Zinc (Zn), copper (Cu), and selenium (Se) are well-known as essential trace elements. These elements attract a great interest, not only in the scientific world, but also in the public option. The aim of this study was to evaluate the serum Zn, Cu, and Se levels in healthy volunteers (60 females and 55 males) living in Tehran. The age (6-62 years) and sex of the subjects, as well as drug and tobacco consumption, were registered. The concentrations of these elements were determined by atomic absorption spectrometry. The serum levels of Zn, Cu, and Se in the normal population of Tehran was 0.89 +/- 0.16 mg/L, 0.95 +/- 0.20 mg/L, and 99.10 +/- 21.78 microg/L, respectively. There was no significant gender-related difference in serum Zn concentration (p < 0.15). However, a significant difference (p < 0.05) in both Cu and Se levels was observed between males and females.