Comparison of the Efficiency to One-Step and Two-Step Anther Culture in Rice (Oryza sativa L)

Abstract

Anther culture method has commonly been performed two-step culture of callus induction during the plant regeneration stage. Two-step culture was generally required to a much longer duration of culture for regeneration. There are still needed to be solved the disadvantage of the two-step culture technique. Anther of F1 plants derived from Drimi2 *3 /AR1 of new plant type and improved rice cultivars were compared with method efficiency using one-step and two-step culture method. The results showed that one-step culture was more effective than two-step culture. Callus induction averaged 8.2% for one-step culture and 6.1% for two-step culture. The proportion of plants that survived after colchicine treatment was similar as 17.5% for one-step culture and 16.7% for two-step culture. However, there was a highly significant difference when results of plant regeneration were compared. Frequency of regenerated plant averaged 13.6% for one-step culture, which was 7.1% higher than two-step culture (6.5%). These results provide valuable data that may be used to establish an efficient plant regeneration system for rice cultures.

Assessment of the modes of pollen dispersal of Vernonia amygdalina Del. and Vernonia calvoana Hook

“…on the flower lifespan, exact stage and time (hours of the day) of pollen release, stigma receptivity, proper pollen storage and germination conditions (Monteiro et al, 2015) are recommended. These considerations are valuable for eventual establishment of an efficient regeneration system through classical breeding or micropropagation (Min et al, 2015) for Vernonia spp. cultivation.…”