Comparison of the Efficiency of Systemic and Local Cell Therapy with Human Umbilical Cord Blood Mononuclear Cells in Rats with Severe Spinal Cord Injury

Severe Spinal Cord Injury in Rats Induces Chronic Changes in the Spinal Cord and Cerebral Cortex Metabolism, Adjusted by Thiamine That Improves Locomotor Performance

“…Manual massage of the abdominal wall was carried out to empty the bladder until self-urination was restored. The white matter damage in our experiments was characterized in previous studies (Ryabov et al, 2014;Ryabov et al, 2020;Smirnov et al, 2020). Shamoperated animals were subjected to laminectomy (LE) at the T9 vertebra without affecting the dura matter.…”

“…7 mm each) was taken for the analyses (Smirnov et al, 2020). Taking into account the earlier studies on the spinal cord changes in the traumatized area, the contribution of the scar tissue was estimated to be within 20% of the total spinal cord tissue taken for analysis (Ryabov et al, 2014;Ryabov et al, 2020;Smirnov et al, 2020). The cerebral hemispheres (further called the cortex) were separated from other brain parts.…”

Therapeutic effect of umbilical cord blood cells on spinal cord injury

“…The results showed that the motor recovery was good, and the motor function of the hind limbs improved obviously, which recovered from the two‐joint motor level to the three‐joint motor level. 11 , 19 , 20 Moreover, transplantation of UCB‐CD34+ significantly improved BBB and Tarlov scores, promoted motor function recovery, and improved the survival rate. 10 Platelet‐rich plasma from cord blood (UCB‐PRP), which includes cord blood cells and plasma, can also restore motor function when used alone as assessed from the BBB score.…”

“…Besides, the BBB size on injection of human UBC MCs was evaluated using the stress test and the open site test. The results showed that the motor recovery was good, and the motor function of the hind limbs improved obviously, which recovered from the two‐joint motor level to the three‐joint motor level 11,19,20 . Moreover, transplantation of UCB‐CD34+ significantly improved BBB and Tarlov scores, promoted motor function recovery, and improved the survival rate 10 .…”

“…Combined treatment is becoming increasingly more important and necessary, for example, UCBs combined with gene transfect and electric stimulation therapy for treatment of SCI. of UCBs 1-6 weeks 9,10,12 Behavioral improvement Effective 9,11,14,15,[17][18][19][20][21][22][23][24][25][26]38 Effective 27,28 Morphological changes Spinal infarct ↓ 9,15,17,23,25,29,30 Myelinated fibers ↑ 15,22,29,30,34,35 ↑ 31 Axons grow ↑ [24][25][26]33,34,36 Fibroblast numbers ↓ 12,34,36 White matter space…”