Comparison of the efficiency and toxicity of sonoporation with branched polyethylenimine-mediated gene transfection in various cultured cell lines

Yoon, Chang Shin; Jung, Hwa Jin; Kim, Tae Kyoung; Kwon, Min Jeong; Kim, Mi K.; Lee, Minhyung; Koh, Kyung Soo; Rhee, Byung Doo; Park, Jeong H.

doi:10.1080/10611860802470549

Cited by 11 publications

(4 citation statements)

References 20 publications

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“…Our findings are in general agreement with earlier work in this field, indicating that transient physical disruption of cell membranes by local ultrasound-induced cavitation is not permanently injurious to the target cells. 31 For clinical applications requiring extended or lifelong gene expression, this is a critical consideration as cells comprising the salivary gland do not significantly regenerate and divide in the adult, and thus preservation of existing salivary gland parenchyma is a paramount consideration when re-dosing of gene therapy is necessary. Practical application of this technology to the clinical practice of dental medicine will need to incorporate more sophisticated plasmid vectors to achieve a maximal interval between gene transfer 'boosters'.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound-assisted non-viral gene transfer to the salivary glands

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Ultrasound-assisted non-viral gene transfer to the salivary glands

et al. 2010

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“…In nonviral gene delivery, although PEI-mediated transfection has been reported as one of the most effective methods, the potential cytotoxicity limits its wide-spread applications [2]. Sonoporation is most intriguing as a simple, non-invasive gene delivery technique, which is enabled by the simple addition of plasmids to cell suspension.…”

Section: Discussionmentioning

confidence: 99%

“…Gene delivery of viral vectors can be effective method, but the safety concerns are still concerned in the clinical applications [2]. Nonviral vectors or naked plasmids are much safer, but the transfection efficiencies are too low to be of clinical value [3].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of survivin gene downregulation and cell apoptosis by a novel combination: liposome microbubbles and ultrasound exposure

et al. 2009

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Ultrasound-mediated microbubble destruction (sonoporation) is an efficient and safe nonviral technique for gene delivery. In the present work, we hypothesized that short hairpin RNA (shRNA) interference therapy targeting human Survivin gene could be transfected by the novel combination of ultrasound exposure (USE) and liposome microbubbles (LM). ShRNA vectors targeting Survivin were constructed and transfected under USE and LM conditions. The optimal transfection efficiency and cell injury were compared with those of polyethylenimine (PEI)-mediated transfection in different cancer cell lines (HeLa, HepG2, Ishikawa, MCF-7, and B16-F10). The effects of gene downregulation and cell apoptosis were further investigated. The results indicated that P + USE + LM group could significantly increase the gene expression as compared with plasmid group, plasmid + USE group, plasmid + LM group (P < 0.001). The transfection efficiency of the novel combination was nearly equal to PEI-mediated transfection in some cancer cell lines while the cell viability did not decrease markedly. Reverse transcription-polymerase chain reaction (RT-PCR) and western blot analysis also confirmed that Survivin mRNA and protein expression could be knocked down significantly by shRNA transfection under USE and LM condition (P < 0.001). This is the first study to verify the role of shRNA therapy in vitro with novel combination of USE and LM. We concluded that this nonviral technique would be valuable in the gene transfection of shRNA and Survivin gene downregulation would lead to apparent cell apoptosis.

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“…Combination with PEI has recently been tested for enhancing the efficiency of sonoporation in vitro. A cationic branched polyethylenimine (BPEI) polymer was used to condense pDNA containing the cDNA expressing human VEGF 165 (pVEGF) (40). pVEGF+BPEI complexes were transfected into CHO, HEK293, or NIH3T3 cells using Sonovue MBbased sonoporation.…”

Section: Other Nucleic Acid Sonoporation Gene Therapy 421 Sonothermentioning

confidence: 99%

Advances in sonoporation strategies for cancer

Figueiredo¹

2012

Front Biosci

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We will focus on the therapeutic applications of ultrasound (US) for gene transfection or 'sonoporation'. Sonoporation therapy or 'sonotherapy' is an emerging physical method for delivering drugs and/or nucleic acids for treating cancer. Because of its non-invasive nature, sonotherapy has the potential to be competitive with other treatment delivery methods such as viruses or lipofection. For nucleic acid delivery, sonoporation in the presence of microbubbles (MB) significantly enhances transfection efficiency. Sonoporation is an ideal means of delivering pDNA, and it has a similar efficiency as electroporation or other physical gene therapy techniques, with potentially fewer side effects. Typically, nonphysical means of gene delivery have suffered from lower efficiencies compared to viral vectors, however, several studies suggest that sonoporation pDNA delivery could be a simple and inexpensive method that only requires a plasmid, MB, and a sonoporation device. Sonoporation could also be used to target MB to certain cells/tissues, delivering localized therapies. Using high-performance probes, more precise and safer sonoporation treatments will be developed, and newer therapeutic prospects will be realized.

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Comparison of the efficiency and toxicity of sonoporation with branched polyethylenimine-mediated gene transfection in various cultured cell lines

Cited by 11 publications

References 20 publications

Ultrasound-assisted non-viral gene transfer to the salivary glands

Ultrasound-assisted non-viral gene transfer to the salivary glands

Enhancement of survivin gene downregulation and cell apoptosis by a novel combination: liposome microbubbles and ultrasound exposure

Advances in sonoporation strategies for cancer

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