Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

Okuda, Yo; Ono, Masaaki; Shibata, Isao; Sato, Shizuo; Akashi, Hiroomi

doi:10.1177/104063870601800204

Cited by 42 publications

(50 citation statements)

References 19 publications

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“…Meanwhile, the genome size of our Saudi isolate of FAdV-4 is about 3 kb shorter than that of the North American isolate. Furthermore, the isolate of this study seems to be very close to the newly reported FAdV-4 from China (22,24). The phylogram clearly shows that the reported isolates from eastern Saudi Arabia were clustered together with other Asian strains.…”

Section: Discussionmentioning

confidence: 59%

“…This variation among the FAdV-4 strains may contribute to the viral virulence. The Asian strains seem to be more virulent than the other strains of FAdV-4 reported from North America (3,17,22). Furthermore, the sequencing analysis of the partial hexon gene revealed the high similarity of the FAdV-4 strain isolated in this study to those from Kuwait, India, Pakistan, and China (data not shown).…”

Section: Discussionmentioning

confidence: 79%

“…Restriction endonuclease digestion of fowl adenoviruses is a new tool for the classification of different FAdVs. Furthermore, the combination of PCR and the RFLP of the obtained PCR products is another new tool for FAdV classification (11,22). The RFLP pattern of the reported strain suggests that the circulating strain belongs to the FAdV-4 genotypes (data not shown).…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Hemida¹,

Al-Hammadi²

2017

Turk J Vet Anim Sci

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IntroductionFowl adenoviruses are a diverse group of viruses affecting chickens. They belong to the genus Aviadenovirus in the family Adenoviridae (1). Currently, there are 12 known serotypes of fowl adenoviruses (2). Fowl adenovirus serotype 4 (FAdV-4) belongs to group I and is classified as a member of group C fowl adenoviruses according to restriction fragment length polymorphism (RFLP) (3). FAdV-4 is one of the newly emergent serotypes in many regions worldwide. It was reported in Angora Goth close to Karachi in Pakistan for the first time (4). Clinically, FAdV-4 causes several syndromes in the affected chicken populations such as inclusion body hepatitis and hepatitis-hydropericardium syndrome (5,6). The affected chickens show depression, ruffled feathers, and resting on the ground with their chest close to death (5). Necropsy examination reveals several changes in the liver, such as mottling and being large and pale. Sometimes ecchymotic hemorrhages and multifocal necrotic areas in the parenchyma of the liver are found (7). The clinical picture of FAdV-4-induced hydropericardium syndrome is characterized by the accumulation of jelly-like amber viscous fluid in the pericardial cavities of the affected birds. The infected hearts appear large, flappy, and rounded (7). It is not an easy task to diagnose FAdVs due to the existence of a large number of serotypes. This is in addition to the presence of a heterogeneous population of antibodies against different adenoviruses in the sera of apparently healthy birds. However, the combination of polymerase chain reaction (PCR) and RFLP patterns is one of the most recent trends for the identification of FAdV-4 (8). According to the International Committee of Taxonomy and Nomenclature of Viruses (ICTV), FAdVs were classified into 12 serotypes. Further classification based on RFLP digestion patterns classified them into five groups (A-E) (8,9). Several outbreaks were reported in many Asian countries such as Iraq, India, Japan, and many other countries (10-12). Despite the detection of FAdV-4 in Kuwait (10), nothing is known about its prevalence in the other Gulf countries, especially Saudi Arabia.Several breeder farms reported high morbidity and mortality rates in chickens around 15 weeks old. Birds in these farms showed typical hydropericardium syndrome. Necropsy examination showed that the affected hearts were Abstract: Fowl adenovirus serotype 4 (FAdV-4) is a new emerging viral disease of chickens worldwide. It causes inclusion body hepatitis and hepatitis-hydropericardium syndrome. Little is known about its prevalence in the Middle East. Here we report the prevalence of FAdV-4 in five chicken farms in eastern Saudi Arabia. High mortality rates were reported from birds from those five farms at 15 weeks of age. Gross examination revealed typical hydropericardium syndrome and accumulation of jelly-like materials in the pericardial cavities. We isolated FAdV-4 by using embryonated chicken egg inoculation. The inoculated embryos showed dwarfing, deformities, hemorrhage, an...

