Comparison of the microbial community composition of pristine rock cores and technical influenced well fluids from the Ketzin pilot site for CO2 storage

Pellizzari, Linda; Morozova, Daria; Neumann, Dominik; Kasina, Monika; Klapperer, Stefan; Zettlitzer, Michael; Würdemann, Hilke

doi:10.1007/s12665-016-6111-6

Cited by 8 publications

(4 citation statements)

References 73 publications

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“…Several studies focused on hydrochemical and geophysical features of wet mofettes (Pfanz, 2008 ; Kämpf et al, 2013 ), but biological processes were so far not considered. Most knowledge on environmental microbial communities in habitats with elevated CO 2 concentrations were either gained from dry mofettes or soils (Krüger et al, 2009 , 2011 ; Beulig et al, 2016 ), Carbon Capture and Storage (CCS) sites (Morozova et al, 2010 ; Wandrey et al, 2011a , b ; Pellizzari et al, 2016 ) and deep subsurface fluids (Emerson et al, 2016 ; Probst et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiological and Geochemical Survey of CO2-Dominated Mofette and Mineral Waters of the Cheb Basin, Czech Republic

et al. 2017

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The Cheb Basin (NW Bohemia, Czech Republic) is a shallow, neogene intracontinental basin. It is a non-volcanic region which features frequent earthquake swarms and large-scale diffuse degassing of mantle-derived CO2 at the surface that occurs in the form of CO2-rich mineral springs and wet and dry mofettes. So far, the influence of CO2 degassing onto the microbial communities has been studied for soil environments, but not for aquatic systems. We hypothesized, that deep-trenching CO2 conduits interconnect the subsurface with the surface. This admixture of deep thermal fluids should be reflected in geochemical parameters and in the microbial community compositions. In the present study four mineral water springs and two wet mofettes were investigated through an interdisciplinary survey. The waters were acidic and differed in terms of organic carbon and anion/cation concentrations. Element geochemical and isotope analyses of fluid components were used to verify the origin of the fluids. Prokaryotic communities were characterized through quantitative PCR and Illumina 16S rRNA gene sequencing. Putative chemolithotrophic, anaerobic and microaerophilic organisms connected to sulfur (e.g., Sulfuricurvum, Sulfurimonas) and iron (e.g., Gallionella, Sideroxydans) cycling shaped the core community. Additionally, CO2-influenced waters form an ecosystem containing many taxa that are usually found in marine or terrestrial subsurface ecosystems. Multivariate statistics highlighted the influence of environmental parameters such as pH, Fe2+ concentration and conductivity on species distribution. The hydrochemical and microbiological survey introduces a new perspective on mofettes. Our results support that mofettes are either analogs or rather windows into the deep biosphere and furthermore enable access to deeply buried paleo-sediments.

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Section: Discussionmentioning

confidence: 99%

Microbiological and Geochemical Survey of CO2-Dominated Mofette and Mineral Waters of the Cheb Basin, Czech Republic

et al. 2017

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“…D'Hondt et al 2004;Parkes et al 2005) was detected in well fluids from a depth of 647 m that were influenced by organic drilling mud (Morozova et al 2011). Fluorescent in situ hybridization analyses revealed a high abundance of sulphate-reducing bacteria in these fluids (Pellizzari et al 2016). Furthermore, fermentative halophilic bacteria, which were related to species of Halanaerobium, and sulphate-reducing bacteria distantly related to species of Desulfohalobium were detected by genetic fingerprinting.…”

Section: Effect Of Borefluidmentioning

confidence: 97%

Mineralogical and geochemical analysis of Fe-phases in drill-cores from the Triassic Stuttgart Formation at Ketzin CO2 storage site before CO2 arrival

et al. 2017

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Reactive iron (Fe) oxides and sheet silicatebound Fe in reservoir rocks may affect the subsurface storage of CO 2 through several processes by changing the capacity to buffer the acidification by CO 2 and the permeability of the reservoir rock: (1) the reduction of threevalent Fe in anoxic environments can lead to an increase in pH, (2) under sulphidic conditions, Fe may drive sulphur cycling and lead to the formation of pyrite, and (3) the leaching of Fe from sheet silicates may affect silicate diagenesis. In order to evaluate the importance of Fe-reduction on the CO 2 reservoir, we analysed the Fe geochemistry in drill-cores from the Triassic Stuttgart Formation (Schilfsandstein) recovered from the monitoring well at the CO 2 test injection site near Ketzin, Germany.The reservoir rock is a porous, poorly to moderately cohesive fluvial sandstone containing up to 2-4 wt% reactive Fe. Based on a sequential extraction, most Fe falls into the dithionite-extractable Fe-fraction and Fe bound to sheet silicates, whereby some Fe in the dithionite-extractable Fe-fraction may have been leached from illite and smectite. Illite and smectite were detected in core samples by X-ray diffraction and confirmed as the main Fe-containing mineral phases by X-ray absorption spectroscopy. Chlorite is also present, but likely does not contribute much to the high amount of Fe in the silicate-bound fraction. The organic carbon content of the reservoir rock is extremely low (\0.3 wt%), thus likely limiting microbial Fe-reduction or sulphate reduction despite relatively high concentrations of reactive Fe-mineral phases in the reservoir rock and sulphate in the reservoir fluid. Both processes could, however, be fuelled by organic matter that is mobilized by This article is part of a Topical Collection in Environmental Earth Sciences on ''Subsurface Energy storage'', guest-edited by

