Comparison of the effects of major fatty acids present in the Mediterranean diet (oleic acid, docosahexaenoic acid) and in hydrogenated oils (elaidic acid) on 7-ketocholesterol-induced oxiapoptophagy in microglial BV-2 cells

Debbabi, Meryam; Zarrouk, Amira; Bezine, Maryem; Meddeb, Wiem; Nury, Thomas; Badreddine, Asmaa; Karym, El Mostafa; Sghaier, Randa; Brétillon, Lionel; Guyot, Stéphane; Samadi, Mohammad; Cherkaoui‐Malki, Mustapha; Nasser, Boubker; Mejri, Mondher; Ben-Hammou, Sofien; Hammami, Mohamed; Lizard, Gérard

doi:10.1016/j.chemphyslip.2017.04.002

Cited by 67 publications

(55 citation statements)

References 125 publications

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“…These earlier observations led us to evaluate the ability of argan oils to prevent the toxic effects of 7KC, which is associated with numerous age-related diseases [ 14 ], and has been identified at elevated levels in the brains of patients with Alzheimer’s diseases [ 36 ]. 7KC could contribute to neurodegeneration via its ability to induce numerous side effects associated with neurodegenerescence, including the overproduction of reactive oxygen species, and the induction of mitochondrial, lysosomal and peroxisomal dysfunction, and cell death [ 35 , 54 ]. The ability of argan oil to impair 7KC-induced side effects was compared to that of α-tocopherol used as positive control.…”

Section: Resultsmentioning

confidence: 99%

Argan Oil-Mediated Attenuation of Organelle Dysfunction, Oxidative Stress and Cell Death Induced by 7-Ketocholesterol in Murine Oligodendrocytes 158N

Badreddine

Zarrouk

Karym

et al. 2017

IJMS

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Argan oil is widely used in Morocco in traditional medicine. Its ability to treat cardiovascular diseases is well-established. However, nothing is known about its effects on neurodegenerative diseases, which are often associated with increased oxidative stress leading to lipid peroxidation and the formation of 7-ketocholesterol (7KC) resulting from cholesterol auto-oxidation. As 7KC induces oxidative stress, inflammation and cell death, it is important to identify compounds able to impair its harmful effects. These compounds may be either natural or synthetic molecules or mixtures of molecules such as oils. In this context: (i) the lipid profiles of dietary argan oils from Berkane and Agadir (Morocco) in fatty acids, phytosterols, tocopherols and polyphenols were determined by different chromatographic techniques; and (ii) their anti-oxidant and cytoprotective effects in 158N murine oligodendrocytes cultured with 7KC (25–50 µM; 24 h) without and with argan oil (0.1% v/v) or α-tocopherol (400 µM, positive control) were evaluated with complementary techniques of cellular and molecular biology. Among the unsaturated fatty acids present in argan oils, oleate (C18:1 n-9) and linoleate (C18:1 n-6) were the most abundant; the highest quantities of saturated fatty acids were palmitate (C16:0) and stearate (C18:0). Several phytosterols were found, mainly schottenol and spinasterol (specific to argan oil), cycloartenol, β-amyrin and citrostadienol. α- and γ-tocopherols were also present. Tyrosol and protocatechic acid were the only polyphenols detected. Argan and extra virgin olive oils have many compounds in common, principally oleate and linoleate, and tocopherols. Kit Radicaux Libres (KRL) and ferric reducing antioxidant power (FRAP) tests showed that argan and extra virgin olive oils have anti-oxidant properties. Argan oils were able to attenuate the cytotoxic effects of 7KC on 158N cells: loss of cell adhesion, cell growth inhibition, increased plasma membrane permeability, mitochondrial, peroxisomal and lysosomal dysfunction, and the induction of oxiapoptophagy (OXIdation + APOPTOsis + autoPHAGY). Altogether, our data obtained in 158N oligodendrocytes provide evidence that argan oil is able to counteract the toxic effects of 7KC on nerve cells, thus suggesting that some of its compounds could prevent or mitigate neurodegenerative diseases to the extent that they are able to cross the blood-brain barrier.

