Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota

Lamari, Faouzi; Castex, Mathieu; Larcher, Thibaut; Ledevin, Mireille; Mazurais, David; Bakhrouf, Amina; Gatesoupe, François-Joël

doi:10.1016/j.aquaculture.2012.11.016

Cited by 48 publications

(44 citation statements)

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“…Newly-hatched sea bass larvae were provided by Aquastream (Ploemeur, France), allotted at 2 dph (day post hatch) in 15 tanks of 35 L, and then reared in the general conditions described elsewhere [40]. The 15 tanks were divided in three groups of five tanks, which were fed the compound diets from 7 dph onwards (Fig.…”

Section: Methodsmentioning

confidence: 99%

The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories

et al. 2016

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BackgroundThe better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with Specific Growth Rate (SGR) and Hypoxia Resistance Time (HRT) in European sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challenge in a common garden approach at the juvenile stage.ResultsA diet deficient in essential fatty acids (LH) lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient diet with high starch supply (HG) reduced SGR to a lesser extent than LH, but it did not affect HRT. In overall average, 94 % of pyrosequencing reads corresponded to Proteobacteria, and the differences in Operational Taxonomy Units (OTUs) composition were mildly significant between experimental groups, mainly due to high individual variability. The highest and the lowest Bray-Curtis indices of intra-group similarity were observed in the two groups fed standard starter diet, and then mixed before the final dietary challenge with fish already exposed to the nutritional deficiency at first feeding (0.60 and 0.42 with diets HG and LH, respectively). Most noticeably, the median percentage of Escherichia-Shigella OTU_1 was less in the group LH with standard starter diet. Disregarding the nutritional history of each individual, strong correlation appeared between (1) OTU richness and SGR, and (2) dominance index and HRT. The two physiological traits correlated also with the relative abundance of distinct OTUs (positive correlations: Pseudomonas sp. OTU_3 and Herbaspirillum sp. OTU_10 with SGR, Paracoccus sp. OTU_4 and Vibrio sp. OTU_7 with HRT; negative correlation: Rhizobium sp. OTU_9 with HRT).ConclusionsIn sea bass, gut microbiota characteristics and physiological traits of individuals are linked together, interfering with nutritional history, and resulting in high variability among individual microbiota. Many samples and tank replicates seem necessary to further investigate the effect of experimental treatments on gut microbiota composition, and to test the hypothesis whether microbiotypes may be delineated in fish.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0885-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories

et al. 2016

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“…Importantly, the ecological relations among gastrointestinal bacteria are responsible for benefits to the immune system [13,14,15,16,17,18,19,20], nutrient metabolism [21], growth [22], stress tolerance [23,24], vertebral column deformities, bone calcification and density [25,26,27,28], development [29,30] and reproduction [31,32,33,34], all deriving from their administration.…”

Section: Introductionmentioning

confidence: 99%

Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes

Miccoli

Gioacchini

Maradonna

et al. 2015

Cell Physiol Biochem

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Background/Aims: Probiotic strains have been recognized to exert important roles in many biological systems, including immune response, growth, development and reproduction. However, to date, no studies have focused either on the relation among probiotics and maternal factors or on probiotics' ability to qualitatively and/or quantitatively modulate maternal transcripts. Methods: In this study, the effects of Lactobacillusrhamnosus administered to parental fish on the control of maternal factors involved in autophagic, apoptotic and dorsalizing processes during zebrafish embryo development were assessed through q-PCRs, WMISH and TUNEL assay. Results: The results we obtained show that probiotic induced significant changes in both maternal and zygotic mRNA levels involved in embryo development. The maternal autophagy-regulating genes herein investigated -ambra1a, ambra1b, beclin, lc3-, as well as those involved in the apoptotic process -caspase3, bcl2, bax- were modulated in disfavor and favor of the treated group, respectively. Also, the key transcripts ruling the dorsalizing process -goosecoid and chordin- were subject to a significant regulation of their gene expression. Conclusion: The results we acquired demonstrated that parentally administered Lactobacillusrhamnosus is able to modulate important physiological processes involved in zebrafish embryo development.

