Comparison of the Proteomes of Porcine Macrophages and a Stable Porcine Cell Line after Infection with African Swine Fever Virus

Abstract

African swine fever virus (ASFV), causing an OIE-notifiable viral disease of swine, is spreading over the Eurasian continent and threatening the global pig industry. Here, we conducted the first proteome analysis of ASFV-infected primary porcine monocyte-derived macrophages (moMΦ). In parallel to moMΦ isolated from different pigs, the stable porcine cell line WSL-R was infected with a recombinant of ASFV genotype IX strain “Kenya1033”. The outcome of the infections was compared via quantitative mass spectrometry (MS)-based proteome analysis. Major differences with respect to the expression of viral proteins or the host cell response were not observed. However, cell-specific expression of some individual viral proteins did occur. The observed modulations of the host proteome were mainly related to cell characteristics and function. Overall, we conclude that both infection models are suitable for use in the study of ASFV infection in vitro.

Proteome Analysis of Swine Macrophages after Infection with Two Genotype II African Swine Fever Isolates of Different Pathogenicity

“…RIG-I receptor signaling was enhanced in ASFV-Georgia-infected animals and identified as impaired during ASFV infection based on metabolite analysis in vitro [ 15 , 100 ]. Other in vitro analyses described the downregulation of CD14 and CXCR2, an IL8-receptor mediating its signal via G-proteins [ 12 , 37 , 101 , 102 ]. In addition to CD14, in the present study, the downregulation of TLR2 was observed.…”

“…Monocyte-derived macrophages (moMΦ) were isolated, selected, differentiated, and infected with ASFV, as described previously [ 37 ].…”

“…Herein, we analysed the proteomes of primary monocyte-derived macrophages (moMΦ) after infection with two closely related ASFV-genotype II isolates, the highly pathogenic Armenia 2008 [ 36 ] and the moderately pathogenic Estonia 2014 [ 21 ], using reversed-phase high-performance liquid chromatography (HPLC) coupled to a high-resolution trapped ion mobility spectrometry time-of-flight (timsTOF) mass spectrometer. Macrophages were prepared and infected according to a recently established protocol [ 37 ]. One main goal of this study was the characterisation of the expression patterns of viral proteins in the natural target cell and their dependence on the virulence of the respective ASFV strain.…”

Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update

“…The expression profile of viral proteins was very similar in both cell lines, although cell-specific expression of some individual viral proteins did occur [ 120 ]. The authors concluded that both infection models were suitable for investigating ASFV infection biology.…”

“…Nevertheless, a larger number of genes were downregulated in moMΦ compared to WSL cells, suggesting that ASFV induces a host shut-off in moMΦ but no or only a weaker shut-off in WSL cells. This aspect should be taken into account in host interaction studies involving WSL [ 120 ].…”