Comparison of the molecular effects of the mycotoxins β-zearalenol and deoxynivalenol in porcine endometrial cells — A review

Abstract

The mycotoxins β-zearalenol (β-ZOL) and deoxynivalenol (DON) produce toxic effects that result in diseases in humans and animals. The molecular mechanisms that control the mycotoxin-mediated effects are far from being completely understood. Various results show that these mycotoxins could inhibit cell proliferation. In the present short communication, the influence of β-ZOL and DON on the abundance and phosphorylation state of kinases that are included in regulation of the initiation of mRNA translation (which is correlated with cell proliferation) was compared in porcine endometrial cells (PEC). Our results indicate that these mycotoxins modulate the expression and phosphorylation of these factors in a different manner. Whereas β-ZOL mainly had an impact on the biological activity of the extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), protein kinase B (Akt), eukaryotic initiation factor 4E (eIF4E) and its repressor 4E binding protein 1 (4E-BP1), DON reduced the abundance of p38 MAPk, Akt and specific 4E-BP1 bands. In summary, these results indicate that β-ZOL influences molecular events that are included in the initiation of mRNA translation in the porcine endometrium but DON does not alter such processes clearly.

Effect of deoxynivalenol on apoptosis, barrier function, and expression levels of genes involved in nutrient transport, mitochondrial biogenesis and function in IPEC-J2 cells

“…Several studies have demonstrated that DON could decrease cell proliferation and reduce the viability of different cells with different concentrations. 12,25 In addition, DON can significantly inhibit the proliferation of IPEC-J2 cells. 11,19,26,27 In our study, the cell viability assay showed a similar suppressive response to 200 ng mL −1 of DON and 2000 ng mL −1 of DON at 24 h, 48 h, and 72 h (Fig.…”

Neurophysiological and behavioral effects of mycotoxin deoxynivalenol and fumonisin

“…Deoxynivalenol affects animal and human health causing acute temporary nausea, vomiting, diarrhea, abdominal pain, headache, dizziness, and fever (Sobrova et al, 2010). At the molecular level, deoxynivalenol disrupts normal cell function by inhibiting protein synthesis via binding to the ribosome and by activating critical cellular kinases involved in signal transduction related to proliferation, differentiation and apoptosis (Wollenhaupt et al, 2007;Abdel-Twab et al, 2009). Relative to toxicity, there are marked species differences, with the pig being most sensitive to deoxynivalenol, followed by rodent > dog > cat > poultry > ruminants (Rotter et al, 1996).…”