Comparison of the Microbiota and Inorganic Anion Content in the Saliva of Patients with Gastroesophageal Reflux Disease and Gastroesophageal Reflux Disease-Free Individuals

Ziganshina, Elvira E.; Sagitov, Ildar I.; Akhmetova, Ramilya F.; Saleeva, G T; Kiassov, A.; Gogoleva, Natalya E.; Shagimardanova, Elena; Ziganshin, Ayrat M.

doi:10.1155/2020/2681791

Cited by 15 publications

(10 citation statements)

References 40 publications

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“…Bicarbonate level influences salivary pH and pH increase as bicarbonate concentration increase, but non-stimulated saliva insufficiently buffered because bicarbonate level is too low to be effective [32]. Based on the present results, no significant difference was found in salivary phosphate between established groups and this result was agreed with other studies [33].…”

Section: Laboratory Findingssupporting

confidence: 91%

Untitled

2023

J. Med. Chem. Sci.

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The neutralization of stomach's acidic contents and protection of esophageal mucous membrane during reflux episodes is the primary function of the major buffering components of saliva, bicarbonate, and phosphate. The concentrations of inorganic compounds in the saliva of subjects who have Gastroesophageal reflux disease versus healthy individuals are poorly understood. In this work, the aim of study was to assess the occurrence of soft tissues alterations and symptoms in oral cavity and analysis of bicarbonate and phosphate to evaluate their levels in saliva of patients with GERD and compare with control individuals. A case-control study included 25 GERD patients diagnosed both clinically and endoscopically (GERD Group), 25 patients with upper gastrointestinal symptoms with no endoscopic signs of GERD (non GERD group) and 25 healthy subjects (control group) have been evaluated through visual examination of the oral mucosa, oral symptoms, nonstimulated salivary flow rate, swallowing function, in addition to the estimation of salivary concentration of bicarbonate and phosphate. Study result was significantly higher salivary bicarbonate in GERD patients (p<0.05), significantly lower Salivary flow rate and swallowing frequency compared to non-GERD group and control group. Among the patients with GERD, 56% of the cases suffering from oral dryness, 40% halitosis, 28% acid taste, 20% burning sensation, 20% palatal erythema. Salivary bicarbonate, oral symptom and mucosal alteration were shown to be significantly related to GERD. Therefore, GERD should be taken into account by dentists as a possible risk factor for oral health K E Y W O R D S Gastroesophageal reflux disease Oral symptoms Salivary flow rate Salivary bicarbonate Salivary phosphate

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Section: Laboratory Findingssupporting

confidence: 91%

Untitled

2023

J. Med. Chem. Sci.

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“…Several studies have identified increased abundances of the oral microbe Fusobacterium in setting of colorectal cancer ( 29 , 33 – 35 ), liver cirrhosis ( 36 – 38 ), primary sclerosing cholangitis ( 39 – 41 ), gastroesophageal reflux disease ( 42 – 46 ), HIV infection ( 47 – 49 ), alcoholism ( 50 ), and IBD ( 6 , 10 – 19 ). Given the prevalence of F. nucleatum in mucosal specimens, we tested the hypothesis that this pathobiont could promote an epithelial proinflammatory response and potentially contribute to intestinal inflammation.…”

