Comparison of the Immulite and RIA assay methods for measuring peripheral blood progesterone levels in Greyhound bitches

Chapwanya, Aspinas; Clegg, Tracy A.; Stanley, Paul; Vaughan, Laurence M.

doi:10.1016/j.theriogenology.2008.05.047

Cited by 34 publications

(50 citation statements)

References 5 publications

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“…The serum P 4 concentration was 3.7 ± 0.9 ng/ml on the LH surge day and 31.3 ± 10.2 ng/ml on the day of TCI (Table 1). Ovulation was assumed to have occurred when serum P 4 concentration was >5 ng/ml, and the AI day was confirmed [1,2,4,14]. Serum P 4 concentrations on the day of TCI in this study were >15 ng/ml.…”

Section: Resultsmentioning

confidence: 78%

“…The P 4 assay method we used has been previously used to estimate the LH surge day [1,2,4,7,8,14]. Since the measurement methods for chemiluminescence immunoassay, radioimmunoassay and enzyme immunoassay were almost perfectly correlated, the reference value of the P 4 assay method in this study was fixed [2,4,14].…”

Section: Discussionmentioning

confidence: 99%

“…Since the measurement methods for chemiluminescence immunoassay, radioimmunoassay and enzyme immunoassay were almost perfectly correlated, the reference value of the P 4 assay method in this study was fixed [2,4,14]. Subsequently, the LH surge and TCI days were also estimated by measuring serum P 4 concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…The timing of insemination in relation to the luteinizing hormone (LH) surge was determined by monitoring serum progesterone (P 4 ) concentration using an automated chemiluminescence immuno analyzer (Spotchem Vidas SV-5020, Arkray, Kyoto, Japan) [1,2,4,7,8,14]. The start date of proestrus was confirmed by daily observation of vulvar swelling and estrous bleeding.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Hayashi

Morita

Aso

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. We evaluated transcervical insemination (TCI) using frozen semen by flexible endoscopy in dogs. Eight female and eight male beagles were used in this study. A flexible endoscope and a washing tube were used for TCI. A tracheal tube was used as an alternative to the penis and was an auxiliary for inserting the flexible endoscope. The mean time required to insert the washing tube into the external os of the uterus after inserting the endoscope into the tracheal tube was 7.5 min. Slight or mild pain was observed in all bitches during TCI. However, TCI could be easily performed with retention in all bitches and without sedation anesthesia. The tracheal tube was useful to ensure the visual field using air sufflation. Clinical signs suspicious of infection were not observed in any bitches from the TCI to the pregnancy diagnosis. The conception rate was 87.5%, and the mean number of fetuses was 6.3. TCI using a flexible endoscope in bitches was performed quickly with minimal invasiveness. We present a new method of TCI in dogs. This method should be studied in small and large breeds to obtain more detailed results. Artificial insemination (AI) using frozen semen in dogs has been increasing in recent years. However, AI using cryopreserved semen generally yields lower pregnancy rates with vaginal deposition than with uterine deposition because of the short life span of frozen-thawed spermatozoa [5,19]. Therefore, AI methods usually use uterine deposition with frozen semen. Surgical insemination is highly invasive and requires general anesthesia, whereas transcervical insemination (TCI) using metal catheterization is minimally invasive but requires a skilled technique [24]. In contrast, TCI using a rigid endoscope is easy and less invasive [13,30]. TCI with a flexible endoscope is also less invasive because the scope is soft, and it has an air supply and instrument channel, which endows it with good operability [21,29]. Therefore, a flexible endoscope might be useful as a new tool for TCI.The purpose of this study was to evaluate TCI using frozen semen by flexible endoscope in dogs. MATERIALS AND METHODSEight female beagles aged 1-8 years (mean ± standard deviation: SD, 4.1 ± 2.5 years) and weighing 10.7-15.1 kg (mean ± SD, 12.8 ± 1.3 kg) were used in this study; 4 were parous, and the others were nulliparous. Eight male beagles aged 1-7 years (mean ± SD, 3.6 ± 1.8 years) and weighing 11.4-15.8 kg (mean ± SD, 13.1 ± 1.4 kg) were used as sperm donors. Unrelated dogs were used to prevent inbreeding. This study was performed in accordance with the Guide for the Experimentation of Animals of the College of Bioresource Sciences, Nihon University (AP11B018, 2010).The sperm-rich fraction of a single ejaculate was collected from each dog by digital manipulation [11]. The collected semen was centrifuged at 400 × g for 5 min. The supernatant was discarded, and the pellets were gently suspended in 1 ml with egg-yolk Tris-fructose citrate extender supplemented with 1% Orvus ES paste (OEP) [26][27][28]. Sperm concentration w...

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Section: Resultsmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Hayashi

Morita

Aso

et al. 2013

The Journal of Veterinary Medical Science

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“…Besides biological factors and sample management, the assay method, the storage conditions and handling also influence the final concentrations measured (Molnár et al, 1984;Navarro et al, 1990;Hock et al, 1999;Thuróczy et al, 2003;Volkmann, 2006;Chapwanya et al, 2008;Tahir et al, 2013;Schumacher et al, 2014).…”

mentioning

confidence: 99%

Effect of ELISA kit manufacturing process and incubation time on progesterone concentration measured in dog serum for ovulation diagnosis – Short communication

Thuróczy

Reiczigel

2016

Acta Veterinaria Hungarica

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Twenty-two serum samples of healthy bitches were tested with the frozen and lyophilised version of the same ELISA kit (Quanticheck, Faculty of Veterinary Science, Budapest, Hungary). Samples were chosen on the basis of their progesterone (P 4 ) concentrations, which were between 1.00 and 20.00 ng/mL. As it is well known, this range has the highest clinical relevance in ovulation diagnosis. Both types of microplates were read at 15-min intervals from the 15th until the 90th minute (min) of incubation, and the results were compared with those of frozen plates at 60 min of incubation as 100 percent. Lyophilised microplates gave on average 18 percent higher results than the frozen version at equal incubation times. The highest difference between lyophilised and frozen samples was observed at 45 and 60 min of incubation. Ninety-four percent of the reaction in the frozen microplate occurred in the first 15 min, and during the subsequent 30 min the reaction seemingly stopped. After the 45th min of incubation, this 94 percent increased to 108 percent in the subsequent 30 min, which remained the final approximate result at the end of the 90 min of incubation. In contrast to the frozen microplate, the measured concentration increased continuously in the lyophilised version and reached the highest level at the 60th min. The results of the lyophilised microplate reached the same level at 30 min of incubation as those of the frozen version at 60 min. In conclusion, a mechanical increase or decrease of the incubation time does not generate a linear change in the test results. This study demonstrated that the results of a series of samples collected from the same bitch cannot be compared if they are measured with different laboratory methods or different ELISA kits.

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Blood periovulatory progesterone quantification using different techniques in the dog

Gloria

Contri

Carluccio

et al. 2018

Animal Reproduction Science

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Comparison of the Immulite and RIA assay methods for measuring peripheral blood progesterone levels in Greyhound bitches

Cited by 34 publications

References 5 publications

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Evaluation of Transcervical Insemination using Frozen Semen by Flexible Endoscope in Dogs

Effect of ELISA kit manufacturing process and incubation time on progesterone concentration measured in dog serum for ovulation diagnosis – Short communication

Blood periovulatory progesterone quantification using different techniques in the dog

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