Comparison of the PRAS II, AN-Ident, and RapID-ANA systems for identification of anaerobic bacteria

Abstract

Two rapid systems for the identification of anaerobes were compared to a conventional growth system aided by a computer. The rapid systems (AN-Ident and RapID-ANA) are non-growth-dependent micronmethods that identify anaerobes in 4 h by the action of various constitutive enzymes on chromogenic substrates. The organisms tested were 98 anaerobes, most of which were clinical isolates. The AN!Ident system identified 76 of these to species level and 86 to genus level; the RapID-ANA system correctly identified 74 of the organisms to species level and identified 93 to genus level. The PRAS Il system correctly identified 77 to species level and 96 to genus level. In most instances, adequate identification could be obtained with either of the two rapid systems, but the conventional PRAS II system remains the most accurate.

Evaluation of the An-Ident system and an indole spot test for the rapid differentiation of porcine treponemes

“…Many of these tests detect preformed enzymes and thus yield results rapidly. The miniaturized An-Ident system (Analytab Products, Inc., Saint-Laurent, Quebec, Canada) has been developed and used for the identification of anaerobes of human origin (2,8,17,22,25). In this study, we investigated the possibility of * Corresponding author.…”

Evaluation of API An-IDENT and RapID ANA II systems for identification of Actinomyces species from clinical specimens

“…Initial studies performed on the RapID ANA panel and the An-IDENT reported widely varying percentages of accuracy (2,4,7,8). The wide variations in performance of these systems were related to the numbers, types, and sources (e.g., stock strains, human clinical isolates, and veterinary strains) of organisms tested, whether the additional tests suggested by the system's computer-generated codes were included as a part of the ''rapid'' identification procedure, and whether genus level identifications were considered correct.…”

“…Common characteristics of the two rapid methods used in this study were simplicity of inoculation and the provision of a simple way of screening bacteria for enzyme activities. Results of such tests are rapid, reproducible, and cost-effective (1,2,4,8).…”

Identification ofClostridium botulinumwith API 20 A, Rapid ID 32 A and RapID ANA II

“…Rapid test kits based on phenotypic characteristics of anaerobic bacteria have been developed. Although a number of reports on the suitability of these test systems for identification of different anaerobes have been published [18–23], reports concerning C. botulinum are scarce. Rapid identification tests with high sensitivity and specificity are urgently needed to hasten investigations of botulism outbreaks and to facilitate discrimination between toxigenic and non‐toxigenic strains.…”