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“…Growing concerns about global warming due to increasing atmospheric CO 2 concentrations bring attention towards Carbon capture and storage (CCS), an engineering solution to limit the release of CO 2 into the atmosphere by capturing the CO 2 at its source and storing it in suitable underground reservoirs, i.e., deep saline aquifers or depleted hydrocarbon reservoirs. Demonstration projects such as CO2SINK in Ketzin, Germany and FutureGen in Illinois, United States provide scientists the opportunity to study the impact of CCS on the surrounding environment, including microbial communities living in these ecosystems (Morozova et al, 2011;Gilmore et al, 2014;Pellizzari et al, 2016). However, until now the majority of CCS facilities have only been operated for a relatively short time period, leaving the longterm effects of elevated CO 2 concentrations in subsurface environments and thus long-term stability of CCS largely unclear.…”

Section: Introductionmentioning

confidence: 99%

Enrichment of rare methanogenic Archaea shows their important ecological role in natural high-CO2 terrestrial subsurface environments

et al. 2023

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IntroductionLong-term stability of underground CO2 storage is partially affected by microbial activity but our knowledge of these effects is limited, mainly due to a lack of sites. A consistently high flux of mantle-derived CO2 makes the Eger Rift in the Czech Republic a natural analogue to underground CO2 storage. The Eger Rift is a seismically active region and H2 is produced abiotically during earthquakes, providing energy to indigenous microbial communities.MethodsTo investigate the response of a microbial ecosystem to high levels of CO2 and H2, we enriched microorganisms from samples from a 239.5 m long drill core from the Eger Rift. Microbial abundance, diversity and community structure were assessed using qPCR and 16S rRNA gene sequencing. Enrichment cultures were set up with minimal mineral media and H2/CO2 headspace to simulate a seismically active period with elevated H2.Results and discussionMethane headspace concentrations in the enrichments indicated that active methanogens were almost exclusively restricted to enrichment cultures from Miocene lacustrine deposits (50–60 m), for which we observed the most significant growth. Taxonomic assessment showed microbial communities in these enrichments to be less diverse than those with little or no growth. Active enrichments were especially abundant in methanogens of the taxa Methanobacterium and Methanosphaerula. Concurrent to the emergence of methanogenic archaea, we also observed sulfate reducers with the metabolic ability to utilize H2 and CO2, specifically the genus Desulfosporosinus, which were able to outcompete methanogens in several enrichments. Low microbial abundance and a diverse non-CO2 driven microbial community, similar to that in drill core samples, also reflect the inactivity in these cultures. Significant growth of sulfate reducing and methanogenic microbial taxa, which make up only a small fraction of the total microbial community, emphasize the need to account for rare biosphere taxa when assessing the metabolic potential of microbial subsurface populations. The observation that CO2 and H2-utilizing microorganisms could only be enriched from a narrow depth interval suggests that factors such as sediment heterogeneity may also be important. This study provides new insight on subsurface microbes under the influence of high CO2 concentrations, similar to those found in CCS sites.

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Comparison of the microbial community composition of pristine rock cores and technical influenced well fluids from the Ketzin pilot site for CO2 storage

Abstract: Exter Formation rocks, Proteobacteria and Actinobacteria were detected. These data provide an explicit reference to further evaluate environmental changes and community shifts in the reservoir during CO2 storage and provide information for evaluating the storage efficiency and reliability.

Cited by 8 publications

References 73 publications

Microbiological and Geochemical Survey of CO2-Dominated Mofette and Mineral Waters of the Cheb Basin, Czech Republic

Microbiological and Geochemical Survey of CO2-Dominated Mofette and Mineral Waters of the Cheb Basin, Czech Republic

Mineralogical and geochemical analysis of Fe-phases in drill-cores from the Triassic Stuttgart Formation at Ketzin CO2 storage site before CO2 arrival

Enrichment of rare methanogenic Archaea shows their important ecological role in natural high-CO2 terrestrial subsurface environments

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