Section: Resultsmentioning

confidence: 99%

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Engevik

Danhof

Ruan

et al. 2021

mBio

154

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Multiple studies have implicated microbes in the development of inflammation, but the mechanisms remain unknown. Bacteria in the genus Fusobacterium have been identified in the intestinal mucosa of patients with digestive diseases; thus, we hypothesized that Fusobacterium nucleatum promotes intestinal inflammation. The addition of >50 kDa F. nucleatum conditioned media, which contain outer membrane vesicles (OMVs), to colonic epithelial cells stimulated secretion of the proinflammatory cytokines interleukin-8 (IL-8) and tumor necrosis factor (TNF). In addition, purified F. nucleatum OMVs, but not compounds <50 kDa, stimulated IL-8 and TNF production; which was decreased by pharmacological inhibition of Toll-like receptor 4 (TLR4). These effects were linked to downstream effectors p-ERK, p-CREB, and NF-κB. F. nucleatum >50-kDa compounds also stimulated TNF secretion, p-ERK, p-CREB, and NF-κB activation in human colonoid monolayers. In mice harboring a human microbiota, pretreatment with antibiotics and a single oral gavage of F. nucleatum resulted in inflammation. Compared to mice receiving vehicle control, mice treated with F. nucleatum showed disruption of the colonic architecture, with increased immune cell infiltration and depleted mucus layers. Analysis of mucosal gene expression revealed increased levels of proinflammatory cytokines (KC, TNF, IL-6, IFN-γ, and MCP-1) at day 3 and day 5 in F. nucleatum-treated mice compared to controls. These proinflammatory effects were absent in mice who received F. nucleatum without pretreatment with antibiotics, suggesting that an intact microbiome is protective against F. nucleatum-mediated immune responses. These data provide evidence that F. nucleatum promotes proinflammatory signaling cascades in the context of a depleted intestinal microbiome. IMPORTANCE Several studies have identified an increased abundance of Fusobacterium in the intestinal tracts of patients with colon cancer, liver cirrhosis, primary sclerosing cholangitis, gastroesophageal reflux disease, HIV infection, and alcoholism. However, the direct mechanism(s) of action of Fusobacterium on pathophysiological within the gastrointestinal tract is unclear. These studies have identified that F. nucleatum subsp. polymorphum releases outer membrane vesicles which activate TLR4 and NF-κB to stimulate proinflammatory signals in vitro. Using mice harboring a human microbiome, we demonstrate that F. nucleatum can promote inflammation, an effect which required antibiotic-mediated alterations in the gut microbiome. Collectively, these results suggest a mechanism by which F. nucleatum may contribute to intestinal inflammation.

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“…Amplicon sequencing was conducted using an Illumina MiSeq system with 2 × 300 bp reads. The sequence data were then analyzed as detailed previously [ 15 , 20 , 21 , 22 ] and available on request.…”

Section: Methodsmentioning

confidence: 99%

Anaerobic Digestion of Chicken Manure in the Presence of Magnetite, Granular Activated Carbon, and Biochar: Operation of Anaerobic Reactors and Microbial Community Structure

Ziganshina

Ziganshin

2022

Microorganisms

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The influence of magnetite nanoparticles, granular activated carbon (GAC), and biochar, as well as their combinations on the anaerobic digestion of chicken manure and the structure of microbial communities was studied. The addition of magnetite, GAC, and biochar increased the rate of methane production and the total methane yield. It has been observed that these additives stimulated anaerobic microorganisms to reduce the concentration of accumulated volatile organic acids. Various bacterial species within the classes Bacteroidia and Clostridia were found at higher levels in the anaerobic reactors but in different proportions depending on the experiment. Members of the genera Methanosarcina, Methanobacterium, Methanothrix, and Methanoculleus were mainly detected within the archaeal communities in the anaerobic reactors. Compared to the 16S rRNA gene-based study, the mcrA gene approach allowed a higher level of Methanosarcina in the system with GAC + magnetite to be detected. Based on our findings, the combined use of granular activated carbon and magnetite at appropriate dosages will improve biomethane production.

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Comparison of the Microbiota and Inorganic Anion Content in the Saliva of Patients with Gastroesophageal Reflux Disease and Gastroesophageal Reflux Disease-Free Individuals

Cited by 15 publications

References 40 publications

Untitled

Untitled

Fusobacterium nucleatum Secretes Outer Membrane Vesicles and Promotes Intestinal Inflammation

Anaerobic Digestion of Chicken Manure in the Presence of Magnetite, Granular Activated Carbon, and Biochar: Operation of Anaerobic Reactors and Microbial Community Structure

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