Comparison of the Genetic Diversity of Wild and Captive Groups of Microcebus murinus Using the Random Amplified Polymorphic DNA Method

Neveu, H; Hafen, Thomas; Zimmermann, Elke; Rumpler, Y.

doi:10.1159/000052706

Cited by 28 publications

(18 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Average gene diversity across all loci (Nei, 1987) within Table 2. Analysis of molecular variance (AMOVA) for individuals from Emas National Park and Pantanal using Nei and Li (1979) (Cooper, 2000), carnivores (Ratnayeke et al, 2002), and primates (Neveu et al, 1998). Few studies using the RAPD technique have been reported on cervid species.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)

et al. 2007

View full text Add to dashboard Cite

The Pampas deer (Ozotoceros bezoarticus) is one of the most endangered Neotropical cervid with populations that have been drastically reduced to small and isolated ones, mainly because of its habitat destruction. Random amplified polymorphic DNA (RAPD) markers were used to analyze population divergence and genetic variation within and between two populations corresponding to distinct subspecies. The RAPD markers displayed substantial genetic variation with all animals possessing unique RAPD phenotypes over 105 polymorphic bands produced by 15 primers. An analysis of molecular variance (AMOVA) and a neighbor-joining cluster analysis were performed to assess levels of differentiation between populations. No differentiation was recorded and about 96.0% (P < 0.00001) of the total variance was attributable to variation within populations. This result is quite distinct from data obtained by the analysis of the mtDNA control region, and is discussed on the basis of genetic differences between the different markers and the male-biased dispersal patterns generally observed in the mammal species. The data presented herein are potentially useful for future taxonomic and genetic studies in this species, for the monitoring of the genetic variation observed within these populations, and for the development of management guidelines for its conservation.Keywords: Cervidae, Ozotoceros bezoarticus, RAPD, genetic diversity, population structure. Diversidade genética de duas populações brasileiras de Veado-campeiro (Ozotoceros bezoarticus, Linnaeus 1758)Resumo O Veado-campeiro (Ozotoceros bezoarticus) é uma das espécies de cervídeos neotropical mais ameaçadas devido à destruição de seu hábitat e conseqüente redução e isolamento de suas populações. Marcadores do tipo RAPD (Random amplified polymorphic DNA) foram utilizados na análise da divergência populacional e estimativa da variação genética dentro e entre duas populações correspondentes a diferentes subespécies. Os marcadores RAPD mostraram uma variação genética substancial, sendo que as 105 bandas polimórficas obtidas pelo uso de 15 primers produziram fenótipos únicos para todos os indivíduos analisados. Para avaliar o nível de diferenciação entre as populações, foi realizada uma análise da variância molecular (AMOVA) e uma análise de agrupamento utilizando o método de neighbor-joining. Nenhuma diferenciação foi observada, sendo aproximadamente 96,0% da variação encontrada atribuída à variação dentro das populações estudadas. Este resultado difere do obtido através da análise da região controle do mtDNA, e é discutido levando-se em consideração as diferenças genéticas entre os diferentes marcadores utilizados e o padrão de dispersão geralmente observado nas espécies de mamíferos (realizada principalmente pelos machos). Os dados aqui apresentados poderão ser úteis para futuros estudos taxonômicos e genéticos desta espécie, para o monitoramento da variação genética observada em suas populações e para o desenvolvimento de estratégias de manejo para sua conservação.Palavras...

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)

et al. 2007

View full text Add to dashboard Cite

show abstract

“…However, as noted above, drawbacks to this method (e.g., the extreme sensitivity of the procedure to PCR reaction conditions) have limited its application in studies of primate mating systems (Riedy et al, 1992), especially when compared with PCR-based microsatellite genotyping. Most recent applications of RAPD analysis in the primate literature involve estimating the degree of genetic variation within and between selected primate taxa (e.g., Lan et al, 1995;Neveu et al, 1998;Bachmann et al, 2000;Fausser et al, 2000;Ravaoarimanana et al, 2001) rather than as a tool for parentage analysis.…”

Section: Studies Of Mating Systems and Reproductive Strategiesmentioning

confidence: 99%

Molecular genetic approaches to the study of primate behavior, social organization, and reproduction

Fiore

2003

Am. J. Phys. Anthropol.

