Comparison of the Q-fever complement fixation test and two commercial enzyme-linked immunosorbent assays for the detection of serum antibodies against<i>Coxiella burnetii</i>(Q-fever) in ruminants: Recommendations for use of serological tests on imported animals in New Zealand

Kittelberger, Reinhold; Mars, Jet; Wibberley, G.; Sting, Reinhard; Henning, Klaus; Horner, G.W.; Garnett, K.M.; Hannah, Michaela J.; Jenner, Judy; Pigott, Clive J.; O’Keefe, John

doi:10.1080/00480169.2009.58619

Cited by 43 publications

(48 citation statements)

References 20 publications

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“…Newly infected young animals could elicit a primary humoral IgM response, not detectable with commercial ELISA kits, with a subsequent delayed IgG humoral response. 22 Indeed, PhI IgM antibody detection was observed in animal F5, and it was not correlated to subsequent IgG seroconversion. Commercial ELISAs capable of detecting anti-PhI and anti-PhII IgM antibodies could improve the detection of serum antibodies in early stages of infection.…”

Section: Discussionmentioning

confidence: 99%

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Coxiella burnetii total immunoglobulin G, phase I and phase II immunoglobulin G antibodies, and bacterial shedding in young dams in persistently infected dairy herds

et al. 2015

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Abstract. The current study examines Coxiella burnetii infection patterns in young dairy dams around the calving period in persistently infected high-producing dairy herds. Infection patterns were determined in terms of total immunoglobulin G (IgG) and phase-specific IgG antibodies by enzyme-linked immunosorbent assay and bacterial shedding by real-time polymerase chain reaction (qPCR). On days 171-177 of gestation, at parturition, and on days 15-21 and 91-97 postpartum, 7 firstparity cows and 7 second-parity cows were sampled for serology and qPCR. Total phase-specific I (PhI) and II (PhII) IgG antibodies were detected in 2 animals at days 171-177 of gestation. Four additional animals underwent seroconversion on days 91-97 postpartum. Three of 6 seropositive dams according to total IgG, showed a PhI+/PhII+ profile, whereas dams that seroconverted exhibited a PhI-/PhII+ (2/6) or PhI+/PhII-(1/6) profile. An indirect fluorescent antibody test for PhI and PhII immunoglobulin M (IgM) was performed on plasma samples from the shedding dams, confirming seropositivity in a first-parity dam that seroconverted, and detecting a sudden spike of PhI-IgM antibodies in 1 further dam. No relationship was detected in young C. burnetii-infected animals between total IgG, PhI and/or PhII antibodies, and bacterial shedding throughout the study period. The highest bacterial load measured by qPCR was recorded in a second-parity dam. This animal presented abnormal peripheral blood counts, which would be an indication of severe peripheral blood alterations in some infected cattle. This study suggests that young shedder cows are mostly seronegative in early stages of infection.

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Section: Discussionmentioning

confidence: 99%

“…21,22,26 However, pathogen detection in cattle is usually challenging because bacterial shedding occurs intermittently and through multiple routes such as birth prod-571993V DIXXX10.1177/1040638715571993Coxiella burnetii infection patterns in young damsSerrano-Pérez et al…”

Section: Introductionmentioning

confidence: 99%

Coxiella burnetii total immunoglobulin G, phase I and phase II immunoglobulin G antibodies, and bacterial shedding in young dams in persistently infected dairy herds

et al. 2015

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“…In this study, we used four commercial ELISAs and one ''in house'' test for the qualitative assessment of seropositivity, as the assays were: (1) reportedly demonstrated to have high levels of accuracy for sero-surveillence compared to gold standard or reference assays; (2) simple and amenable to the testing of large numbers of samples; and (3) affordable and applicable to a non-reference laboratory environment. The IDEXX Q Fever ELISA had a reported sensitivity of 95% and specificity of 100% compared to the reference complement fixation test (Kittelberger 2009). The bovine tuberculosis ELISA used recombinant antigens, and the manufacturer reports sensitivity and specificity of 90.0% and 98.4% (isolation confirmed), and 86.9% and 99.0% (intradermal skin test confirmed), respectively, with antibodies detected 4-8.5 weeks post-infection (BioNote 2011a).…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Major Bovine-Associated Zoonotic Infectious Diseases in the Lao People's Democratic Republic

