This research paper discusses the application of several image-based techniques for measuring optical turbulence. University of Central Florida researchers have previously prototyped and fielded a differential disturbance tracker at the TISTEF 1 kilometer range. This effort has evolved into the development of a software suite that implements image processing techniques such as blob detection, centroid tracking, and optical flow for estimating the refractive index structure parameter. To validate each method, imagery was collected over the 1 kilometer path. The processed results were compared against measurements from an MZA DELTA system.