Comparison of the Effects of Ultraviolet Light and Purinergic Nerve Stimulation on the Guinea‐pig Taenia Coli

Abstract

The responses of the guinea‐pig taenia coli, urinary bladder and the rabbit portal vein to ultraviolet (u.v.) light were compared to those elicited by purinergic nerve stimulation and exogenous adenosine triphosphate (ATP). In the presence of sodium nitrite, u.v. light between 340‐380 nm produced a maximum relaxation of the taenia coli. The relaxation was reversible and fast in onset. It was unaffected by atropine, guanethidine or low concentrations of phentolamine or propranolol. When the tone was low, the relaxation was usually followed by a ‘rebound contraction’ upon cessation of stimulation. Thus, the response to u.v. light closely resembles the responses to both purinergic nerve stimulation and exogenously applied ATP. U.v. light did not initiate impulses in purinergic nerves since its action was unaffected by tetrodotoxin; nor did it release ATP from nerve terminals (in contrast to its release during purinergic nerve stimulation). The adenosine‐uptake inhibitor, dipyridamole, which potentiates the responses to purinergic nerve stimulation and ATP, did not affect the response to u.v. light. Agents known to alter postjunctional responses to purinergic nerve stimulation and ATP also altered the response to u.v. light. High concentrations of the 2‐substituted imidazoline compounds, antazoline and phentolamine, which antagonize the responses to purinergic nerve stimulation and ATP, reduced the responses to u.v. irradiation. The prostaglandin synthesis inhibitor, indomethacin, which abolishes the ‘rebound contraction’ following stimulation of purinergic nerves, also blocks the ‘rebound ‘contraction’ following u.v. irradiation. Increases in the K+ concentration produced parallel changes in the inhibitory responses to u.v. light and purinergic nerve stimulation. U.v. light produced relaxation and inhibition of spontaneous activity of the rabbit portal vein (relaxed by ATP), but had no effect on the guinea‐pig urinary bladder (contracted by ATP) and ureter (unaffected by ATP). It is suggested that u.v. light is acting on some part of the purinergic receptor complex which is involved in the mediation of inhibitory responses to ATP and purinergic nerve stimulation, and may therefore provide a way of investigating the chemistry of inhibitory purinergic receptors.

Studies on the stereoselectivity of the P2‐purinoceptor

“…Apamin, a potassium channel blocker (Banks, Brown, Burgess, Burnstock, Claret, Cocks & Jenkinson, 1979), noncompetitively antagonizes the P2-mediated inhibition of the guinea-pig taenia coli but does not affect the P2-mediated excitation in the guinea-pig bladder (Shuba & Vladimirova, 1980). In addition, ultraviolet light produces a rapid, reversible and non nerve-mediated relaxation of the guinea-pig taenia coli but has no effect on the guinea-pig bladder (Burnstock & Wong, 1978).…”

Effects of phosphorothioate analogues of ATP, ADP and AMP on guinea‐pig taenia coli and urinary bladder

“…Other differences have also been reported between the taenia coli and bladder; for example, apamin, a potassium channel blocker (Banks et al, 1979) inhibits ATP actions in the taenia but not in the bladder (Shuba & Vladimirova, 1980), and, in addition, ultraviolet light mimics the response of the taenia but not of the bladder, to ATP (Burnstock & Wong, 1978).…”

Purinergic signalling: from discovery to current developments

“…Atropine (1.5 × 10 −7 m ), guanethidine (5 × 10 −6 m ) and sodium nitrite (7.2 × 10 −4 m ) were present. [From Burnstock & Wong (1978), reproduced with kind permission of the Nature Publishing Group.] A , right-hand side shows a comparison of the NANC contractile responses of the guinea-pig bladder strip to intramural nerve stimulation (NS; 5 Hz, 0.2 ms pulse duration and supramaximal voltage) mimicked by exogenous ATP (8.5 μ m ).…”