Comparison of the paracrine activity of mesenchymal stem cells derived from human umbilical cord, amniotic membrane and adipose tissue

Dąbrowski, Filip; Burdzińska, Anna; Kulesza, Agnieszka; Śladowska, Anna; Zołocińska, Aleksandra; Gala, Kamila; Pączek, Leszek; Wielgoś, Mirosław

doi:10.1111/jog.13432

Cited by 90 publications

(61 citation statements)

References 43 publications

(97 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, it remains elusive whether the increased levels of MMPs are based on active SC secretion or generated by host cells stimulated by applied SCs. The former mechanism is supported by in vitro data, showing that SCs from different sources possess the capacity to release MMPs at high levels [374][375][376]. However, Mias et al indicated that MSCs modulate the phenotype of cardiac fibroblasts in vitro and promoted the secretion of MMPs [364].…”

Section: Cardiac Remodelingsupporting

confidence: 52%

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Müller

Lemcke

Dávid

2018

Cell Physiol Biochem

174

140

View full text Add to dashboard Cite

A large number of clinical trials have shown stem cell therapy to be a promising therapeutic approach for the treatment of cardiovascular diseases. Since the first transplantation into human patients, several stem cell types have been applied in this field, including bone marrow derived stem cells, cardiac progenitors as well as embryonic stem cells and their derivatives. However, results obtained from clinical studies are inconsistent and stem cell-based improvement of heart performance and cardiac remodeling was found to be quite limited. In order to optimize stem cell efficiency, it is crucial to elucidate the underlying mechanisms mediating the beneficial effects of stem cell transplantation. Based on these mechanisms, researchers have developed different improvement strategies to boost the potency of stem cell repair and to generate the “next generation” of stem cell therapeutics. Moreover, since cardiovascular diseases are complex disorders including several disease patterns and pathologic mechanisms it may be difficult to provide a uniform therapeutic intervention for all subgroups of patients. Therefore, future strategies should aim at more personalized SC therapies in which individual disease parameters influence the selection of optimal cell type, dosage and delivery approach.

show abstract

Section: Cardiac Remodelingsupporting

confidence: 52%

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Müller

Lemcke

Dávid

2018

Cell Physiol Biochem

174

140

View full text Add to dashboard Cite

show abstract

“…An unbiased stable isotope labeling by amino acid-(SILAC-) based quantitative proteomic approach in cell culture coupled to LC-MS and validated by functional assays revealed that the secretome of MSCs isolated from fetal skin is superior to that of adult skin [109]. When considered across different sources, different patterns emerge: the secretion of VEGF and TGF-β1 is higher and comparable in AM-MSCs and AT-MSCs, while CB-MSCs show lower production, with an associated opposite trend for HGF (higher secretion in CB-MSCs vs. AM-MSCs) [110,111]. On the other hand, Kim et al showed increased secretion of 5 Stem Cells International VEGF-A, HGF, bFGF, and ANG-1 from amniotic-derived MSCs when compared to AT-MSCs [112].…”

Section: Role Of Soluble Proteinsmentioning

confidence: 99%

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Maacha

Sidahmed²,

Jacob

et al. 2020

Stem Cells International

182

131

View full text Add to dashboard Cite

The role of the mesenchymal stromal cell- (MSC-) derived secretome is becoming increasingly intriguing from a clinical perspective due to its ability to stimulate endogenous tissue repair processes as well as its effective regulation of the immune system, mimicking the therapeutic effects produced by the MSCs. The secretome is a composite product secreted by MSC in vitro (in conditioned medium) and in vivo (in the extracellular milieu), consisting of a protein soluble fraction (mostly growth factors and cytokines) and a vesicular component, extracellular vesicles (EVs), which transfer proteins, lipids, and genetic material. MSC-derived secretome differs based on the tissue from which the MSCs are isolated and under specific conditions (e.g., preconditioning or priming) suggesting that clinical applications should be tailored by choosing the tissue of origin and a priming regimen to specifically correct a given pathology. MSC-derived secretome mediates beneficial angiogenic effects in a variety of tissue injury-related diseases. This supports the current effort to develop cell-free therapeutic products that bring both clinical benefits (reduced immunogenicity, persistence in vivo, and no genotoxicity associated with long-term cell cultures) and manufacturing advantages (reduced costs, availability of large quantities of off-the-shelf products, and lower regulatory burden). In the present review, we aim to give a comprehensive picture of the numerous components of the secretome produced by MSCs derived from the most common tissue sources for clinical use (e.g., AT, BM, and CB). We focus on the factors involved in the complex regulation of angiogenic processes.

show abstract

“…Adding to that, ADSC might be auto-induced by Tumor Growth Factor-β (TGF-β), TLR2, TNF-αresulting in different inflammatory factors secretion panel as already reported [16,[47][48][49]. In serum-free conditions, ADSC also secreted IL-6, IL-10, and TGF-β [44]. Distinct inflammatory signaling pathways were thus activated impacting the immunosuppressive activity of ADSC.…”

Section: Resultsmentioning

confidence: 70%

“…IL-6 cytokine effects on innate and adaptive immunity have been investigated within their both pro-inflammatory and anti-inflammatory contexts, but mostly presented as a pro-inflammatory cytokine having the ability to dually enforce or suppress immune responses depending on conditions. This cytokine is largely secreted by fresh ADSC than IL-10 and TNF-α [15][16][17]44], however, these concentration levels were reported for fresh expanded ADSC and remained relatively lowers when compared to those of cryopreserved ADSC. Indeed, cryopreservation increased IL-6 and TLR2 at both mRNA and protein levels, especially after P2 expansion.…”

Section: Resultsmentioning

confidence: 83%

Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells

Othmani¹,

Rouam²,

Abbad³

et al. 2019

J Stem Cell Res Ther

View full text Add to dashboard Cite

Comparison of the paracrine activity of mesenchymal stem cells derived from human umbilical cord, amniotic membrane and adipose tissue

Abstract: AM-MSCs show similar IL production as AD-MSCs, while UC-MSCs have a significantly different profile, which suggests diverse biological potential of both cell types for immunomodulative and regenerative therapy.

Cited by 90 publications

References 43 publications

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Stem Cell Therapy in Heart Diseases – Cell Types, Mechanisms and Improvement Strategies

Paracrine Mechanisms of Mesenchymal Stromal Cells in Angiogenesis

Cryopreservation Impacts Cell Functionality of Long Term Expanded Adipose-Derived Stem Cells

Contact Info

Product

Resources

About