Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus

Kwon, Se Ryun

doi:10.7847/jfp.2012.25.3.159

Cited by 6 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More than 80 serotypes of V. parahaemolyticus have been described worldwide, based on the O and K antigenic properties [22]. To date, some BGs have been produced from other Vibrio species ( V. cholera , V. anguillarum , V. vulnificus ) by using the lysis E -gene system [4,5,23,24], but no BGs have been reported from V. parahaemolyticus . In the present study, we determined the MIC of various chemicals (six acids and one alkali) on V. parahaemolyticus cells and characterized their efficiencies to produce V. parahaemolyticus ghosts (VPGs).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

Park

Vinod

et al. 2016

IJMS

View full text Add to dashboard Cite

Acellular bacterial ghosts (BGs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene E of bacteriophage PhiX174. In this study, Vibrio parahaemolyticus ghosts (VPGs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. The MIC values of the respective chemicals were 3.125, 6.25, <50.0, 25.0, 6.25, 1.56, and 0.781 mg/mL. Except for boric acid, the lysis efficiency reached more than 99.99% at 5 min after treatment of all chemicals. Among those chemicals, NaOH-induced VPGs appeared completely DNA-free, which was confirmed by quantitative real-time PCR. Besides, lipopolysaccharides (LPS) extracted from the NaOH-induced VPGs showed no distinctive band on SDS-PAGE gel after silver staining. On the other hand, LPS extracted from wild-type bacterial cells, as well as the organic acids-induced VPGs showed triple major bands and LPS extracted from the inorganic acids-induced VPGs showed double bands. It suggests that some surface structures in LPS of the NaOH-induced VPGs may be lost, weakened, or modified by the MIC of NaOH. Nevertheless, Limulus amoebocyte lysate assay revealed that there is no significant difference in endotoxic activity between the NaOH-induced VPGs and wild-type bacterial cells. Macrophages exposed to the NaOH-induced VPGs at 0.5 × 106 CFU/mL showed cell viability of 97.9%, however, the MIC of NaOH did not reduce the cytotoxic effect of wild-type bacterial cells. Like Escherichia coli LPS, the NaOH-induced VPGs are an excellent activator of pro-inflammatory cytokines (IL-1β and iNOS), anti-inflammatory cytokine (IL-10), and dual activities (IL-6) in the stimulated macrophage cells. On the other hand, the induction of TNF-α mRNA was remarkable in the macrophages exposed with wild-type cells. Scanning electron microscopy showed the formation of trans-membrane lysis tunnel structures in the NaOH-induced VPGs. SDS-PAGE and agarose gel electrophoresis also confirmed that cytoplasmic proteins and genomic DNA released from the VPGs to culture medium through the lysis tunnel structures. Taken together, all these data indicate that the NaOH-induced VPGs show the potency of a safe, economical, and effective inactivated bacterial vaccine candidate.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

Park

Vinod

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…Vaccination concurrently triggered both innate immune response and adaptive immunity, which is very important for the control of bacterial diseases. Traditional inactivation of bacteria by formalin may destroy bacterial antigenic epitopes (Kwon, 2012). The study found that bacterial ghost can effectively stimulate innate and adaptive immune responses (Felnerova et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The Gram-negative bacteria, under the impact of Lysis E, result in the expulsion of cytoplasmic contents and the formation of the bacterial shell structure, namely bacterial ghost vaccine. In recent years, a number of ghost vaccines have been studied, such as Escherichia coli (Mayr et al, 2005), E. tarda (Lee et al, 2008), V. anguillarum (Kwon et al, 2009), Aeromonas hydrophila (Chu et al, 2008) Flavobacterium cloumnare (Zhu et al, 2012) and V. vulnificus (Kwon, 2012). With bacterial ghosts, surface protein antigens remain intact, and these induce the body's humoral and cellular immune response (Felnerova et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Immune responses to Vibrio vulnificus formalin‐killed vaccine and ghost vaccine in Scophthalmus maximus

Zhou

Liu

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

In this research, Vibrio vulnificus formalin‐killed (FKCs) vaccine and ghost (VVGs) vaccine were successfully developed, and shown to prevent vibriosis of Scophthalmus maximus resulting from V. vulnificus. The antibody titre of FKCs and VVGs vaccine was 1: 28 and 1: 211. The RPS of FKCs and VVGs vaccine was 60% and 80%. In order to improve the understanding of vaccine protection mechanism, transcriptome data was used to analyse the immune response of S. maximus infected with V. vulnificus after vaccination with FKCs and VVGs vaccine. In the SmCon and SmIV groups, a series of innate immune‐related genes were upregulated (such as, TLR5, Tp12, AP‐1 and IL‐1β) or downregulated (such as, CASP6 and CASP8), which suggested that the immune protection mechanism induced by inactivated vaccine was similar to that of autoimmune response. In the SmIV and SmGho group, a number of innate and adaptive immune‐related genes (such as, STAT1, IFN‐γ and MHC Ia) were activated, in which the expression of these genes was higher in SmGho, and VVGs vaccine induced stronger innate and acquired immune responses. In conclusion, the results lay a foundation for further study on the molecular mechanisms of immune protection induced by VVGs vaccine and FKCs vaccine.

show abstract

Research progress on bacterial ghosts as novel fishery vaccines

Wang

Wei

et al. 2022

Aquaculture

View full text Add to dashboard Cite

Comparison of the immunogenicity between bacterial ghost and formalin-killed bacteria for Vibrio vulnificus

Cited by 6 publications

References 24 publications

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

Characterization of Chemically-Induced Bacterial Ghosts (BGs) Using Sodium Hydroxide-Induced Vibrio parahaemolyticus Ghosts (VPGs)

Immune responses to Vibrio vulnificus formalin‐killed vaccine and ghost vaccine in Scophthalmus maximus

Research progress on bacterial ghosts as novel fishery vaccines

Contact Info

Product

Resources

About