Comparison of the genome size, endoreduplication, and ISSR marker polymorphism in eight Lotus (Fabaceae) species

Abstract

IntroductionThe genus Lotus (Fabaceae; previously Leguminosae) comprises nearly 200 annual and perennial species that are distributed worldwide, except in very cold regions and in some tropical areas of Southeast Asia and Central America (Allan et al., 2004;Escaray et al., 2012). Within this genus, more than 15% of the species are endangered, vulnerable, or rare (IUCN, 2015). Several species are important forage crops (e.g., L. corniculatus, L. uliginosus, L. tenuis, and L. subbiflorus) since their nutritional value is similar or even superior to that of white clover and alfalfa (Jones and Earle, 1966;Escaray et al., 2012).Lotus taxonomy is one of the most problematic within the tribe Loteae due to the limited number of appropriate discernable morphological traits, insufficient genetic description, and high morphological and biogeographical diversity (Grant and Small, 1996;Allan et al., 2004;Escaray et al., 2012). Therefore, in addition to morphological analyses, karyological and molecular studies should be applied for species identification and establishing taxonomic assignment and phylogenetic relationships.The majority of Lotus species have the basic chromosome number x = 6 or 7 and are mostly diploids. However, in some species both diploid and tetraploid accessions occur, while others include only tetraploid forms (e.g., L. corniculatus;Ferreira and Pedrosa-Harand, 2014). Genome sizes of the genus Lotus have been reported for 39 species only (20%), with a range from 0.56 to 2.80 pg/2C (

Genetic variations in Turkey cultivar and ecotype Medicago sativa species: cytological, total protein profile, and molecular characterization

“…Our findings partially confirm this phenomenon; Muş variety displayed the highest peak value in endoreduplication followed by Iside, Konya, and Plato. These findings are very similar with one of the first reports on identification and on endoreduplication of lotus species [34]. Brummer et al reported the chromosome numbers and nuclear DNA contents of 263 alfalfa and 20 Medicago falcata plant species using chromosome counts and flow cytometry.…”

Genome Size Diversity in Rare, Endangered, and Protected Orchids in Poland

“…This is of great importance for a such rare and valuable group of plants. Genome size estimation alone, or in combination with molecular markers were earlier used for Ocimum [ 42 ], Mentha [ 43 ], Lotus [ 44 ], Origanum [ 45 ], and Malva [ 46 ] species identification.…”

Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

“…Forty-one seedlings were used as controls. The leaf samples were prepared and analyzed as previously described [ 37 ], using propidium iodide (PI) for DNA staining. Petunia hybrida P×Pc6 (2.85 pg/2C; [ 38 ]) served as the internal standard.…”