Comparison of the relative sensitivity of human lymphocytes and mouse splenocytes to two spindle poisons

Steiblen, Guy; Orsière, Thierry; Pallen, Catherine; Botta, A.; Marzin, Daniel

doi:10.1016/j.mrgentox.2005.10.004

Cited by 13 publications

(4 citation statements)

References 19 publications

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“…The results were comparable with those reported by Elhajouji et al (13), who found that under the same experimental conditions, the NOEL of nocodazole was 33 nM in human lymphocytes. For paclitaxel, the NOEL was 0.5 nM in mouse splenocytes, while it was 2.5 nM in human lymphocytes, which indicates a higher degree of sensitivity of the mouse splenocyte compared with the human lymphocyte in this case (16).…”

Section: Aneugensmentioning

confidence: 77%

Potential thresholds for genotoxic effects by micronucleus scoring

Elhajouji¹,

Lukamowicz²,

Cammerer

et al. 2010

Mutagenesis

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The concept of thresholds in genotoxicity has been open for debate in the last decades. The micronucleus (MN) test contributed to a large extent in understanding the dose-response relationship for aneugens and clastogens. The threshold concept for aneuploidy is well accepted by the scientific community based on the data and for mechanistic reasons. The concept of threshold for clastogens is still challenging. Acceptance is based on a case-by-case basis together with thorough mechanistic understanding of the different steps from the mutagen-target interactions to MN formation for this class of genotoxicants. This review summarises the significant achievements in the assessment of threshold for genotoxins using the MN test and concludes with an overview of knowledge gaps and recommendations.

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Section: Aneugensmentioning

confidence: 77%

Potential thresholds for genotoxic effects by micronucleus scoring

Elhajouji¹,

Lukamowicz²,

Cammerer

et al. 2010

Mutagenesis

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“…The study was performed according to ISO standards 14 on human lymphocytes because of its sensitivity. 15 Fresh whole blood collected by venipuncture (0.7 mL) was added to vials containing 9.3 mL of culture medium (X-VIVO 10), supplemented with 1% phytohemagglutinin (Life Technologies, Paisley, UK), which is a specific mitogen to induce T-lymphocyte proliferation and 1% heparin (Life Technologies) to prevent blood coagulation. These vials were incubated at 37°C in a humidified atmosphere containing 5% CO 2 .…”

Section: Genotoxicity Testmentioning

confidence: 99%

Biological properties of a neutralized 2.5% sodium hypochlorite solution

Aubut

Pommel²,

Verhille³

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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“…Micronuclei formation was also observed in cultured mouse splenocytes and human lymphocytes treated with nocodazole (Steiblen et al, 2005). In vivo micronucleus induction in mouse bone marrow by nocodazole was also reported (Tinwell and Ashby, 1991).…”

Section: Introductionmentioning

confidence: 82%