2013
DOI: 10.5219/311
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of the sensitivity of determining soyeabean allergens by ELISA method and SYBR green I

Abstract: The aim of this study was to compare the suitability of two methods for detecting defatted soybean powder; SYBR Green I Real-time PCR and enzyme-linked immunosorbent assay (ELISA). Analysis of 20 artificially contaminated samples prepared by simple dilution with wheat flour revealed that both techniques were able to detect defatted soybean powder, although there were significant differences between the two methods. Wheat flour contamination with defatted soybean powder was detected in samples 1-5, (0.012 %; 12… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2014
2014
2014
2014

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 15 publications
0
1
0
Order By: Relevance
“…Real-time PCR is also a useful tool for detection: gene expression [19] identification and quantification of pathogens or allergens, [20][21][22] and authentication of food. [23,24] The aim of the study was to determinate and compare relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in pollen samples from different Ukraine areas by real-time PCR.…”
Section: Introductionmentioning
confidence: 99%
“…Real-time PCR is also a useful tool for detection: gene expression [19] identification and quantification of pathogens or allergens, [20][21][22] and authentication of food. [23,24] The aim of the study was to determinate and compare relative quantity of hazelnut allergens Corylus avellana, L. CorA and Corylus avellana, L. pollen profiling in pollen samples from different Ukraine areas by real-time PCR.…”
Section: Introductionmentioning
confidence: 99%