Comparison of the metabolomic and proteomic profiles associated with triterpene and phytosterol accumulation between wild and cultivated ginseng

Ma, Rui; Yang, Pengdi; Jing, Chuanyong; Fu, Baoyu; Teng, Xiaoyu; Zhao, Daqing; Sun, Liwei

doi:10.1016/j.plaphy.2023.01.020

Cited by 15 publications

(5 citation statements)

References 37 publications

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“…34,35 SE is also a critical gene in the saponin biosynthesis pathway of Panax notoginseng, Eleutherococcus Senticosus, and ginseng. [36][37][38] In this study, significantly higher levels of total saponins and Platycodin D in 1-year-old seedlings was observed compared to their 6-monthold counterparts. These results can be explained by the higher expression levels of key genes associated with the triterpenoid saponin synthesis pathway in 1-year-old seedlings, which regulate the essential pathway of saponin synthesis.…”

Section: The Content Of Platycodin Dsupporting

confidence: 52%

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Liu,

Ren,

Wang

et al. 2023

Natural Product Communications

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Objective: To investigate the impact of developmental stages on triterpene saponin content and expression of key genes in their biosynthetic pathway in Platycodon grandiflorus seedlings. Method: The total saponins were extracted using ultrasonic extraction with methanol as the solvent. The quantification of Platycodin D in 6-month-old and 1-year-old Platycodon grandiflorus seedlings was performed using ultra-performance liquid chromatography tandem mass spectrometry, while the expression of key genes in the biosynthesis of saponins was determined by real-time quantitative polymerase chain reaction. Result: The content of Platycodin D and total saponins in 1-year-old Platycodon grandiflorus roots was significantly higher than that in of 6-month-old. The expression of key genes in saponin biosynthesis, such as PGMVK , PGMVD , PGDXR , PGMCT , PGMDS , PGHDS , PGHDR , PGFPS, and PGSS, was higher in 1-year-old roots compared to 6-month-old seedlings. Conclusion: The medicinal value of 1-year-old Platycodon grandiflorus root was higher than that of 6-month-old seedlings. The difference in medicinal value can be attributed to the variation in the expression of key genes involved in the saponin biosynthesis pathway.

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Section: The Content Of Platycodin Dsupporting

confidence: 52%

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Liu,

Ren,

Wang

et al. 2023

Natural Product Communications

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“…Next, a Student’s t -test was performed to identify metabolites, showing notable variances between the BS and control treatments across all phases. In terms of metabolic pathway assessment, pathways meeting the criteria of p < 0.05 in the hypergeometric test and pathway impact ≥ 0.1 were regarded as disrupted structures [ 29 ]. In order to reconstruct the metabolic pathways, a two-way analysis of variance (ANOVA) was utilized to compare the averages of each metabolite or protein among various treatments and stages.…”

Section: Methodsmentioning

confidence: 99%

Changes of Active Substances in Ganoderma lucidum during Different Growth Periods and Analysis of Their Molecular Mechanism

Gao,

Huo,

Bao

et al. 2024

Molecules

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Ganoderma lucidum, renowned as an essential edible and medicinal mushroom in China, remains shrouded in limited understanding concerning the intrinsic mechanisms governing the accumulation of active components and potential protein expression across its diverse developmental stages. Accordingly, this study employed a meticulous integration of metabolomics and proteomics techniques to scrutinize the dynamic alterations in metabolite accumulation and protein expression in G. lucidum throughout its growth phases. The metabolomics analysis unveiled elevated levels of triterpenoids, steroids, and polyphenolic compounds during the budding stage (BS) of mushroom growth, with prominent compounds including Diplazium and Ganoderenic acids E, H, and I, alongside key steroids such as cholesterol and 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol. Additionally, nutrients such as polysaccharides, flavonoids, and purines exhibited heightened presence during the maturation stage (FS) of ascospores. Proteomic scrutiny demonstrated the modulation of triterpenoid synthesis by the CYP450, HMGR, HMGS, and ERG protein families, all exhibiting a decline as G. lucidum progressed, except for the ARE family, which displayed an upward trajectory. Therefore, BS is recommended as the best harvesting period for G. lucidum. This investigation contributes novel insights into the holistic exploitation of G. lucidum.

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“…Due to artificial breeding or changes in the living conditions of medicinal plants, such as climate change, the abuse of fertilizers and pesticides or environmental pollution, some medicinal plants have experienced the degradation of traits and qualities. For instance, the biosynthesis of ginsenosides and phytosterols in wild ginseng is higher than that in cultivated ginseng, as well as the ginsenoside diversity ( Ma et al, 2023 ). By measuring and improving the relative abundance of rhizosphere microorganisms related to the biosynthesis of medicinal components, it is of great value to ensure the effectiveness of medicinal plants.…”

Section: Role and Significance Of Micro-markersmentioning

confidence: 99%

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

Yang,

Zheng,

Sun

et al. 2024

Chinese Herbal Medicines

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Comparison of the metabolomic and proteomic profiles associated with triterpene and phytosterol accumulation between wild and cultivated ginseng

Cited by 15 publications

References 37 publications

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Effect of Developmental Stages on the Biosynthesis of Triterpene Saponins and Related Gene Expression in Seedlings of Platycodon grandiflorus

Changes of Active Substances in Ganoderma lucidum during Different Growth Periods and Analysis of Their Molecular Mechanism

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

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