Comparison of the Effects of Endotracheal Intubation of Wistar Rats Using the Conventional Technique vs. a New Modified Technique Using a 3D Mouth-Piece

Bravo-Reyna, Carlos; López-Gómez, O.; Zentella, Alejandro; Guerra-Mora, José Raúl; Torres‐Villalobos, Gonzalo; Perales-Caldera, E.; Frías-Guillén, Jessica; Granados, Jesús Benítez

doi:10.1080/08941939.2020.1736217

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…When animals are deeply anesthetized or sedated, this method provides stable and reliable respiration to rats. Many other methods for rodent intubation have been described in the literature [ 3 , 7 , 8 ].…”

Section: Discussionmentioning

confidence: 99%

Micropipette tip intubation in rats as a replacement for conventional endotracheal tube intubation

Kim

Ryu

Lee

et al. 2023

Arch Craniofac Surg

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Endotracheal intubation is often necessary in the course of animal experiments, especially in craniofacial surgery. However, endotracheal intubation can be a major burden in this context. The authors performed simple and cost-saving method using a 200 μL yellow micropipette tip, and the success of this method was demonstrated by X-ray and autopsy. We used a total of 30 rats. After the rats were fixed with a plaster, the trachea and vocal cords were visualized with the tongue pulled back. Under direct visualization of the vocal cords, a curving micropipette tip was advanced into the trachea. This method can be learned quickly and applied successfully by general experimenters. We successfully intubated all 30 rats without any complications. The success rate of micropipette tip intubation was 100%. This procedure was performed by one experimenter within 2 to 3 minutes after induction of anesthesia. We demonstrated its superiority by X-ray and autopsy. Herein, we describe endotracheal intubation of rats using micropipette tips. To the best of our knowledge, this method is novel and represents the simplest and most efficient means of intubation in rats, providing an alternative to conventional endotracheal intubation.

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Section: Discussionmentioning

confidence: 99%

Micropipette tip intubation in rats as a replacement for conventional endotracheal tube intubation

Kim

Ryu

Lee

et al. 2023

Arch Craniofac Surg

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“…Esta técnica se utiliza de forma rutinaria por nuestro equipo de investigación. Las ratas Wistar se sedan e intuban de manera orotraqueal para conectarse a un equipo de ventilación mecánica 23 (Fig. 2 A-D).…”

Section: Inducción De Aneurisma De La Aorta Descendente Por Vía Torác...unclassified

Desarrollo, técnicas y utilidad de modelos animales experimentales de aneurismas aórticos torácicos y abdominales

Bravo-Reyna,

Mejía-Cervantes,

Verduzco-Vázquez

et al. 2024

ACM

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Los aneurismas son una de las entidades clínicas que pueden desarrollarse y afectar la aorta humana. Aunque en la mayoría de los casos tienen un carácter asintomático, estas dilataciones patológicas pueden resultar letales cuando se presentan con ruptura, por lo que el reconocimiento de factores predictores de esta complicación es crucial para evitar muertes. Fisiopatológicamente se han identificado eventos esenciales que ocurren en la pared del vaso, como inflamación, proteólisis, apoptosis del músculo liso, angiogénesis y remodelación. Las grandes especies como porcinos y ovinos han sido de utilidad para el desarrollo y evaluación del desempeño de dispositivos endovasculares en la aorta, así como la remodelación; con el advenimiento y disposición de estas técnicas mínimamente invasivas para su reparación existe una menor disponibilidad de tejido aórtico para el análisis molecular, celular e histopatológico, por lo que en las últimas tres décadas se han propuesto e introducido distintos modelos que han permitido, mediante la inducción focal de estas lesiones, el estudio de los mecanismos fisiopatológicos y posibles biomarcadores de utilidad como dianas diagnósticas y terapéuticas. El presente artículo de revisión aborda tipos de modelos animales disponibles, así como sus aplicaciones, consideraciones, ventajas y limitaciones para el desarrollo de estudios preclínicos y su importancia en el entendimiento de esta patología en la especie humana.

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Experimental Lung Transplantation Related With HIF-1, VEGF, ROS. Assessment of HIF-1α, VEGF, and Reactive Oxygen Species After Competitive Blockade of Chetomin for Lung Transplantation in Rats

Bravo-Reyna,

Zentella,

Ventura-Galleos

et al. 2024

Physiol Res

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Primary graft failure occurs 15 to 30 % of the time after transplantation. Although there have been improvements in preserving the lungs in good condition, there have not been studies on the regulation of transcription factors. Methods: We carried out an experimental study involving lung transplantation to indirectly evaluate reactive oxygen species (ROS) production and VEGF expression by competitive blockade of HIF-1α with chetomin. There were 5 groups: Group-1: Lung blocks were perfused with 0.9 % SSF, immediately harvested, and preserved. Group-2 (I-T): Immediate transplantation and then reperfusion for 1 h. Group-3 (I-R): Lung blocks were harvested and preserved in LPD solution for 6 h and reperfused for 1 h. Group-4 (DMSO): Lung blocks were treated for 4 h with DMSO, preserved for 6 h and transplanted to a receptor treated with DMSO. Group-5 (chetomin): Lung blocks were treated for 4 h with chetomin, preserved for 6 h and transplanted to a receptor treated with chetomin. ROS, mRNA, and protein levels of HIF-1α and EG-VEGF were determined. Results: The DMSO and chetomin groups had significantly lower ROS levels. Compared with those in the I-R group, the chetomin group exhibited the lowest level of HIF-1α. Conclusions: Addition of chetomin to the donor and the receptor results in a significant reduction in HIF-1A, VEGF and ROS.

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Comparison of the Effects of Endotracheal Intubation of Wistar Rats Using the Conventional Technique vs. a New Modified Technique Using a 3D Mouth-Piece

Cited by 3 publications

References 10 publications

Micropipette tip intubation in rats as a replacement for conventional endotracheal tube intubation

Micropipette tip intubation in rats as a replacement for conventional endotracheal tube intubation

Desarrollo, técnicas y utilidad de modelos animales experimentales de aneurismas aórticos torácicos y abdominales

Experimental Lung Transplantation Related With HIF-1, VEGF, ROS. Assessment of HIF-1α, VEGF, and Reactive Oxygen Species After Competitive Blockade of Chetomin for Lung Transplantation in Rats

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