Comparison of the immunolabeling of heated and deplasticized epoxy sections on the electron microscopical level

Brorson, Sverre‐Henning

doi:10.1016/s0968-4328(99)00030-x

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…Hydrogen peroxide is commonly employed in immunological electron microscopy to partially dissolve the resin surface of epoxy resin‐embedded tissue sections during immunolabeling techniques (Brorson,2001). The etching effect of these chemicals depends on partial resinous matrix dissolution, breaking epoxy resin bonds through substrate oxidation (Baskin et al,1979; Brorson,1999). The etching of both fiber posts with H 2 O 2 10% (Figs.…”

Section: Discussionmentioning

confidence: 99%

Surface treatment of glass fiber and carbon fiber posts: SEM characterization

Naves¹,

Santana²,

Castro³

et al. 2011

Microscopy Res & Technique

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Morphology, etching patterns, surface modification, and characterization of 2 different fiber posts: Gfp, Glass fiber post; and Cfp, carbon fiber were investigated by SEM analysis, after different surface treatments. Thirty fiber posts, being 15 Gfp and 15 Cfp were divided into a 5 surface treatments (n = 3): C-alcohol 70% (control); HF 4%-immersion in 4% hydrofluoric acid for 1min; H(3) PO(4) 37%-immersion in 37% phosphoric acid for 30s; H(2) O(2) 10%-immersion in 10% hydrogen peroxide for 20 min; H(2) O(2) 24%-immersion in 24% hydrogen peroxide for 10 min. Morphology, etching patterns, surface modification and surface characterization were acessed by SEM analysis. SEM evaluation revealed that the post surface morphology was modified following all treatment when compared with a control group, for both type of reinforced posts. HF seems to penetrate around the fibers of Gfp and promoted surface alterations. The Cfp surface seems to be inert to treatment with HF 4%. Dissolution of epoxy resin and exposure of the superficial fiber was observed in both post groups, regardless the type of reinforcing fiber, H(2) O(2) in both concentrations. Relative smooth surface area was produced by H(3) PO(4) 37% treatment, but with similar features to untreated group. Surface treatment of fiber post is a determinant factor on micromechanical entanglement to resin composite core. Post treatment with hydrogen peroxide resulted strength of carbon and glass/epoxy resin fiber posts to resin composite core.

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Section: Discussionmentioning

confidence: 99%

Surface treatment of glass fiber and carbon fiber posts: SEM characterization

Naves¹,

Santana²,

Castro³

et al. 2011

Microscopy Res & Technique

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“…Immunogold labelling for TEM analyses was conducted on ultrathin sections (90 nm) obtained with a Leica UCT ultramicrotome (Leica Microsystems). Procedures were performed on nickel grid-mounted sections floating in drops [28]. Epoxy ultrathin sections were treated with 10% hydrogen peroxide (H 2 O 2 ) for 10 min, and then they were pre-incubated in 10 mM PBS for 10 min.…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

Cyst Nematode Infection Elicits Alteration in the Level of Reactive Nitrogen Species, Protein S-Nitrosylation and Nitration, and Nitrosoglutathione Reductase in Arabidopsis thaliana Roots

et al. 2020

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Reactive nitrogen species (RNS) are redox molecules important for plant defense against pathogens. The aim of the study was to determine whether the infection by the beet cyst nematode Heterodera schachtii disrupts RNS balance in Arabidopsis thaliana roots. For this purpose, measurements of nitric oxide (NO), peroxynitrite (ONOO−), protein S-nitrosylation and nitration, and nitrosoglutathione reductase (GSNOR) in A. thaliana roots from 1 day to 15 days post-inoculation (dpi) were performed. The cyst nematode infection caused generation of NO and ONOO− in the infected roots. These changes were accompanied by an expansion of S-nitrosylated and nitrated proteins. The enzyme activity of GSNOR was decreased at 3 and 15 dpi and increased at 7 dpi in infected roots, whereas the GSNOR1 transcript level was enhanced over the entire examination period. The protein content of GSNOR was increased in infected roots at 3 dpi and 7 dpi, but at 15 dpi, did not differ between uninfected and infected roots. The protein of GSNOR was detected in plastids, mitochondria, cytoplasm, as well as endoplasmic reticulum and cytoplasmic membranes. We postulate that RNS metabolism plays an important role in plant defense against the beet cyst nematode and helps the fine-tuning of the infected plants to stress sparked by phytoparasitic nematodes.

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“…O teste de recuperação de antígenos por aquecimento foi realizado utilizando-se dois diferentes tampões (tampão citrato 10 mM pH 6,0 ou EDTA 1 mM pH 8,0) e três diferentes temperaturas (55ºC, 65ºC ou 95ºC), como descrito por Brorson (1999). As telas contendo os cortes receberam o seguinte tratamento, antes de serem submetidas a imunomarcação:…”

Section: Preparação De Anticorpos Policlonais Contra Nrunclassified

Caracterização bioquímica e regulação da nitrato redutase na macroalga marinha <i>Gracilaria tenuistipitata</i> (Rhodophyta)

Lopes¹

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Comparison of the immunolabeling of heated and deplasticized epoxy sections on the electron microscopical level

Cited by 7 publications

References 17 publications

Surface treatment of glass fiber and carbon fiber posts: SEM characterization

Surface treatment of glass fiber and carbon fiber posts: SEM characterization

Cyst Nematode Infection Elicits Alteration in the Level of Reactive Nitrogen Species, Protein S-Nitrosylation and Nitration, and Nitrosoglutathione Reductase in Arabidopsis thaliana Roots

Caracterização bioquímica e regulação da nitrato redutase na macroalga marinha <i>Gracilaria tenuistipitata</i> (Rhodophyta)

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