Comparison of the retention of basic compounds in anionic and cationic microemulsion electrokinetic chromatographic systems

Fernández-Pumarega, Alejandro; Olmo, Laura; Amézqueta, Susana; Fuguet, Elisabet; Rosés, Martı́

doi:10.1016/j.microc.2020.105259

Cited by 1 publication

(1 citation statement)

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“…Because MEKC is also often used with organic modifiers to increase the solubility for hydrophobic analytes, it should be considered that the CMC of surfactants (including that of SDS or sodium lauryl ether sulfate) in most BGE will be different (typically lower) than the corresponding value in a pure aqueous solutions [68, 69]. In this regard, the pH of the BGE should also be carefully monitored, because it may not only determine if the migration of the analyte will be affected by electrostatic interactions with the surface of the micelle but may also affect the aggregation of the surfactant monomers [70].…”

Section: Methods Development For Optimal Separationsmentioning

confidence: 99%

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

et al. 2023

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This review is in support of the development of selective, precise, fast, and validated capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H, Degenhardt M, Kunkel A. “Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications,” pointing out which fundamentals are still valid and at the same time showing the enormous achievements in the last 25 years. The structures of both reviews are widely similar, in order to facilitate their simultaneous use. Focusing on pharmaceutical and biological applications, the successful use of CE is now demonstrated by more than 600 carefully selected references. Many of those are recent reviews; therefore, a significant overview about the field is provided. There are extra sections about sample pretreatment related to CE and microchip CE, and a completely revised section about method development for protein analytes and biomolecules in general. The general strategies for method development are summed up with regard to selectivity, efficiency, precision, analysis time, limit of detection, sample pretreatment requirements, and validation.

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Section: Methods Development For Optimal Separationsmentioning

confidence: 99%