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Hemida¹,

Al-Hammadi²

2017

Turk J Vet Anim Sci

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“…Adenovirus infections may cause the clinical form of diseases such as inclusion body hepatitis (IBH) (Singh et al 2006), hydropericardium syndrome (HP) (Geanesh et al 2002), haemorrhagic enteritis virus (HE) (Hess et al 1999, Mazur-Lech et al 2009), Egg drop syndrome (EDS) (Dhinakar et al 2003) or gizzard erosion and ulceration (GEU) (Okuda et al 2006). Such infections play an important role in polietiological infections of the respiratory and digestive tract (Hess 2000, McConnell andFitzgerald 2008) or may proceed without any clinical signs of infection.…”

Section: Introductionmentioning

confidence: 99%

Interactions between Marek’s disease virus Rispens/CVI988 vaccine strain and adenovirus field strain in chicken embryo fibroblast (CEF) cultures

Niczyporuk¹,

Woźniakowski²,

Samorek-Salamonowicz³

et al. 2014

Polish Journal of Veterinary Sciences

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The aim of the study was to determine the influence of adenovirus infection on the replication of Marek's disease virus vaccine strain Rispens/CVI988 during in vitro co-infection studies. Adenovirus field strain JN-5/10j was isolated from sick chickens. The study was conducted in chicken embryo fibroblast cultures (CEF). Monolayers of CEFs were infected with Rispens strain and field adenovirus strain JN-5/10j with different doses (10 1.0 -10 3.0 TCID 50 ) in the following manner: a) simultaneously, b) first, infection with Rispens strain and after 24 h infection with adenovirus strain JN-5/10j and c) infection with adenovirus strain JN-5/10j 24 h before infection with Rispens strain. After 18, 24, 48, 72, and 96 h of incubation, the copy number of the pp38 gene of Rispens strain was determined using Real-time PCR. The results indicated that the Adenovirus infection before the infection with Rispens strain reduced the replication of the pp38 gene after 48 h by 2 log 10 .

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“…In one case they can cause asymptomatic infections, in another they are an aetiological agent of diseases such as inclusion body hepatitis -IBH (6,19) or hydropericardium hepatitis syndrome -HHS (14). The viruses have been also isolated from birds with respiratory tract diseases (22), gizzard erosion and ulceration (GEU) (5,21,23), and pancreatitis (30). Adenoviruses can be the reason of immunodeficiency in young chicks, that can further lead to suppression of the immunity after the vaccination against Marek's disease.…”

mentioning

confidence: 99%

Occurrence of Adenovirus Field Strains in Birds Infected with Marek’S Disease Virus

Niczyporuk

Samorek-Salamonowicz

Czekaj

2012

Bulletin of the Veterinary Institute in Pulawy

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The strains of adenoviruses were isolated from 356 birds with clinical form of Marek's disease and coinfection with adenoviruses. A hexon gene fragment coding loop L1 of adenovirus strains was sequenced and obtained data were analysed with BLAST, Geneious 5.3, and MEGA5 software by comparison with nucleotide sequences of reference strains of fowl adenoviruses (FAdV-1 -FadV-12), two turkey adenoviruses, and two goose adenovirus strains. On this basis, serotypes of adenovirus strains were determined. Sequences of all adenovirus strains isolated from birds infected with Marek's disease virus were classified into six serotypes representing four species. Mostly FAdV-7, FAdV2/11, and FAdV-8a serotypes were found.

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Comparison of the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Pattern of the Fiber Gene and Pathogenicity of Serotype-1 Fowl Adenovirus Isolates from Gizzard Erosions and from Feces of Clinically Healthy Chickens in Japan

Cited by 42 publications

References 19 publications

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Prevalence and molecular characteristics of fowladenovirus serotype 4 in eastern Saudi Arabia

Interactions between Marek’s disease virus Rispens/CVI988 vaccine strain and adenovirus field strain in chicken embryo fibroblast (CEF) cultures

Occurrence of Adenovirus Field Strains in Birds Infected with Marek’S Disease Virus

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