Comparison of the indirect fluorescent antibody test with agglutination, complement-fixation, and Coombs tests for<i>Brucella</i>antibody

Edwards, J M; Tannahill, A. J.; Bradstreet, C. M. Patricia

doi:10.1136/jcp.23.2.161

Cited by 24 publications

(11 citation statements)

References 3 publications

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“…None of these, however, is sufficiently specific (Wilkinson, 1966;Kerr et al 1968;Diaz Maravi-Poma & Rivero, 1976;White, 1978). Direct determination of brucella-specific IgM, IgG and IgA was made possible by the use of primary assays such as IFA (Edwards, Tannahill & Bradstreet, 1970), RIA (Parratt et al 1977) and more recently by ELISA (Magee 1980;Gilbert & Hawes, 1981;De Klerk & Anderson 1985;Strannegard, Araj & Fattah, 1985;Araj et al 1986). These tests avoid the complications of non-agglutinable antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…These tests, however, suffer from a high false negative result rate, take 1-2 days to perform, usually require rising titres for diagnosis and cannot per se differentiate the classes of antibodies involved (Kerr et al 1968;Buchanan & Faber, 1980;Klein & Behan, 1981). Tests such as the Coombs anti-human-globulin (Otero, Palenque & Noriega, 1982) complement fixation (Macdonald & Elmslie, 1967) indirect fluorescent antibody (IFA) (Biegeleisen, Bradshaw & Moody, 1962;Edwards, Tannahill & Bradstreet, 1970) and radioimmunoassay (RIA) (Parratt et al 1977) have been used to differentiate immunoglobulin (Ig) classes in patients with brucellosis.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings

Araj

Lulu

Mustafa

et al. 1986

J. Hyg.

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SUMMARYAn enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of brucella-specific IgG, IgM and Brucella ELISA immunoglobulins (Ig) were detected in some individuals in the control groups but the majority of these had titres of < 100 for IgG, IgM, and IgA. However, patients with brucellosis had significantly higher ELISA titres in all classes of Ig than controls but the sensitivity and specificity within each Ig class varied with the titre considered. At a titre of > 1600 the brucella IgG had a sensitivity and specificity of 98 % for patients with acute or chronic brucellosis; this decreased with lower reciprocal titres. The brucella IgM titre of > 400 had a sensitivity of 98 % and a specificity of 98 % for patients with acute brucellosis.However, in patients with chronic brucellosis the brucella IgM was very low. The brucella IgA titre of > 200 showed a sensitivity of 98 % and a specificity of 99 % for patients with either acute or chronic brucellosis. This study indicates that brucella ELISA is a rapid, sensitive and specific assay, provides a profile of Ig classes in the diagnosis of acute and chronic brucellosis, is useful for mass screening and could be considered the method of choice for the serological diagnosis of brucellosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings

Araj

Lulu

Mustafa

et al. 1986

J. Hyg.

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“…Indirect primary binding assays usually rely on antibody present in test serum (or other body fluids) reacting with immobilized antigen and then being detected using a detection system with a marker molecule. The tracer system varies from antiglobulins labelled with isotopes [74-76, 78, 89-95] to fluorochromes [96][97][98][99][100][101][102][103][104][105][106][107][108][109][110] to enzymes (described initially by Carlsson et al, 1976 [111] and reviewed by Nielsen and Gall, 1994 [112-141].…”

Section: Indirect Formatsmentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

110

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Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis, and orchitis are the most common clinical manifestations in animals. In humans, brucellosis is a debilitating and chronic disease, which may affect a variety of organs. Clinical diagnosis of brucellosis is not easily achieved. Laboratory testing is therefore very important for a correct identification of the disease in humans and for the detection and confirmation in animals. Definitive diagnosis is normally done by isolation and identification of the causative agent. While definitive, isolation is time-consuming, must be performed by highly skilled personnel, and it is hazardous. For these reasons, serological tests are normally preferred. Brucellosis serology have advanced considerably in the last decades with very sensitive and specific new tests available. Modern genetic characterization of Brucellae using molecular DNA technology have been developed. Several PCR-based assays have been proposed, from the rapid recognition of genus to differential identification of species and strains. This review describes bacteriological, serological, and molecular methods used for the diagnosis of human and animal brucellosis.

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“…SAT is the primary test used in many clinical laboratories. Although tests such as IFA and ELISA are simple and reliable for the detection of immunoglobulin (Ig) classes especially in complicated cases (3,9,14,16), many laboratories still use the classical Coombs test, as an extension of SAT, to detect "incomplete," "blocking," or "nonagglutinating" antibrucella antibodies, such as IgG (8,10,12).…”

mentioning

confidence: 99%

Evaluation of the PANBIO Brucella Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Brucellosis

Araj

Kattar

Fattouh

et al. 2005

Clin Vaccine Immunol

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Comparison of the indirect fluorescent antibody test with agglutination, complement-fixation, and Coombs tests forBrucellaantibody

Cited by 24 publications

References 3 publications

Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings

Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings

Diagnosis of Brucellosis

Evaluation of the PANBIO Brucella Immunoglobulin G (IgG) and IgM Enzyme-Linked Immunosorbent Assays for Diagnosis of Human Brucellosis

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