Comparison of the fertility of cryopreserved stallion spermatozoa with sperm motion analyses, flow cytometric evaluation, and zona-free hamster oocyte penetration

Wilhelm, Kyle; Graham, J. K.; Squires, E.L.

doi:10.1016/0093-691x(96)00209-9

Cited by 67 publications

(30 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The other motility parameters read by the CEROS analyser showed that slowly frozen spermatozoa had a lower velocity (VAP and VSL). The significance of this result is not clear, although potentially negative, since significant positive correlations were observed between VSL, VAP and fertility in other species [23]; spermatozoa frozen in the biological freezer also exhibited lower lateral head displacement and curvilinear velocity. These parameters increase during the capacitation process and a precocious capacitation could lead to a premature acrosome reaction and then reduce the life span and fertility of spermatozoa [24].…”

Section: Discussionmentioning

confidence: 81%

Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing

Rota

Martini

et al. 2005

Reprod. Nutr. Dev.

View full text Add to dashboard Cite

-Three ejaculates were collected from each of five dogs. After initial evaluation, the spermrich fractions were diluted to 100 × 10 6 spermatozoa·mL -1 in two steps with an egg yolk-TRIS extender containing a final concentration of 5% glycerol and 0.5% Equex STM paste. Half of the 0.5 mL straws obtained from each ejaculate were frozen on nitrogen vapours (4 cm above the liquid surface) ("rapid freezing"), while the other half was frozen in a biological freezer at a rate of 0.5°C·min -1 between 5 °C and -10 °C and of 8 °C·min -1 between -10 °C and -60 °C, followed by immersion in liquid nitrogen ("slow freezing"). After an average storage of 30 days, the straws were thawed in a water-bath at 37 °C for 1 min. Progressive motility was subjectively estimated hourly for 8 h on semen incubated at 38 °C. Immediately after thawing and after 2 h of incubation, motility parameters were also measured by a motility analyser. Sperm membrane function and chromatin stability were assessed immediately post-thaw, using the hypo-osmotic swelling test and acridine orange staining, respectively. Slow freezing significantly improved total post-thaw motility, which showed a slower decline over time, although spermatozoal average path and straight line velocity were lower compared to the fast rate. Also the number of intact membrane spermatozoa was significantly higher in slow-frozen samples while the proportion of spermatozoa with single-stranded DNA was minimal after both freezing procedures. dog / semen / cryopreservation / freezing rate

show abstract

Section: Discussionmentioning

confidence: 81%

Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing

Rota

Martini

et al. 2005

Reprod. Nutr. Dev.

View full text Add to dashboard Cite

show abstract

“…The resulting crystals were stored at 22 • C until further use. A working solution of CLC was prepared by adding 50 mg of CLC to 1 ml of a 37 • C hepes buffered saline solution (S-MEDIUM; Wilhelm et al, 1996). This CnLC and CLC working solution was vigorously vortexed prior to the removal of any aliquot.…”

Section: Cyclodextrins Preparationmentioning

confidence: 99%

Cholestanol-loaded-cyclodextrin improves the quality of stallion spermatozoa after cryopreservation

Moraes

Matos

Graham

et al. 2015

Animal Reproduction Science

Self Cite

View full text Add to dashboard Cite

“…In these two studies, stallion spermatozoa from fresh ejaculates were treated with 6 µM IA for 5 min in Hepes-buffered Hank's balanced salt solution containing 1% BSA. Pretreatment of stallion spermatozoa with IA was also reported to be effective to some extent for IVF of in vitro-matured equine oocytes [3,4,8] and zona-free hamster oocytes [9][10][11][12]. In those studies, spermatozoa were pre-treated with IA in various combinations of concentrations (0.1 to 7.14 µM) and treatment p e r i o d s ( 1 t o 1 0 m i n ) , a n d t h e o p ti m a l concentrations and treatment periods of IA varied greatly among the reports.…”

Section: Discussionmentioning

confidence: 99%

“…Attempts to induce capacitation (and subsequent acrosome reaction) of stallion spermatozoa in vitro have been reported by treatments with the following substances; calcium ionophore A23187 (IA) [3][4][5][6][7][8][9], liposomes [10][11][12], and heparin [7,8,[13][14][15][16], and by incubation with oviductal epithelial cells [6]. Among them, the pretreatment of spermatozoa with IA before IVF appears to be most promising for successful IVF in equine species, since two foals have been born from IVF embryos which were derived from oocytes that had been matured in vivo and inseminated with spermatozoa treated with IA [5,17].…”

mentioning

confidence: 99%

Assay for Penetrability of Frozen-thawed Stallion Spermatozoa into Zona-free Hamster Oocytes.

2002

View full text Add to dashboard Cite

Abstract. In Japan, low availability of equine oocytes makes it very difficult to improve the in vitro fertilization (IVF) system of horses. In this situation, an assay for sperm penetrability into zona-free hamster oocytes could be useful as a simulator of the IVF system. The aim of this study was to clarify the optimum conditions of the pretreatment of frozen-thawed stallion spermatozoa with caffeine and ionophore A23187 (IA) in the penetration assay. Frozen-thawed stallion spermatozoa were washed and then pretreated with IA in modified BO solution (mBO) supplemented with caffeine. After the pretreatment, the spermatozoa were used for the penetration assay with zona-free hamster oocytes. At first, effect of increasing concentrations of caffeine (0-10 mM) were examined on the penetration of spermatozoa pretreated with 1 µM IA for 60 sec. The highest penetration rate after 10 h co-culture with oocytes was obtained for spermatozoa penetrated in the presence of 1 mM caffeine (49.0%). Next, when spermatozoa were pretreated with 0-1.0 µM IA for 0-180 sec in the presence of 1 mM caffeine, significantly higher penetration rates after 10 h co-culture with oocytes were obtained under the condition of 0.1 µM IA for 120 sec (52.2%) and 1.0 µM IA for 60 sec (40.9%). When oocytes were fixed 2, 4, 6, 8 and 10 h after IVF using spermatozoa treated with 0.1 µM IA for 120 sec in mBO containing 1 mM caffeine, the penetration rates increased significantly from 2 h (10.4%) to 4 h (21.7%) and from 6 h (27.5%) to 8 h (49.0%). These results indicate that pretreatment with 0.1 µM IA for 120 sec in the presence of 1 mM caffeine effectively promotes the penetration of frozen-thawed stallion spermatozoa into zona-free hamster oocytes, and that under this condition, spermatozoa might complete the acrosome reaction during 6 to 8 h of incubation after IA treatment.

show abstract

Comparison of the fertility of cryopreserved stallion spermatozoa with sperm motion analyses, flow cytometric evaluation, and zona-free hamster oocyte penetration

Cited by 67 publications

References 46 publications

Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing

Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing

Cholestanol-loaded-cyclodextrin improves the quality of stallion spermatozoa after cryopreservation

Assay for Penetrability of Frozen-thawed Stallion Spermatozoa into Zona-free Hamster Oocytes.

Contact Info

Product

Resources

About