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Section: Resultsmentioning

confidence: 99%

Argan Oil-Mediated Attenuation of Organelle Dysfunction, Oxidative Stress and Cell Death Induced by 7-Ketocholesterol in Murine Oligodendrocytes 158N

Badreddine

Zarrouk

Karym

et al. 2017

IJMS

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“…However, the highest content of unsaturated fatty acid docosahexaenoic acid (C22:6n3) was sheep with AA genotype. Docosahexaenoic acid (C22:6n3) and oleic acid (C18:1n9c) were able to reduce the toxic effects of 7-ketocholesterol (7KC), thought to contribute to reduce risk of neurodegenerative disease and age-related diseases (Debbabi et al, 2017). Previous study reported an association of LEPR with fatty acid composition in pigs (Oliveira et al, 2017;Rodriguez et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep

Gunawan

Pramukti

Listyarini

et al. 2019

J. Indonesian Trop. Anim. Agric.

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The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.

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“…These results are in agreement with the cardioprotective effects of DHA widely reported through its anti-inflammatory [ 31 ] and anti-oxidant activities [ 33 – 35 ]. It has been recently reported that DHA, one of the main fatty acids of the Mediterranean diet, attenuates the 7-KC-induced toxic effect on microgial cells [ 81 ]. Based on all this information, human dietary habits deserve important consideration and comsumption of a Mediterranean diet could prevent the consequences of an increased oxidative stress that occurs in diverse physiopathological situations.…”

Section: Discussionmentioning

confidence: 99%

Dietary docosahexaenoic acid supplementation prevents the formation of cholesterol oxidation products in arteries from orchidectomized rats

et al. 2017

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Testosterone deficiency has been correlated with increased cardiovascular diseases, which in turn has been associated with increased oxidative stress. Several studies have considered cholesterol oxidation products (COPs) as oxidative stress biomarkers, since some of them play pro-oxidant and pro-inflammatory roles. We have previously described the cardioprotective effects of a dosahexaenoic acid (DHA) supplemented diet on the aortic and mesenteric artery function of orchidectomized rats. The aim of this study was to investigate whether impaired gonadal function alters the formation of COPs, as well as the potential preventive role of a DHA-supplemented diet on that effect. For this purpose, aortic and mesenteric artery segments obtained from control and orchidectomized rats, fed with a standard or supplemented with DHA, were used. The content of the following COPs: 7α-hydroxycholesterol, 7β-hydroxycholesterol, 7-ketocholesterol, 5,6α-epoxycholesterol, 5,6β-epoxycholesterol, cholestanetriol and 25-hydroxycholesterol, were analyzed by gas chromatography. The results showed that orchidectomy increased the formation of COPs in arteries from orchidectomized rats, which may participate in the orchidectomy-induced structural and functional vascular alterations already reported. The fact that the DHA-supplemented diet prevented the orchidectomy-induced COPs increase confirms the cardiovascular protective actions of DHA, which could be of special relevance in mesenteric arterial bed, since it importantly controls the systemic vascular resistance.

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Comparison of the effects of major fatty acids present in the Mediterranean diet (oleic acid, docosahexaenoic acid) and in hydrogenated oils (elaidic acid) on 7-ketocholesterol-induced oxiapoptophagy in microglial BV-2 cells

Cited by 67 publications

References 125 publications

Argan Oil-Mediated Attenuation of Organelle Dysfunction, Oxidative Stress and Cell Death Induced by 7-Ketocholesterol in Murine Oligodendrocytes 158N

Argan Oil-Mediated Attenuation of Organelle Dysfunction, Oxidative Stress and Cell Death Induced by 7-Ketocholesterol in Murine Oligodendrocytes 158N

Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep

Dietary docosahexaenoic acid supplementation prevents the formation of cholesterol oxidation products in arteries from orchidectomized rats

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