Comparison of the Luminex Respiratory Virus Panel Fast Assay with In-House Real-Time PCR for Respiratory Viral Infection Diagnosis

Gadsby, Naomi J.; Hardie, Alison; Claas, Eric C. J.; Templeton, Kate

doi:10.1128/jcm.02446-09

Cited by 117 publications

(123 citation statements)

References 16 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…Here, introduction of these testing modalities has resulted in markedly higher rates of detection, increased numbers of samples positive for multiple pathogens, and an ability to more fully characterize the epidemiology of disease. 6,9,11,18,19 Detection of gastrointestinal pathogens could have a similar impact, with an ability to detect pathogens (particularly viruses) not previously tested for by routine methods, and with some investigators reporting increased sensitivity for other pathogens now routinely detected through conventional methods. 15 Reduced time to detection and processing time could also prove beneficial.…”

Section: Discussionmentioning

confidence: 96%

Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients

Zhu

Rodríguez

et al. 2015

The Journal of Molecular Diagnostics

View full text Add to dashboard Cite

Broadly multiplexed molecular amplification assays offer an unprecedented ability to diagnose gastrointestinal infection in immunocompromised patients. However, little data are available to compare the performance of such systems in this population. A total of 436 stool samples were collected from 199 predominantly immunocompromised pediatric oncology patients. Remnant samples were tested in parallel with the use of the premarket (investigational use only) versions of two broadly multiplexed PCR assays (BioFire and Luminex), and the results of samples corresponding to the first episode per patient were compared with those from laboratory-developed molecular assays, culture, and antigen detection. Overall performance of the multiplexed systems was comparable, with BioFire and Luminex detecting 94 and 99 positives (P = 0.34), respectively. Stratifying by analyte, BioFire assay detected 51 samples positive for Clostridium difficile, whereas Luminex assay detected 60 (P = 0.01). Biofire and Luminex detected 28 and 38 norovirus-positive samples (P = 0.002), respectively. Astrovirus- and adenovirus-positive samples were detected in higher numbers by in-house PCR than by BioFire; the same was observed for adenovirus with Luminex. Differences observed with other analytes were minimal, did not reach statistical significance, or lacked the numbers needed to detect a difference between systems. Broadly multiplexed PCR offers an effective means of detecting a variety of gastrointestinal pathogens in pediatric oncology patients, with assay performance comparable among the tests examined.

show abstract

Section: Discussionmentioning

confidence: 96%

Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients

Zhu

Rodríguez

et al. 2015

The Journal of Molecular Diagnostics

View full text Add to dashboard Cite

show abstract

“…These findings suggest that the discordant results were frequently reported in samples containing low viral copy. Other studies also indicated that discrepant results between RV assay was quite associated with sample's viral load 7, 8, 19, 20, 21. One samples by AP and three samples by AD identified as INF A positive by additional testing.…”

Section: Discussionmentioning

confidence: 97%

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Yun

Kim

Choi

et al. 2017

Clinical Laboratory Analysis

View full text Add to dashboard Cite

BackgroundDue to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT‐PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT‐PCR assays for the detection of respiratory viruses.MethodsA total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real‐Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence‐adjusted and bias‐adjusted kappa (PABAK) values was performed for comparisons among the three RV assays.ResultsOf the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%‐98%, 0.76‐0.86, and 0.93‐0.96 respectively. The performance of the three assays was very similar, with 94%‐100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results.ConclusionsWe suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

show abstract

“…The nasopharyngeal swabs of these children, which had been collected at the time of admission for the routine screening of respiratory infectious agents and kept in a freezer at −80 • C, were retrieved for the identification of EV-D68. Our Unit routinely screens the nasopharyngeal swabs of children with CAP for Streptococcus pneumoniae [11] and viruses using the RVP Fast assay (Luminex Molecular Diagnostics Inc., Toronto, Canada), which simultaneously detects 17 viruses including enterovirus/rhinovirus [12].…”

Section: Methodsmentioning

confidence: 99%

Enterovirus D68-associated community-acquired pneumonia in children living in Milan, Italy

Esposito

Zampiero

Ruggiero

et al. 2015

Journal of Clinical Virology

View full text Add to dashboard Cite

Comparison of the Luminex Respiratory Virus Panel Fast Assay with In-House Real-Time PCR for Respiratory Viral Infection Diagnosis

Cited by 117 publications

References 16 publications

Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients

Comparative Evaluation of Broad-Panel PCR Assays for the Detection of Gastrointestinal Pathogens in Pediatric Oncology Patients

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Enterovirus D68-associated community-acquired pneumonia in children living in Milan, Italy

Contact Info

Product

Resources

About