Comparison of the lateral tail vein and the retro-orbital venous sinus routes of antibody administration in pharmacokinetic studies

Schoch, Angela; Thorey, Irmgard S.; Engert, Julia; Winter, Gerhard; Emrich, Thomas

doi:10.1038/laban.481

Cited by 20 publications

(21 citation statements)

References 8 publications

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“…This approach is less challenging than tail vein injections and reduces the distress in the animals. 51, 52 Moreover, it has been shown that the two routes of administration can be used interchangeably and are equally effective. 53, 54 …”

Section: Methodsmentioning

confidence: 99%

Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model

Dréau

Moore

Alvarez-Berríos

et al. 2016

j biomed nanotechnol

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Mucin-1 (MUC1), a transmembrane glycoprotein is aberrantly expressed on ~90% of breast cancer and is an excellent target for nanoparticulate targeted imaging. In this study, the development of a dye-doped NIR emitting mesoporous silica nanoparticles platform conjugated to tumor-specific MUC1 antibody (ab-tMUC1-NIR-MSN) for in vivo optical detection of breast adenocarcinoma tissue is reported. The structural properties, the in vitro and in vivo performance of this nanoparticle-based probe were evaluated. In vitro studies showed that the MSN-based optical imaging nanoprobe is non-cytotoxic and targets efficiently mammary cancer cells overexpressing human tMUC1 protein. In vivo experiments with female C57BL/6 mice indicated that this platform accumulates mainly in the liver and did not induce short-term toxicity. In addition, we demonstrated that the ab-tMUC1-NIR-MSN nanoprobe specifically detects mammary gland tumors overexpressing human tMUC1 in a human MUC1 transgenic mouse model.

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Section: Methodsmentioning

confidence: 99%

Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model

Dréau

Moore

Alvarez-Berríos

et al. 2016

j biomed nanotechnol

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“…As mentioned above, other studies have been published comparing the TV and RO administration routes that show similar results when delivering drugs, antibodies or cells in similar situations. [2][3][4][5][6]9 Also, in our hands, RO administration has shown greater consistency regarding donor chimerism levels due to the ease of the technique and the lower rate of failure.…”

Section: Discussionmentioning

confidence: 65%

Comparison of haematopoietic stem cell engraftment through the retro-orbital venous sinus and the lateral vein: alternative routes for bone marrow transplantation in mice

León-Rico¹,

Fernández-García²,

Aldea³

et al. 2015

Lab Anim

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Bone marrow transplantation in mice is performed by intravenous administration of haematopoietic repopulating cells, usually via the lateral tail vein. This technique can be technically challenging to carry out and may cause distress to the mice. The retro-orbital sinus is a large area where there is a confluence of several vessels that provides an alternative route for intravenous access. Retro-orbital injection, although aesthetically unpleasant, can be performed rapidly without requiring mechanical restriction or heat-induced vasodilation. In addition, this technique can be easily learned by novice manipulators. This route of administration has been reported for use in bone marrow transplantation but there is no comparison of retro-orbital and tail vein injections reported for this specific purpose, although both routes have been compared for many other applications. Here, we provide for the first time a comprehensive comparison between tail vein and retro-orbital injections for two different bone marrow transplant scenarios in P3B and B6D2F1 mice. In both cases, no significant differences regarding donor engraftment were observed between mice transplanted using each of the techniques. Haematological counts and leukocyte subpopulation distribution were practically identical between both animal groups. Moreover, donor engraftment levels were less homogenous when cells were transplanted by tail vein injection, probably due to a higher risk of failure associated with this technique. All these data suggest that retro-orbital injection is a compelling alternative to conventional tail vein injection for bone marrow transplant in mice, providing similar and more homogenous haematopoietic reconstitution.

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“…After mice anesthesia, we carefully inserted the needle into mouse medial canthus at an angle of approximately 30°, and slowly push the solution into retro-orbital venous sinus; the detail procedure of retro-orbital injection is shown in 31 . Comparison with the common-used lateral tail vein injection, retro-orbital injection provides higher rate of successful injection that can decrease the stress of mice and there is no difference of pharmacokinetics via both two intravenous injections 32 , 33 . After waiting for 10 min for thorough distribution of RBCMDs in the blood circulation, the right leg of the mice was insonated with 3.5-MHz and 3-cycle HIFU (SU-107, Sonic Concepts, WA, USA) at an acoustic pressure of 8 MPa (peak rarefactional pressure) at 10 pulses/s for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Biomimetic Acoustically-Responsive Vesicles for Theranostic Applications

Hsieh¹,

Kang²,

Lin³

et al. 2015

Theranostics

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In recent years, biomimetic cell membrane-derived particles have emerged as a new class of drug delivery system with advantages of biocompatibility, ease of isolation and long circulation profile. Here we report the development and potential theranostic applications of a new biomimetic acoustically-responsive droplet system derived from mammalian red blood cell membrane (RBCM). We hypothesized that drug-loaded RBCM droplets (RBCMDs) would undergo a transition from liquid (droplets) to gas (bubbles) upon high intensity focused ultrasound (HIFU) insonation, resulting in on-demand drug release. The generated microbubbles could also serve as a contrast agent to enhance ultrasound imaging. As-synthesized RBCMDs exhibited uniform size, good dispersity and preservation of RBCM-associated proteins that prevented uptake by macrophages. Camptothecin (CPT), an anti-cancer drug, was successfully loaded in the RBCMDs with a loading efficiency of 2-3% and an encapsulation efficiency of 62-97%. A short (3 min) exposure to HIFU irradiation triggered release of CPT from the RBCMDs and the physical explosion of droplets damaged nearby cancer cells resulting in significant cell death. In addition, the acoustically vaporized RBCMDs significantly increased the ultrasound echo signal to 30 dB. Lastly, we demonstrated that RBCMDs could be acoustically vaporized in vivo in target tissues, and enhancing ultrasound imaging. Taken together, we have developed a new class of naturally derived RBCMDs which show great potential for future application in remotely triggered drug delivery and ultrasound imaging enhancement.

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Comparison of the lateral tail vein and the retro-orbital venous sinus routes of antibody administration in pharmacokinetic studies

Cited by 20 publications

References 8 publications

Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model

Mucin-1-Antibody-Conjugated Mesoporous Silica Nanoparticles for Selective Breast Cancer Detection in a Mucin-1 Transgenic Murine Mouse Model

Comparison of haematopoietic stem cell engraftment through the retro-orbital venous sinus and the lateral vein: alternative routes for bone marrow transplantation in mice

Biomimetic Acoustically-Responsive Vesicles for Theranostic Applications

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