Comparison of the proteomes of mouse Skin Derived Precursors (SKPs) and SKP‐derived fibroblasts (SFBs) by iTRAQ

Abstract

Skin-derived precursors (SKPs) can differentiate into fibroblasts. However, little is known about the molecular mechanism of the differentiation process. Our previous study has compared the transcriptomes of mouse SKPs and SKP-derived fibroblasts (SFBs) by RNA-Seq analysis and found some genes and signal pathways that might play important roles in the transition from SKPs to SFBs. Here, to further investigate the process by which SKPs differentiate into fibroblasts, we compared the proteomes between SKPs and SFBs using iTRAQ. Our results showed that 243 differentially expressed proteins (DEPs) were identified. Among the 28 DEPs which were related to the functional group of cell differentiation, Down-regulated DEPs collagen XVIII, keratin 10, keratin 5, keratin 14, and TIFIbeta, up-regulated DEPs Thy-1, ANXA1, alpha-catenin, and calreticulin might play important roles in the transition of SKPs to fibroblasts. Further study is needed to clarify the roles of these proteins in the differentiation process of SKPs into fibroblasts, which could facilitate investigations of the detailed molecular mechanisms in the process and make it possible to take advantage of the potential therapeutic applications of SKPs in skin regeneration in the future. K E Y W O R D Sfibroblasts, iTRAQ, proteomes, skin derived precursors 1 | INTRODUCTION Skin-derived precursors (SKPs) from dermis possess the capacities of self-renewal and multipotency. 1,2 Fibroblasts are predominant cells in dermis, which play a pivotal role in maintaining the normal form and function of skin. It has been reported that SKPs can differentiate into fibroblasts. Our previous study 3 showed that SKPs developed into fibroblastlike morphology and expressed fibroblast markers fibronectin, vimentin, and collagen 7 days after being cultured in high glucose DMEM with 10% fetal bovine serum. Biernaskie et al 4,5 reported that SKPs became morphological similar to the endogenous fibroblasts and expressed fibroblast markers after transplanted into dermis. Both in vitro and in vivo experiments proved that SKPs can be induced into fibroblasts, which indicate that SKPs can be used for skin regeneration.It is important to understand the molecular process of SKPs differentiate into fibroblast for the success of cell based therapies. We previously compared the transcriptomes of SKPs and SKP-derived fibroblasts (SFBs) using RNA-Seq. 3 The results showed that nearly 2000 differentially expressed genes (DEGs) were identified. The different expression levels of proteins play an important role in cell growth, environment stimulation reaction, and stem cell differentiation as well.

CD49fhigh Defines a Distinct Skin Mesenchymal Stem Cell Population Capable of Hair Follicle Epithelial Cell Maintenance

“…Out of the 147 homologous genes present in NCSC, 34 were enriched in CD49f high cells (a similarity that is significantly nonstochastic, P < 0.001), such as Ednra, Ngfr, Sox10, and Pax3. Out of 3,368 enriched SKP genes (Mao et al, 2015), a total of 592 genes including those well-validated in previous studies, such as Sox2, Npy, Col18a1, were also present in CD49f high cells (Li et al, 2018;Mao et al, 2018;Zhou et al, 2019).…”

Proteomics Analysis of Testis of Rats Fed a High-Fat Diet

“…We used iTRAQ to investigate the differentially expressed proteins in the testis. Unlike previous studies on single proteins and genes, proteomics studies the disease mechanism from a holistic, multi-faceted, dynamic perspective [7, 8]. We have not only tested the protein expression in the testis tissue, but also conducted gene ontology (GO) analysis and gene and genomic (KEGG) biological pathway analysis to predict the differential expression of long non-coding RNAs (lncRNAs).…”