Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci

Hussain, Zafar; Stoakes, Luba; Stevens, Donald L.; Schieven, Berend C.; Lannigan, R.; Jones, C

doi:10.1128/jcm.23.1.126-128.1986

Cited by 27 publications

(15 citation statements)

References 7 publications

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“…The advent of identification systems that rely on the detection of performance enzymes and use substrates that cannot be used in the conventional identification methods, it is now possible to achieve accurate same-day identification of Staphylococcus species (HUSSAIN et al 1986).…”

Section: Fig 1 % Of Discord Between Biochemical Tests To Microscan Wmentioning

confidence: 99%

Evaluation of the MicroScan system for identification of staphylococci

Saa¹,

Vivas²,

Tinajas³

et al. 1999

J. Basic Microbiol.

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Conventional biochemical tests were compared with reactions in a multiple test system, MicroScan Walkaway (Dade Diagnostic Inc. MicroScan Divison, West Sacramento, California) in conjugation with the Combo Pos ID Panels (Dade Diagnostic Inc. MicroScan Divison, West Sacramento, California), in order to evaluate the accuracy for the identification of 99 clinical isolates of Staphylococcus spp. and five reference strains. False-negative or positive reactions were detected from Voges-Proskauer, urease and mannose tests. A good correlation was found among the two identification systems for the fermentation of trehalose, lactose, raffinose, as well as for arginine dyhydrolase, esculin hydrolisis and nitrate reduction. From the results of the present study, it is concluded that the MicroScan Walkaway system is a reliable method for identification of staphylococci (94.23%), although 8.2% could be identified to the species level only after use of additional test.

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Section: Fig 1 % Of Discord Between Biochemical Tests To Microscan Wmentioning

confidence: 99%

Evaluation of the MicroScan system for identification of staphylococci

Saa¹,

Vivas²,

Tinajas³

et al. 1999

J. Basic Microbiol.

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“…Several manual and automated methods for the identification of staphylococci are commercially available (6,27). Since rapid systems or methods for the specific identification of S. epidermidis directly from clinical specimens are not available, this species and other CoNS can only be identified from bacterial cultures with systems based on biochemical tests which require from 4 to 48 h of incubation (2,(8)(9)(10). Furthermore, commercially available panels, which are based on functional differences in metabolic pathways, do not allow for the reliable distinction of S. epidermidis from other CoNS (21).…”

mentioning

confidence: 99%

Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis

et al. 1996

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Staphylococcus epidermidis is an aerobic gram-positive coccus that is now recognized among the coagulasenegative staphylococci as an etiological agent with an important range of pathogenicity in humans. Several diagnostic kits based on biochemical or immunological reactions can efficiently identify Staphylococcus aureus. However, these tests are often unreliable for the identification of coagulase-negative staphylococcal species including S. epidermidis. Since DNA-based assays for the species-specific identification of S. epidermidis remain unavailable, we have developed such tests in order to improve the accuracy and the rapidity of tests for the diagnosis of S. epidermidis infections. On the basis of the results of hybridization assays with clones randomly selected from an S. epidermidis genomic library, we identified a chromosomal DNA fragment which is specific and 100% ubiquitous for the identification of S. epidermidis. This 705-bp fragment was sequenced and used to design PCR amplification primers. PCR assays with the selected primers were also highly specific and ubiquitous for the identification from bacterial cultures of clinical isolates of S. epidermidis from a variety of anatomic sites. While three strains of S. capitis were misidentified as S. epidermidis with the API Staph-Ident system and 2.5% of the S. epidermidis identifications were inconclusive with the MicroScan Autoscan-4 system, the PCR assay was highly specific and allowed for the correct identification of all 79 S. epidermidis strains tested. The PCR assays developed are simple and can be performed in about 1 h. These DNA-based tests provide novel diagnostic tools for improving the diagnosis of S. epidermidis infections.

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“…The identification of the less common species is more variable. Identification of some isolates of S. hominis and S. warneri has proven to be problematic with most of the above systems (13,107,108,122,192,239). The MS Rapid GP system, which requires 2 h for incubation, provides a level of accurate identification similar to MS GP, Vitek GPI, and Staph ID 32, which require 4 to 24 h of incubation (Table 7).…”

Section: Micrococcaceaementioning

confidence: 99%

Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci

Bascomb¹,

Manafi

1998

Clin Microbiol Rev

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SUMMARY The contribution of enzyme tests to the accurate and rapid routine identification of gram-positive cocci is introduced. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. The clinical and economic importance of members of these taxa is briefly summarized. Tables summarizing test schemes and kits available for the identification of staphylococci, enterococci, and streptococci on the basis of general requirements, number of tests, number of taxa, test classes, and completion times are discussed. Enzyme tests included in each scheme are compared on the basis of their synthetic moiety. The current understanding of the activity of enzymes important for classification and identification of the major groups, methods of testing, and relevance to the ease and speed of identification are reviewed. Publications describing the use of different identification kits are listed, and overall identification successes and problems are discussed. The relationships between the results of conventional biochemical and rapid enzyme tests are described and considered. The use of synthetic substrates for the detection of glycosidases and peptidases is reviewed, and the advantages of fluorogenic synthetic moieties are discussed. The relevance of enzyme tests to accurate and meaningful rapid routine identification is discussed.

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Comparison of the MicroScan system with the API Staph-Ident system for species identification of coagulase-negative staphylococci

Cited by 27 publications

References 7 publications

Evaluation of the MicroScan system for identification of staphylococci

Evaluation of the MicroScan system for identification of staphylococci

Species-specific and ubiquitous DNA-based assays for rapid identification of Staphylococcus epidermidis

Use of Enzyme Tests in Characterization and Identification of Aerobic and Facultatively Anaerobic Gram-Positive Cocci

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