Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats

Bertinchant, Jean-Pierre; Robert, E; Polge, Anne; Marty-Double, C; Fabbro‐Peray, Pascale; Poirey, Sophie; Aya, G.; Juan, Jean-Marie; Ledermann, Bertrand; Coussaye, J.-E. de La; Dauzat, M.

doi:10.1016/s0009-8981(00)00223-0

Cited by 101 publications

(92 citation statements)

References 28 publications

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“…1). This finding is consistent with previous results on the early myocardial damage induced by isoproterenol [1,9]. There were no differences in the degree of the change among the dose levels, unlike the results on the clinicopathological parameters described below.…”

supporting

confidence: 93%

“…In addition, different parameters are sometimes employed in toxicity studies, so knowledge regarding such correlation would be valuable information for evaluating the toxicity across toxicity studies (i.e., bridging data between toxicity studies). We have therefore investigated the correlation among clinicopathological parameters of myocardial damage in rats treated with isoproterenol, which is a typical compound for inducing myocardial damage [1,9].…”

mentioning

confidence: 99%

“…Preus et al [9] reported the usefulness of LDH-1, LDH-2, and CK-MB, while various other authors reported troponin I and/or troponin T as markers for myocardial damage in rats [1,2,4,5,8,12]. Recently, the International Life Science Institute (ILSI) / Health Environmental Science Institute (HESI) have undertaken a comprehensive study focusing on the influence of methodological differences regarding troponin I detection in diluted samples of serum from rats treated with isoproterenol.…”

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confidence: 99%

“…Based on the results given in previous reports [1,4], rats were subcutaneously administered with isoproterenol [(-)-isoproterenol hydrochloride, Sigma (St. Louis, MO)] at a dose of 0 (saline, 5 ml/kg), 0.04, 0.4 or 4 mg/kg (n=3-4 per group). Blood was collected from an abdominal vein into tubes containing heparin or a coagulation accelerator at 4 h after the dosing under anesthesia by isoflurane inhalation.…”

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Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

et al. 2007

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Abstract:The correlation among clinicopathological parameters of myocardial damage was investigated in rats administered a single subcutaneous dose of isoproterenol at 0 (saline), 0.04, 0.4 and 4 mg/kg. Total lactate dehydrogenase (LDH), creatine kinase (CK), and their isoenzymes (LDH-1, LDH-2 and CK-MB), as well as troponin I and tropinin T, were measured 4 h after the administration of the drug. Troponin I was determined by a chemiluminescence method using Bayer Centaur and DPC Immulyze, as well as by ELISA. Troponin T was assayed semi-quantitatively using Trop T Sensitive. A high correlation was found among LDH isoenzymes, troponin I (Centaur) and troponin T. The present result provides a baseline for interpreting changes in the different parameters of myocardial damage assayed by different methods in toxicity studies.

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confidence: 99%

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Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

et al. 2007

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“…The medians of the troponin I concentrations increased above the cut-off level 2 hours after substance administration. This time interval was also observed in isoproterenol-induced cardiac injury in rats (Bertinchant, 2000). This was a considerably earlier increase as after an acute myocardial infarction in humans.…”

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confidence: 67%

Short-term increase of serum troponin I and serum heart-type fatty acid-binding protein (H-FABP) in dogs following administration of formoterol

Strauss¹,

Wöhrmann²,

Frank³

et al. 2010

Experimental and Toxicologic Pathology

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et al.. Short-term increase of serum troponin I and serum heart-type fatty acid-binding protein (H-FABP) in dogs following administration of formoterol. Experimental and Toxicologic Pathology, Elsevier, 2010, 62 (4) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d m a n u s c r i p t -2 - AbstractIn this paper, changes in serum levels of the cardiac biomarkers troponin I and the heart-type fatty acid-binding protein (H-FABP) following administration of a longacting ȕ 2 -sympathicomimeticum (LABA = long-acting beta-agonist) to dogs were measured.We measured troponin I in dogs in a four week repeated-dose study with inhalative administration of formoterol (13 μg/kg/d) and a glucocorticoid/formoterol combination (143/16 μg/kg/d). The medians of troponin I increased within three days in both groups, far beyond the cut-off level (0.1 μg/L), but returned to baseline levels on study day 9. The increase was more pronounced in the formoterol-only group (3.29 μg/L) compared to the glucocorticoid/formoterol combination group (1.32 μg/L).In a second study, we measured serum troponin I as well as serum H-FABP levels in several samples over seven days in dogs, receiving a single inhalative dose of a glucocorticoid/formoterol combination (120/12 μg/kg/d). The median of the troponin I concentration increased above the cut-off level within two hours, that of H-FABP within four hours. The medians of both parameters were temporarily above the cut-off levels even on study day 7.Both studies were conducted according to national animal welfare guidelines.To our knowledge, this is the first report which shows a corresponding increase of troponin I and H-FABP in dogs treated with formoterol. Both parameters are more sensitive to detect a drug-induced cardiac injury compared to total LDH, total CK as well as CK MB activity. However, it is recommended to take at least three blood samples per day to assess a temporary increase of troponin I.A c c e p t e d m a n u s c r i p t -3 -

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RETRACTED: The cardioprotective effects of a combination of quercetin and α‐tocopherol on isoproterenol‐induced myocardial infarcted rats

Punithavathi

Prince

2011

J Biochem & Molecular Tox

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The present study aims to evaluate the combined protective effects of quercetin and α-tocopherol on isoproterenol-treated myocardial infarcted rats. Male albino Wistar rats were pretreated with a combination of quercetin (10 mg/kg) and α-tocopherol (10 mg/kg) daily for 14 days. After the pretreatment, rats were injected isoproterenol (100 mg/kg) to induce myocardial infarction. Isoproterenol-treated rats showed increased levels of serum troponins and increased intensities of serum lactate dehydrogenase-1 and -2 isoenzyme bands. Isoproterenol treatment also showed significant decreased levels of antioxidant system and significant increased levels of plasma lipid peroxidation, plasma uric acid, and the heart calcium. Furthermore, isoproterenol-treated rat's electrocardiogram showed elevated ST segments. Combined pretreatment with quercetin and α-tocopherol normalized all the biochemical parameters and minimized the alterations in electrocardiogram. Histopathology of myocardium also confirmed the cardioprotective effects of quercetin and α-tocopherol. In vitro studies confirmed the mechanism of action of quercetin and α-tocopherol. Thus, quercetin and α-tocopherol exhibited cardioprotective effects against isoproterenol-induced cardiotoxicity due to their scavenging free radicals, improving antioxidants and maintaining Ca(2+) levels. Our study also showed that combined pretreatment (quercetin and α-tocopherol) was highly effective than single pretreatment (quercetin or α-tocopherol).

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Comparison of the diagnostic value of cardiac troponin I and T determinations for detecting early myocardial damage and the relationship with histological findings after isoprenaline-induced cardiac injury in rats

Cited by 101 publications

References 28 publications

Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

Correlation among Clinicopathological Parameters of Myocardial Damage in Rats Treated with Isoproterenol

Short-term increase of serum troponin I and serum heart-type fatty acid-binding protein (H-FABP) in dogs following administration of formoterol

RETRACTED: The cardioprotective effects of a combination of quercetin and α‐tocopherol on isoproterenol‐induced myocardial infarcted rats

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