Comparison of the effects of sciatic nerve crush or resection on the proteins of fast axonal transport in rat dorsal root ganglion cell axons

Redshaw, J.D.; Bisby, Mark A.

doi:10.1016/0014-4886(85)90205-5

Cited by 32 publications

(11 citation statements)

References 24 publications

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“…Initial one-dimensional separations of fast-transported proteins in sensory axons from animals treated for 10 days with acrylamide, and injected with L-[~~S]methionine on day 1 1, revealed consistent differences from untreated animal samples only in the 20-30 KDa region of the gel where we had previously detected changes in regenerating nerves (Bisby, 1980;Redshaw and Bisby, 1985). Bis-acrylamide-treated animals showed no differences in labelling pattern from untreated animals.…”

Section: Resultsmentioning

confidence: 62%

Acrylamide Neuropathy: Changes in the Composition of Proteins of Fast Axonal Transport Resemble Those Observed in Regenerating Axons

Bisby

Redshaw

1987

Journal of Neurochemistry

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Proteins conveyed by fast axonal transport along sensory and motor axons of rat sciatic nerve were labelled with L-[35S]methionine and characterized by one- and two-dimensional electrophoresis on polyacrylamide gels, followed by fluorography. Nerves from normal or bis-acrylamide-treated animals were compared with nerves from acrylamide-treated animals and nerves regenerating after a crush axotomy. In both sensory and motor axons significant changes in the pattern of labelled bands on one-dimensional gels occurred after 10 days of acrylamide treatment (50 mg/kg daily, i.p.). These changes resembled those seen in regenerating axons, but were less pronounced. No changes were detectable after shorter periods of treatment, even though the onset of the neuropathy, assessed by a behavioral test, occurred on days 4-6 of treatment. Two-dimensional separations of the labelled proteins revealed increased labelling of growth-associated protein 43 in acrylamide-treated animals, but again this was less pronounced than in regenerating nerves. Acrylamide treatment induces changes in composition of fast-transported protein that are qualitatively similar to those seen after axotomy. Since these changes are not detectable until the neuropathy is advanced, it is unlikely that they are causative factors. Instead, they are most likely a result of the cell body reaction previously observed in acrylamide intoxication, a reaction that resembles that produced by axotomy.

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Section: Resultsmentioning

confidence: 62%

Acrylamide Neuropathy: Changes in the Composition of Proteins of Fast Axonal Transport Resemble Those Observed in Regenerating Axons

Bisby

Redshaw

1987

Journal of Neurochemistry

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“…When DRG neurons are axotomized to elicit axon regeneration in vivo and are subsequently explanted to tissue culture, the cultured neurons uniformly exhibit a rapid regrowth of neurites that can extend for several millimeters in vitro. The elongation rate and lengths of these neurites resembles the regeneration of axons in vivo, which is accompanied by induction of the major palmitoylated growth cone protein, GAP-43 (Basi et al, 1987;Hoffman, 1989;Schreyer and Skene, 1991) and a partial reduction in expression of SNAP-25 (Redshaw and Bisby, 1985). Thus, cultured adult DRG neurons more accurately reflect aspects of axon growth required for de novo pathway formation and nerve regeneration in vivo, and their rapid growth in vitro allows a more rapid and unambiguous quantitation of effects on growth cone function than with PC12 cells.…”

Section: Tm Impairs Axon Regeneration By Primary Neuronsmentioning

confidence: 98%

Novel inhibitory action of tunicamycin homologues suggests a role for dynamic protein fatty acylation in growth cone-mediated neurite extension

Patterson

Skene

1994

The Journal of Cell Biology

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Abstract. In neuronal growth cones, the advancing tips of elongating axons and dendrites, specific protein substrates appear to undergo cycles of posttranslational modification by covalent attachment and removal of long-chain fatty acids. We show here that ongoing fatty acylation can be inhibited selectively by longchain homologues of the antibiotic tunicamycin, a known inhibitor of N-linked glycosylation. Tunicamycin directly inhibits transfer of palmitate to protein in a cell-free system, indicating that tunicamycin inhibition of protein palmitoylation reflects an action of the drug separate from its previously established effects on glycosylation. Tunicamycin treatment of differentiated PC12 cells or dissociated rat sensory neurons, under conditions in which protein palmitoylation is inhibited, produces a prompt cessation of neurite elongation and induces a collapse of neuronal growth cones. These growth cone responses are rapidly reversed by washout of the antibiotic, even in the absence of protein synthesis, or by addition of serum. Two additional lines of evidence suggest that the effects of tunicamycin on growth cones arise from its ability to inhibit protein long-chain acylation, rather than its previously established effects on protein glycosylation and synthesis. The tunicamycin-induced impairment of growth cone function can be reversed by the addition of excess exogenous fatty acid, which reverses the inhibition of protein palmitoylation but has no effect on the inhibition of protein glycosylation. These results suggest an important role for dynamic protein acylation in growth cone-mediated extension of neuronal processes.

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“…2c of Rulli and Wilson (1987). Likewise, studies of other species and other preparations have not revealed qualitative changes resembling np31 (Skene and Willard, 1981;Redshaw and Bisby, 1985;Perry et al, 1987~;Savage et al, 1990). We cannot rule out species differences as a cause for these discrepancies.…”

Section: Discussionmentioning

confidence: 82%

A Fast Axonally Transported Protein of the Frog Sciatic Sensory Axons Undergoes Similar Qualitative Changes During Regeneration In Vitro and In Vivo

Remgård

Ekström

Edström

1991

Journal of Neurochemistry

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The adult frog sciatic sensory neurons have been shown to regenerate in vitro. If a crush injury is made at the beginning of culture, regeneration starts after 3.4 days and proceeds at a rate of approximately 0.8 mm/day for several days. Two-dimensional gel electrophoresis was used to study the patterns of radiolabeled, fast axonally transported proteins during the first 7 days of regeneration. Interest was focused on one protein, referred to as rrp31 (regeneration-related protein 31), which changed in apparent pI from 4.9 to 5.3 when the outgrowth of new fibers started. The change was noticeable 3 days after injury and became prominent during day 5 of culturing. By day 7 the pI changed again, this time toward the original value. The in vitro results were supported by experiments in vivo. In this case the change occurred earlier, with a peak only 3 days after injury, after which the pI decreased. If adenosine at 1 mM was included in the culturing medium, the outgrowth of sensory axons was inhibited in a nontoxic way, and the pI changes of rrp31 were prevented. The temporal nature of the pI changes suggests a role for rrp31 in the initiation of the regeneration process.

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Comparison of the effects of sciatic nerve crush or resection on the proteins of fast axonal transport in rat dorsal root ganglion cell axons

Cited by 32 publications

References 24 publications

Acrylamide Neuropathy: Changes in the Composition of Proteins of Fast Axonal Transport Resemble Those Observed in Regenerating Axons

Acrylamide Neuropathy: Changes in the Composition of Proteins of Fast Axonal Transport Resemble Those Observed in Regenerating Axons

Novel inhibitory action of tunicamycin homologues suggests a role for dynamic protein fatty acylation in growth cone-mediated neurite extension

A Fast Axonally Transported Protein of the Frog Sciatic Sensory Axons Undergoes Similar Qualitative Changes During Regeneration In Vitro and In Vivo

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