Comparison of the Effects of Ophthalmic Solutions on Human Corneal Epithelial Cells Using Fluorescent Dyes

Xu, Mengyue; Sivak, J. G.; McCanna, David J.

doi:10.1089/jop.2013.0002

Cited by 28 publications

(21 citation statements)

References 36 publications

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“…Fluorescein stains intercellular spaces in areas of tight junction disruption and will permeate into cells with damaged cell membranes. 38 Sloughing of the superficial dead or damaged cells after the low humidity stress at visit 4 may have caused a restriction of fluorescein movement into or around the underlying healthy cells, resulting in less staining.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dry Eye Therapies on Environmentally Induced Ocular Surface Disease

Moore¹,

Paiva²,

Pflugfelder³

2015

American Journal of Ophthalmology

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PURPOSE To evaluate the effectiveness of artificial tears and corticosteroids on mitigating the acute ocular surface response to low humidity environments. DESIGN Single-group, crossover clinical trial. METHODS Twenty subjects with aqueous deficient dry eye were enrolled. Subjects meeting inclusion criteria at visit 1 and were exposed to a baseline 90-minute low humidity environment at visit 2. They then used artificial tears for 2 weeks prior to low humidity exposure at visit 3, followed by 0.1% dexamethasone for two weeks prior to the final low humidity exposure at visit 4. Outcome measures included corneal and conjunctival staining, blink rate and irritation symptoms before and after each low humidity exposure. Digital polymerase chain reaction (PCR) was performed to measure HLA-DR RNA transcripts in conjunctival cells taken by impression cytology at each visit. RESULTS There was significantly less corneal and conjunctival epitheliopathy after the low humidity exposure at visit 4 compared to after the low humidity exposure at visit 3 (p= 0.003). Subjects reported significantly less eye irritation during the low humidity exposure after using the dexamethasone (visit 4) compared to artificial tears (visit 3) (p=0.01). HLA-DR transcripts significantly decreased after the stress at visit 4 (post dexamethasone) compared to visit 2. CONCLUSION Our study demonstrates corticosteroid eye drops mitigate the acute adverse effects of an experimental low humidity challenge, likely due to suppression of stress-activated inflammatory pathways. While extended use of corticosteroids is not indicated, other anti-inflammatory therapies with activity against stress-activated pathways may prove as effective.

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Section: Discussionmentioning

confidence: 99%

Effects of Dry Eye Therapies on Environmentally Induced Ocular Surface Disease

Moore¹,

Paiva²,

Pflugfelder³

2015

American Journal of Ophthalmology

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“…If lagophthalmos is present, even during blink, then ocular lubrication is essential and should be maintained long term (LoE 4, GoR D). Based on several studies on dry eye, preservative‐free topical lubrication is strongly recommended for patients who require long‐term eye‐drop administration . Examples of lubricants include carboxymethylcellulose 0.5–1%, carmellose sodium, or hyaluronic acid and petrolatum ointment at night if nocturnal lagophthalmos exists .…”

Section: Complicationsmentioning

confidence: 99%

Management of congenital ichthyoses: European guidelines of care, part two

et al. 2018

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These guidelines for the management of congenital ichthyoses have been developed by a multidisciplinary group of European experts following a systematic review of the current literature, an expert conference held in Toulouse in 2016, and a consensus on the discussions. These guidelines summarize evidence and expert-based recommendations and intend to help clinicians with the management of these rare and often complex diseases. These guidelines comprise two sections. This is part two, covering the management of complications and the particularities of some forms of congenital ichthyosis.

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“…As discussed in the ‘Aqueous deficiency treatment options’ section, because of concerns regarding the effects of preservatives on the ocular surface [92], the authors recommend a nonpreserved formulation for patients using artificial tear supplements in excess of four times per day [92–95]. …”

Section: Treatment Options For Dysfunctional Tear Syndromementioning

confidence: 99%

“…The authors recommend that patients with goblet cell loss and/or mucin deficiency use preservative-free formulations to avoid further damage to the conjunctival epithelium, particularly if tear supplements are used more than four times per day [92–95]. …”

Section: Treatment Options For Dysfunctional Tear Syndromementioning

confidence: 99%

Dysfunctional tear syndrome

Milner

Beckman

Luchs

et al. 2017

Current Opinion in Ophthalmology

168

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Dysfunctional tear syndrome (DTS) is a common and complex condition affecting the ocular surface. The health and normal functioning of the ocular surface is dependent on a stable and sufficient tear film. Clinician awareness of conditions affecting the ocular surface has increased in recent years because of expanded research and the publication of diagnosis and treatment guidelines pertaining to disorders resulting in DTS, including the Delphi panel treatment recommendations for DTS (2006), the International Dry Eye Workshop (DEWS) (2007), the Meibomian Gland Dysfunction (MGD) Workshop (2011), and the updated Preferred Practice Pattern guidelines from the American Academy of Ophthalmology pertaining to dry eye and blepharitis (2013). Since the publication of the existing guidelines, new diagnostic techniques and treatment options that provide an opportunity for better management of patients have become available. Clinicians are now able to access a wealth of information that can help them obtain a differential diagnosis and treatment approach for patients presenting with DTS. This review provides a practical and directed approach to the diagnosis and treatment of patients with DTS, emphasizing treatment that is tailored to the specific disease subtype as well as the severity of the condition.

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Comparison of the Effects of Ophthalmic Solutions on Human Corneal Epithelial Cells Using Fluorescent Dyes

Cited by 28 publications

References 36 publications

Effects of Dry Eye Therapies on Environmentally Induced Ocular Surface Disease

Effects of Dry Eye Therapies on Environmentally Induced Ocular Surface Disease

Management of congenital ichthyoses: European guidelines of care, part two

Dysfunctional tear syndrome

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