) guideline as well as by the Konieczka and Namiesnik report [20,21], the current work is aimed at finding chromatographic conditions that can be useful to determine ibuprofen in a wide range of concentrations including the lowest one. In addition to this, the influence of the type of plates and mobile phase composition on the LOD and LOQ of ibuprofen is accurately described.…”

Section: Introductionmentioning

confidence: 99%

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

2020

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Ibuprofen is one of the best-known nonsteroidal anti-inflammatory and analgesic drugs. Following the previous work, the current study is focused on estimating the effect of different chromatographic conditions on the sensitivity of thin-layer chromatography in combination with UV densitometry, i.e., the detection and quantification of ibuprofen in a wide range of its concentrations including the lowest limits of detection (LOD) and quantification (LOQ). For this purpose, a reliable and easy-to-use calculation procedure for LOD and LOQ determination is presented in this work. In addition, the impact of type plates and mobile phase composition on the LOD and LOQ, respectively, of this active substance is accurately described. The results of detection and the quantification level of ibuprofen obtained under applied chromatographic conditions confirmed the utility of silica gel plates as well as silica gel bonded phases (i.e., reversed-phase (RP) plates) in the thin-layer chromatography (TLC)-densitometric analysis of ibuprofen at a low level, i.e., from several nanograms (below one microgram) to a few micrograms/spot. Among all chromatographic systems tested, the best are those consisting of silica gel 60F254 plates with concentrating zone (1.05583) and the mixture of n-hexane:ethyl acetate:glacial acetic acid in ratio 36:12:2 (v/v/v) as well as RP plates, i.e., RP-18F254 aluminum plates developed with methanol:water in volume composition 40:10 (v/v). These chromatographic systems allowed quantifying ibuprofen in the amount of 0.229 µg/spot and 0.228 µg/spot, which is less than 1 µg/spot. It can be stated that different chromatographic systems in combination with UV densitometric scanning at 224 nm proposed in this work can be successfully applied for the cost-effective and sensitive determination of ibuprofen as a widely used drug component as well as a residual in domestic wastewater. It was found that the modification of silica gel as well as the layer thickness of unmodified or modified silica gel 60 can influence the quality of chromatograms and the detection/quantification of ibuprofen in both normal phase (NP) and RP systems. Therefore, to obtain the best possible LOD and LOQ values of ibuprofen with precoated layers, suitable mobile phase and chromatographic plates are required.

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Comparison of the Detection and Quantitative Limits of Hydrocortisone Acetate in Different Chromatographic Conditions in TLC

Cited by 11 publications

References 16 publications

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

Analytical method development and determination of hydrocortisone acetate and fusidic acid simultaneously in cream formulation, by reversed‐phase HPLC

Estimating Limits of Detection and Quantification of Ibuprofen by TLC-Densitometry at Different Chromatographic Conditions

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