Vongxay

Conlan

Khounsy

et al. 2012

Vector-Borne and Zoonotic Diseases

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Bovine-associated zoonotic infectious diseases pose a significant threat to human health in the Lao People's Democratic Republic (Lao PDR). In all, 905 cattle and buffalo serum samples collected in northern Lao PDR in 2006 were used to determine seroprevalence of five major bovine zoonotic infectious diseases that included Taenia saginata cysticercosis, bovine tuberculosis, Q-fever, bovine brucellosis, and bovine leptospirosis. Five enzyme-linked immunosorbent assays (ELISAs) were used to test for the presence of antibodies to the diseases, except Taenia saginata, for which we tested for the presence of Taenia metacestode circulating antigens. The overall highest prevalence was for T. saginata (46.4%), with lower prevalence for Q-fever (4%), leptospirosis (3%), tuberculosis (1%), and brucellosis (0.2%). Although there were no significant differences in the proportion of seroprevalence between sex and age of the animals sampled, there were significant differences between the provincial distributions. Further studies are required to determine the seroprevalence of these infections in other locations in Lao PDR, as well as other animal species including humans, in order to develop effective prevention and control strategies. This is the first study to investigate the prevalence of bovine zoonotic infectious agents in the Lao PDR. Positivity was demonstrated for all diseases investigated, with the highest prevalence for T. saginata antigen and Coxiella burnetti antibodies. For T. saginata, there were significant differences in the provincial distribution. Approximately 16% seroprevalence of Coxiella burnetti was noted in Xayabuly Province; however, there are no clear reasons why this was the case, and further studies are required to determine risk factors associated with this observation.

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“…The former method has 92-95% sensitivity and 100% specificity relative to indirect IFA test [17][18][19][20]. It is also economically feasible and easy to perform; therefore, it offers a convenient method for large-scale screening of Q fever antibodies in animals [21]. Ready-to-use ELISA kits are available for determining the prevalence of Q fever in animals.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Q fever in farm animals in Saudi Arabia

Jarelnabi¹,

Alshaikh²,

Bakhiet³

et al. 2018

biomedicalresearch

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This study was undertaken to determine the seroprevalence of Q fever in domestic livestock in Saudi Arabia. Serum samples from 489 camels, 428 cattle, 630 sheep and 423 goats, of either sex, from different localities were tested for antibodies against C. burnetii using a Q fever indirect enzyme-linked immunosorbent assay (ELISA). A subsample of 307 animals of different species was simultaneously tested for C. burnetii antibodies by ELISA and indirect immunofluorescence (IFA). The overall seroprevalence was 30.71%. Prevalence by species was 51.53%, 30.67%, 34.04% and 12.38% in camels, cattle, goats and sheep, respectively. Significant differences in seroprevalence were recorded between species and locations. The prevalence was overtly higher in adult as compared to young animals. No significant difference was recorded between male and female animals. There was a close agreement between ELISA and IFA results in cattle and camels while the results of the two tests were at variance in sheep and goats. The results indicate that the domestic livestock and the camel are the source of Q fever endemicity in Saudi Arabia.

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Comparison of the Q-fever complement fixation test and two commercial enzyme-linked immunosorbent assays for the detection of serum antibodies againstCoxiella burnetii(Q-fever) in ruminants: Recommendations for use of serological tests on imported animals in New Zealand

Cited by 43 publications

References 20 publications

Coxiella burnetii total immunoglobulin G, phase I and phase II immunoglobulin G antibodies, and bacterial shedding in young dams in persistently infected dairy herds

Coxiella burnetii total immunoglobulin G, phase I and phase II immunoglobulin G antibodies, and bacterial shedding in young dams in persistently infected dairy herds

Seroprevalence of Major Bovine-Associated Zoonotic Infectious Diseases in the Lao People's Democratic Republic

Seroprevalence of Q fever in farm animals in Saudi Arabia

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