107

View full text Add to dashboard Cite

In the past several decades, the development of novel molecular techniques and the advent of noninvasive DNA sampling, coupled with the ease and speed with which molecular analyses can now be performed, have made it possible for primatologists to directly examine the fitness effects of individual behavior and to explore how variation in behavior and social systems influences primate population genetic structure. This review describes the theoretical connections between individual behavior and primate social systems on the one hand and population genetic structure on the other, discusses the kinds of molecular markers typically employed in genetic studies of primates, and summarizes what primatologists have learned from molecular studies over the past few decades about dispersal patterns, mating systems, reproductive strategies, and the influence of kinship on social behavior. Several important conclusions can be drawn from this overview. First, genetic data confirm that, in many species, male dominance rank and fitness are positively related, at least over the short term, though this relationship need not simply be a reflection of malemale contest competition over mates. More importantly, genetic research reveals the significance of female choice in determining male reproductive success, and documents the efficacy of alternative mating tactics among males. Second, genetic data suggest that the presumed importance of kinship in structuring primate social relationships needs to be evaluated further, at least for some taxa such as chimpanzees in which demographic factors may be more important than relatedness. I conclude this paper by offering several suggestions of additional ways in which molecular techniques might be employed in behavioral and ecological studies of primates (e.g., for conducting "molecular censuses" of unhabituated populations, for studying disease and host-parasite interactions, or for tracking seed fate in studies of seed dispersal) and by providing a brief introduction to the burgeoning field of nonhuman primate behavioral genetics. Yrbk Phys Anthropol 46:62-99, 2003.

show abstract

“…Methods for determining paternity [126] 14.4.2. Methods for assessing genetic variation; practical consequences [110,[127][128][129][130][131] 14.4.3. Genetic variation within existing populations of the species 14.4.4.…”

Section: Items For Husbandry Research 161mentioning

confidence: 99%

A ‘Checklist’ of Possible Items for Prosimian Husbandry Manuals and Research

Schulze¹,

Benirschke²,

Doyle³

et al. 1998

IJFP

View full text Add to dashboard Cite

Zoo populations of prosimians are usually so small that death or reproductive failure of a few individuals may lead to a considerable loss of genetic diversity. Losses due to inadequate captive conditions must be minimized. This paper provides ideas and literature references for practical improvements of captive conditions and could serve as a checklist for writers of husbandry manuals. Items for inclusion in husbandry manuals containing detailed information about prosimians are provided. The preservation of genetic diversity in captive populations and adaptations of wild populations to their habitats are discussed. A list of items proposed for husbandry research includes an identification key for subpopulations and subspecies, field data and a variety of other factors such as housing, climatic needs, behaviour, relation between animals and keepers, reproduction, health, animal welfare and stress. Care for individuals as well as the metapopulation is recommended. The list includes some items which are not generally regarded as husbandry matters, but which ought to be considered, especially when a species is held for conservation purposes within managed programmes which include both in situ and ex situ components. The paper was based on experience with Loris tardigradus, so the needs of some other species may be less well covered.

show abstract

Comparison of the Genetic Diversity of Wild and Captive Groups of Microcebus murinus Using the Random Amplified Polymorphic DNA Method

Cited by 28 publications

References 26 publications

Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)

Genetic diversity of two Brazilian populations of the Pampas deer (Ozotoceros bezoarticus, Linnaeus 1758)

Molecular genetic approaches to the study of primate behavior, social organization, and reproduction

A ‘Checklist’ of Possible Items for Prosimian Husbandry Manuals and Research

Contact Info

Product

Resources

About