Comparison of the response of bovine and human neutrophils to various stimuli

Tulathromycin Exerts Proresolving Effects in Bovine Neutrophils by Inhibiting Phospholipases and Altering Leukotriene B4, Prostaglandin E2, and Lipoxin A4Production

“…In contrast with its effects on human neutrophils, PMA stimulation did not significantly increase PLD activity in bovine neutrophils. This is consistent with the previously observed species-dependent effects of PMA (57,58). Having reported that tulathromycin activates caspase-3 in bovine neutrophils, future studies should elucidate whether the effect of tulathromycin on PLD activity is caspase dependent or whether it occurs via a separate mechanism.…”

Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

“…In support of this conclusion, PAF stimulation in the absence of cytochalasin B in human neutrophils resulted in the release of very little, if any, azurophil granule enzymes [14,38]. In addition, it has been reported that cytochalasin B itself can stimulate release of azurophil granule enzymes from bovine neutrophils [19]. Thus, we chose not to use cytochalasin B in our experiments so that we would be able to compare the relative mobilization of different granules to comparable concentrations of PAF under similar and physiologically relevant conditions.…”

“…Previously, it has been shown that bovine neutrophil respiratory burst activity can be stimulated by a number of agonists, including protein kinase C activators (e.g., PMA) [19,44,56,66,67], opsonized zymosan [19,46,56,68], heat-aggregated bovine IgG [45,46], and bovine IL-1 [57,69]. In the present studies, we found that PAF was, in contrast, a very weak stimulus for ROS production by isolated bovine neutrophils and could only detect significant levels of ROS production after treatment with PAF concentrations of 10 -6 M or higher.…”

“…In addition, bovine neutrophils contain much lower concentrations of lysozyme as compared to human neutrophils [18]. However, one of the most unusual features of bovine neutrophils is their lack of response to N-formyl chemotactic peptides, reportedly due to the absence of detectable N-formyl peptide receptors [19,20]. Because of these differences, the biological responses of bovine neutrophils to inflammatory agents may not necessarily be identical to those of human cells.…”

Evaluation of the Influence of Potential Toxins on Neutrophil Function

“…The basic assays listed in Table I generally work in the various species in which they have been attempted. However, modifications to the assays are often required for each individual species (3,7,8,10,34). There are many methods for isolating purified neutrophils from peripheral blood, and these methods are often species-specific (7, 10, 11, 14, 17-19, 22, 23).…”