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“…The method allowed us to discriminate the bacterial phylotypes after their partial sequences of 16S and 26S rRNA for bacteria and fungi, respectively. The sequences could not be systematically read, but some bands were extracted from the DGGE gels for bacterial sequencing, when feasible (Lamari et al, 2013). …”

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“…The intestinal sections from the juveniles were also placed in sterile Petri dishes on ice after thawing, to squeeze out the gut mucosae and the associated microbiota with a metallic spatula. The samples were immediately collected with sterile tweezers, and immersed in Extract-All ® (Eurobio) for RNA extraction and subsequent RT-PCR-DGGE, as described for bacterial profiling by Lamari et al (Lamari et al, 2013). Fungi were analysed using the primers NL1GC and LS2 (Verdugo Valdez et al, 2011) with the same PCR conditions as for bacteria.…”

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High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

Geurden

Mennigen

Plagnes-Juan

et al. 2014

Journal of Experimental Biology

103

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Based on the concept of nutritional programming in mammals, we tested whether an acute hyperglucidic-hypoproteic stimulus during first feeding could induce long-term changes in nutrient metabolism in rainbow trout. Trout alevins received during the five first days of exogenous feeding either a hyperglucidic (40% gelatinized starch + 20% glucose) and hypoproteic (20%) diet (VLP diet) or a high-protein (60%) glucose-free diet (HP diet, control). Following a common 105-day period on a commercial diet, both groups were then challenged (65 days) with a carbohydrate-rich diet (28%). Short-and long-term effects of the early stimuli were evaluated in terms of metabolic marker gene expressions and intestinal microbiota as initial gut colonisation is essential for regulating the development of the digestive system. In whole alevins (short term), diet VLP relative to HP rapidly increased gene expressions of glycolytic enzymes, while those involved in gluconeogenesis and amino acid catabolism decreased. However, none of these genes showed persistent molecular adaptation in the liver of challenged juveniles (long term). By contrast, muscle of challenged juveniles subjected previously to the VLP stimulus displayed downregulated expression of markers of glycolysis and glucose transport (not seen in the short term). These fish also had higher plasma glucose (9 h postprandial), suggesting impaired glucose homeostasis induced by the early stimulus. The early stimulus did not modify the expression of the analysed metabolism-related microRNAs, but had short-and long-term effects on intestinal fungi (not bacteria) profiles. In summary, our data show that a short hyperglucidic-hypoproteic stimulus during early life may have a long-term influence on muscle glucose metabolism and intestinal microbiota in trout. KEY WORDS: Nutritional programming, Rainbow trout, Carbohydrates, Protein, Metabolism INTRODUCTIONPrenatal or early nutritional neonatal events exerted during critical developmental windows may result in permanent changes in postnatal growth potential, health and metabolic status in mammals (Burdge and Lillycrop, 2010;Lucas, 1998;Patel and Srinivasan, 2002;Patel et al., 2009;Metges et al., 2014;Duque-Guimarães and Ozanne, 2013;Devaskar and Thamotharan, 2007). It has been RESEARCH ARTICLE 1 INRA, UR1067 Nutrition Metabolism and Aquaculture, F-64310 Saint-Pée-surNivelle, France. suggested that this process of developmental plasticity utilizes the early nutritional cues to prepare individual phenotypes to better match the predicted future nutritional environment (Gluckman et al., 2005). Possible biological mechanisms for 'imprinting' the nutritional event until adulthood in mammalian vertebrates comprise adaptive changes in gene expression pattern or cellular phenotype (epigenetic phenomenon), nutrient-sensitive signalling pathways and adaptive clonal selection, which may be transmitted to future offspring (Lucas, 1998;Symonds et al., 2009;Waterland and Jirtle, 2003;Gut and Verdin, 2013). Experimental data on the concept...

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Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota

Abstract: Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota

Cited by 48 publications

References 30 publications

The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories

The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories

Beneficial Bacteria Affect Danio rerio Development by the Modulation of Maternal Factors Involved in Autophagic, Apoptotic and Dorsalizing Processes

High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

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