Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides

Olano-Martín, Estíbaliz; Gibson, Glenn R.; Rastall, Robert A.

doi:10.1046/j.1365-2672.2002.01719.x

Cited by 252 publications

(166 citation statements)

References 34 publications

(68 reference statements)

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“…It is thought that this is due to the reduction of pathogenic bacteria such as Shigella, Salmonella, Klebsiella, Enterobacter, Proteus and Citrobacter from intestine. This is supported by Olano-Martin et al (2002) who observed that pectin stimulated the growth of certain strains of Bifidobacteria and Lactobacillus in vitro. These bacteria are considered to be directly related to the health of the large intestine and their concentrations depict a healthy microflora population.…”

Section: Pectin and Detoxificationmentioning

confidence: 54%

Plant Pectin: A Potential Source for Cancer Suppression

Niture¹

2013

American Journal of Pharmacology and Toxicology

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Pectin is a branched polysaccharide present widely in a variety of fruits. It has been of high interest to cancer biologists due to its involvement in metal/carcinogen detoxification and its anti-carcinogenic properties. In particular, Citrus Pectin (CP) and Modified Citrus Pectin (MCP) have been shown to have a significant inhibitory role in cancer cell metastasis, invasion, angiogenesis and survival. The interactions and inactivation of oncogenes by CP and MCP in prostate, breast, liver, lung, melanoma and multiple myeloma cancers suggest that CP and MCP could play an important role in cancer chemotherapy and chemoprevention. In this review, we focus on the biological function of pectin with respect to its properties, sources, structure and its potential role in carcinogen detoxification and cancer suppression.

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Section: Pectin and Detoxificationmentioning

confidence: 54%

Plant Pectin: A Potential Source for Cancer Suppression

Niture¹

2013

American Journal of Pharmacology and Toxicology

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“…This is due to the high degree of methoxylation of the pectin used in this study (Sigma-Aldrich, 2006). Methoxylation decreases the growth rate of pectin-fermenting bacteria (Olano-Martin et al, 2002). The methyl group can be esterified with a release of methanol (Drochner et al, 2004) but, unlike glucuronic acid, the methyl group is unusable by pectinolytic bacteria (Hall and Herejk, 2001) for ATP generation and growth.…”

Section: Figurementioning

confidence: 99%

The source of fermentable carbohydrates influences the in vitro protein synthesis by colonic bacteria isolated from pigs

Bindelle

Buldgen

Wavreille

et al. 2007

Animal

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Two in vitro experiments were carried out to quantify the incorporation of nitrogen (N) by pig colonic bacteria during the fermentation of dietary fibre, including non-starch polysaccharides and resistant starch. In the first experiment, five purified carbohydrates were used: starch (S), cellulose (C), inulin (I), pectin (P) and xylan (X). In the second experiment, three pepsin-pancreatin hydrolysed ingredients were investigated: potato, sugar-beet pulp and wheat bran. The substrates were incubated in an inoculum, prepared from fresh faeces of sows and a buffer solution providing 15 N-labelled NH 4 Cl. Gas production was monitored. Bacterial N incorporation (BNI) was estimated by measuring the incorporation of 15 N in the solid residue at halftime to asymptotic gas production (T/2). The remaining substrate was analysed for sugar content. Short-chain fatty acids (SCFA) were determined in the liquid phase. In the first experiment, the fermentation kinetics differed between the substrates. P, S and I showed higher rates of degradation (P , 0.001), while X and C showed a longer lag time and T/2. The sugar disappearance reached 0.91, 0.90, 0.81, 0.56 and 0.46, respectively, for P, I, S, C and X. Among them, S and I fixed more N per gram substrate (P , 0.05) than C, X and P (22.9 and 23.2 mg fixed N per gram fermented substrate v. 11.3, 12.3 and 9.8, respectively). Production of SCFA was the highest for the substrates with low N fixation: 562 and 565 mg/g fermented substrate for X and C v. 290 to 451 for P, I and S (P , 0.01). In the second experiment, potato and sugar-beet pulp fermented more rapidly than wheat bran (P , 0.001). Substrate disappearance at T/2 varied from 0.17 to 0.50. BNI were 18.3, 17.0 and 10.2 fixed N per gram fermented substrate, for sugar-beet pulp, potato and wheat bran, respectively, but were not statistically different. SCFA productions were the highest with wheat bran (913 mg/g fermented substrate) followed by sugar-beet pulp (641) and potato (556) (P , 0.05). The differences in N uptake by intestinal bacteria are linked to the partitioning of the substrate energy content between bacterial growth and SCFA production. This partitioning varies according to the rate of fermentation and the chemical composition of the substrate, as shown by the regression equation linking BNI to T/2 and SCFA (r 2 5 0.91, P , 0.01) and the correlation between BNI and insoluble dietary fibre (r 5 20.77, P , 0.05) when pectin was discarded from the database.

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“…Also, the cost of producing prebiotic materials such as XOS becomes 45% lower than that of other conventional fruit pulp industries as shown in the earlier research (Olano-Martin et al, 2002). Hence, the current research prefers XOS production from orange fruit wastes.…”

Section: Introductionmentioning

confidence: 81%

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

Gupta¹,

Agrawal²,

Hegde³

2017

Afr. J. Biotechnol.

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Xylooligosaccharides (XOS) are sugar oligomers made up of xylose units with the chain length of 2 to 10 and are considered non-digestible food ingredients. It is mainly produced from xylan hydrolysis. XOS have a characteristic of prebiotic by promoting the growth of probiotic organisms. They have various physiological effects such as reducing cholesterol, maintaining the gastrointestinal health and improving the biological availability of calcium, animal feed, anti-cancerous. The production of XOS from agricultural residues offers great scope to the nutraceutical and pharmaceutical industries as the raw material is cheap and abundantly available. The driving force of this study was to produce XOS from the organic wastes such as orange fruit wastes. These wastes are rich in xylan which can be used as a renewable material for producing XOS. XOS can be obtained by chemical or enzymatic method, but due to the yield of toxic by-product, enzymatic production is preferred. In the enzymatic extraction method, acetic acid was used to prepare pellets from dried orange peels powder followed by xylanase enzyme degradation performed at 2, 4, 6 and 8 h. Samples containing XOS were chromatographed on HPLC system having a fluorescence detector (Ex320 nm, Em420 nm). The column used was Agilent C18 of length 250 mm and 4.6 mm internal diameter.

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Comparison of the in vitro bifidogenic properties of pectins and pectic-oligosaccharides

Cited by 252 publications

References 34 publications

Plant Pectin: A Potential Source for Cancer Suppression

Plant Pectin: A Potential Source for Cancer Suppression

The source of fermentable carbohydrates influences the in vitro protein synthesis by colonic bacteria isolated from pigs

Value addition of orange fruit wastes in the enzymatic production of xylooligosaccharides

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