Comparison of the Indirect Fluorescent Antibody Test and Methylene Blue Dye Test for Detection of Antibodies to Toxoplasma Gondii

Walton, Bryce C.; Benchoff, Barbara M.; Brooks, Wiley H.

doi:10.4269/ajtmh.1966.15.149

Cited by 83 publications

(28 citation statements)

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“…Certain serologic methods are of little help in diagnosing congenital toxoplasmosis. This is especially true for some CF or IHA tests [606]. Results with these tests may be weakly positive or even negative in a newborn with congenital toxoplasmosis, and in the infant's mother.…”

Section: Demonstration Of Antibodies In Serum and Body Fluidsmentioning

confidence: 95%

“…A positive reaction is detected by the bright yellow-green fluorescence of the organisms seen on examination by fluorescence microscopy. In general, qualitative agreement with the dye test and IFA test has been excellent [606]. Despite the claims of many workers, reliable and reproducible quantitative titers frequently are difficult to obtain.…”

Section: Sabin-feldman Dyementioning

confidence: 98%

“…The most common serologic tests in use at present for diagnosis of T. gondii infection and toxoplasmosis are the Sabin-Feldman dye test [10], the indirect hemagglutination (IHA) test [127], the IFA test [606], the agglutination test [607], the ELISA [608][609][610][611], and the immunosorbent agglutination assay (ISAGA) [612][613][614]. Certain serologic methods are of little help in diagnosing congenital toxoplasmosis.…”

Section: Demonstration Of Antibodies In Serum and Body Fluidsmentioning

confidence: 99%

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Toxoplasmosis

Remington¹,

McLeod²,

Wilson³

et al. 2011

Infectious Diseases of the Fetus and Newborn

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Section: Demonstration Of Antibodies In Serum and Body Fluidsmentioning

confidence: 95%

Section: Sabin-feldman Dyementioning

confidence: 98%

Section: Demonstration Of Antibodies In Serum and Body Fluidsmentioning

confidence: 99%

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Toxoplasmosis

Remington¹,

McLeod²,

Wilson³

et al. 2011

Infectious Diseases of the Fetus and Newborn

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“…The serum samples were supplied by members of the NRCT network, and the presence of IgG or IgM antibodies was confirmed based on the results of the reference technique using at least two different methods for each antibody isotype, namely, the indirect immunofluorescence assay (IFAT-G) (19) and dye test (20)(21)(22) for IgG detection, and IFAT-M and immunosorbent agglutination assay (ISAGA-M; bioMérieux SA, Marcy-l'Etoile, France) (23) for IgM detection. IgA levels were not assessed.…”

Section: Samplesmentioning

confidence: 99%

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

et al. 2016

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h Toxoplasmosis, a benign infection, is asymptomatic or paucisymptomatic in over 80% of cases, except in immunocompetent patients suffering from ocular toxoplasmosis or in immunocompromised patients with opportunistic or congenital toxoplasmosis. Diagnosis is based mainly on serology testing. Thus, we compared the performance of the nine most commonly used commercial automated or semiautomated immunoassays for IgG and IgM Toxoplasma gondii antibody detection, that is, the Advia Centaur, Architect, AxSYM, Elecsys, Enzygnost, Liaison, Platelia, VIDAS, and VIDIA assays. The assays were conducted on four panels of serum samples derived during routine testing from patients with an interfering disease and who exhibited a low IgG antibody level in one of two clinical settings, namely, acute or chronic toxoplasmosis. As a result, IgG sensitivities ranged from 97.1% to 100%, and IgG specificities ranged from 99.5% to 100%. For IgG quantification, strong differences in IgG titers (expressed in IU/ml) were noted depending on the assay used. IgM sensitivities ranged from 65% to 97.9%, and IgM specificities ranged from 92.6% to 100%. For defining the best serological strategies to be implemented, it appears crucial to compare the diagnostic performance of the different tests with respect to their specificity and sensitivity in detecting the presence of IgG and IgM antibodies.

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“…If the clinical situation suggests toxoplasmosis, a fourfold rise in antibody titers, or even stable SabinFeldman or IgG-IFA titers of 1: 1,000 or greater or IgM-IFA titers of 1: 10 or greater, is compatible with the diagnosis. Problems in interpretation arise from the high prevalence of anti-Toxoplasma antibodies in the general population {22) or from missing the diagnostic fourfold rise in titers by the time the first sample is drawn [60]. The latter consideration has led investigators to suggest obtaining baseline serological studies in patients at risk [21}.…”

Section: Focal Brain Abnormalitiesmentioning

confidence: 99%

Neurological complications of acquired immune deficiency syndrome: Analysis of 50 patients

Snider

Simpson

Nielsen

et al. 1983

Annals of Neurology

1,298

331

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Fifty patients with acquired immune deficiency syndrome had complications affecting the central or peripheral nervous systems or both. The patients were either male homosexuals, intravenous drug abusers, or recently arrived Haitian refugees. They ranged in age from 25 to 56. Central nervous system complications were of four kinds: (1) Infections included Toxoplasma gondii abscesses in 5 patients, progressive multifocal leukoencephalopathy in 2, cryptococcal meningitis in 2, Candida albicans in 1, and possible Mycobacterium avium intracellulare in 3. Eighteen patients suffered a subacute encephalitis possibly attributable to cytomegalovirus infection. (2) Tumors consisted of primary lymphoma of the brain in 3 patients and meningeal invasion by systemic lymphoma in 4. (3) Vascular complications included nonbacterial thrombotic endocarditis in 2 patients and cerebral hemorrhages in the setting of thrombocytopenia in 3. (4) Undiagnosed central nervous system problems were evidenced as focal brain lesions in 3 patients and self-limiting aseptic meningitis in 4. Peripheral neuropathy occurred in 8 patients.

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Comparison of the Indirect Fluorescent Antibody Test and Methylene Blue Dye Test for Detection of Antibodies to Toxoplasma Gondii

Cited by 83 publications

References 0 publications

Toxoplasmosis

Toxoplasmosis

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

Neurological complications of acquired immune deficiency syndrome: Analysis of 50 patients

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