SummaryAlthough studies in rodents and humans have evidenced a weaker effect of fat in comparison to carbohydrates on the suppression of food intake, very few studies have been carried out in this field in dogs. This study investigates the effects of a highcarbohydrate (HC) and a high-fat (HF) diets on subsequent food intake and blood satiety-related hormones in dogs. Diets differed mainly in their starch (442 vs. 271 g/ kg dry matter) and fat (99.3 vs. 214 g/kg dry matter) contents. Twelve Beagle dogs received the experimental diets at maintenance energy requirements in two experimental periods, following a cross-over arrangement. In week 7 of each period, blood concentrations of active ghrelin, glucagon-like peptide (GLP-1), peptide YY, insulin, and glucose were determined before and at 30, 60, 120, 180, and 360 min postfeeding. The following week, intake of a challenge food offered 180 min after the HC and HF diets was recorded over two days. In comparison to the dogs on the HC diet, those on the HF diet had a higher basal concentration of GLP-1 (p = .010) and a higher total area under the curve over 180 min post-prandial (tAUC 0-180 ) (p = .031). Dogs on the HC diet showed a higher elevation of ghrelin at 180 min (p = .033) and of insulin at 360 min (p = .041), although ghrelin and insulin tAUC 0-180 did not differ between the two diets (p ˃ .10). Diet had no effect on challenge food intake (p ˃ .10), which correlated with the tAUC 0-180 of ghrelin (r = .514, p = .010), insulin (r = −.595, p = .002), and glucose (r = −.516, p = .010). Feeding a diet high in carbohydrate or fat at these inclusion levels does not affect the feeding response at 180 min post-prandial, suggesting a similar short-term satiating capacity.
K E Y W O R D SBeagle, dietary fat, gut hormones, short-term satiety, starch
| INTRODUCTIONThe effect of the main macronutrients on food intake and on satietyrelated gut hormones has been widely studied in humans and rats as the incidence of obesity and metabolic-related diseases gradually increases in Western countries. However, there have been comparatively few studies carried out in dogs and these have mainly focused on the effect of protein and fibre (Bosch et al., 2009;Weber et al., 2007). According to studies in humans, consumption of fat is closely related to body fat mass beyond any other individual factor (Tucker & Kano, 1992), and an increase in the fat to carbohydrate content of diets has been related to an increased energy intake in humans (Blundell, Burley, Cotton, & Lawton, 1993) and rats (Warwick, 2003) during ad libitum feeding tests. Over-consumption of fat-rich diets in these species may be associated with their high palatability, which can reinforce feeding behaviour even when energy needs have been satisfied Erlanson-Albertsson, 2005), but also to a weaker satiating capacity of fat. In this respect, a series of studies in humans (Blundell et al., 1993) and rats (Gaysinskaya, Karatayev, Chang, & Leibowitz, 2007) have evidenced a higher capacity of carbohydrates vs. fat to supp...